(Circular) (Six-base) MAPSORT of: pFN21AE3642.seq Check: 6184 from: 1 to: 5653 pFN21AE3642 5653 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2027 2547 2027 Size: 520 5133 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 2996 1937 Size: 1002 57 4594 Fragments arranged by size: 4594 1002 57 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2343 3716 5100 5368 1632 Size: 711 1373 1384 268 1917 Fragments arranged by size: 1917 1384 1373 711 268 AfeI AGC'GCT Cuts at: 2900 2900 Size: 5653 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2129 1166 Size: 963 4690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2207 2689 1184 Size: 1023 482 4148 Fragments arranged by size: 4148 1023 482 AlwNI CAG_nnn'CTG Cuts at: 2350 3396 4968 2350 Size: 1046 1572 3035 Fragments arranged by size: 3035 1572 1046 ApaI G_GGCC'C Cuts at: 2140 2140 Size: 5653 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2723 2962 3188 3340 5449 1493 Size: 1230 239 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 239 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2978 1870 Size: 73 1035 4545 Fragments arranged by size: 4545 1035 73 BamHI G'GATC_C Cuts at: 2214 2983 3350 2214 Size: 769 367 4517 Fragments arranged by size: 4517 769 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 2974 4429 618 Size: 325 217 627 459 728 1455 1842 Fragments arranged by size: 1842 1455 728 627 459 325 217 BanII G_rGCy'C Cuts at: 1391 2140 2972 1391 Size: 749 832 4072 Fragments arranged by size: 4072 832 749 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 4264 136 Size: 107 122 71 631 605 367 2225 1525 Fragments arranged by size: 2225 1525 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 2625 5648 2625 Size: 3023 2630 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2030 2140 2374 3714 4870 1790 Size: 240 110 234 1340 1156 2573 Fragments arranged by size: 2573 1340 1156 240 234 110 BmgBI CAC'GTC Cuts at: 1318 2893 1318 Size: 1575 4078 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2835 3384 4342 Size: 416 53 83 399 24 1418 549 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 1418 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 1239 Size: 3074 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 3778 4643 4941 2147 Size: 1631 865 298 2859 Fragments arranged by size: 2859 1631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2849 4316 882 Size: 617 1350 1467 2219 Fragments arranged by size: 2219 1467 1350 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 1178 Size: 30 165 30 1152 30 4246 Fragments arranged by size: 4246 1152 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2085 2453 2456 2762 2867 1186 Size: 899 368 3 306 105 3972 Fragments arranged by size: 3972 899 368 306 105 3 BseYI C'CCAG_C Cuts at: 2029 4856 5352 2029 Size: 2827 496 2330 Fragments arranged by size: 2827 2330 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 3103 3864 4892 1540 Size: 275 186 589 513 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 3714 3799 4870 2598 Size: 1116 85 1071 3381 Fragments arranged by size: 3381 1116 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2140 2374 2598 2972 3714 3799 Size: 399 240 110 234 224 374 742 85 Cuts at: 3799 4870 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 240 234 224 110 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2027 2547 3008 844 Size: 1183 520 461 3489 Fragments arranged by size: 3489 1183 520 461 BsrBI CCG'CTC Cuts at: 1394 1823 2446 3540 1394 Size: 429 623 1094 3507 Fragments arranged by size: 3507 1094 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2295 2919 4297 4484 1700 Size: 110 485 624 1378 187 2869 Fragments arranged by size: 2869 1378 624 485 187 110 BsrGI T'GTAC_A Cuts at: 96 2487 96 Size: 2391 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2214 2625 2983 3350 3735 3752 5204 5290 Size: 242 411 358 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 411 385 367 358 346 242 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2035 2035 Size: 5653 BtgI C'CryG_G Cuts at: 513 1065 2472 2697 513 Size: 552 1407 225 3469 Fragments arranged by size: 3469 1407 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2930 487 Size: 683 56 1704 3210 Fragments arranged by size: 3210 1704 683 56 BtsI GCAGTG_nn' Cuts at: 3169 3963 3983 3169 Size: 794 20 4839 Fragments arranged by size: 4839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2038 2073 529 Size: 35 1474 35 4109 Fragments arranged by size: 4109 1474 35 35 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2587 2647 2929 Size: 54 1425 50 275 18 757 60 282 Cuts at: 2929 3100 3989 5618 8 Size: 171 889 1629 43 Fragments arranged by size: 1629 1425 889 757 282 275 171 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2587 3100 1537 Size: 275 775 513 4090 Fragments arranged by size: 4090 775 513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 3583 1353 Size: 1250 980 3423 Fragments arranged by size: 3423 1250 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2870 4240 4614 4760 387 Size: 1064 138 1281 1370 374 146 1280 Fragments arranged by size: 1370 1281 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 3716 4313 5100 5368 1239 Size: 393 711 1373 597 787 268 1524 Fragments arranged by size: 1524 1373 787 711 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2435 2342 Size: 93 5560 EcoO109I rG'GnC_Cy Cuts at: 2137 2137 Size: 5653 EcoRI G'AATT_C Cuts at: 2723 2962 3340 2723 Size: 239 378 5036 Fragments arranged by size: 5036 378 239 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2902 4800 1678 Size: 1224 1898 2531 Fragments arranged by size: 2531 1898 1224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 4294 4326 4368 Size: 32 135 32 983 32 1707 32 42 Cuts at: 4368 4400 1373 Size: 32 2626 Fragments arranged by size: 2626 1707 983 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2784 4767 4951 1305 Size: 1479 1983 184 2007 Fragments arranged by size: 2007 1983 1479 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2649 10 Size: 54 1425 343 817 3014 Fragments arranged by size: 3014 1425 817 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2129 2189 3611 3970 4129 518 Size: 648 963 60 1422 359 159 2042 Fragments arranged by size: 2042 1422 963 648 359 159 60 MspA1I CmG'CkG Cuts at: 1606 3069 3746 4894 5139 1606 Size: 1463 677 1148 245 2120 Fragments arranged by size: 2120 1463 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5101 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NruI TCG'CGA Cuts at: 2949 2949 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 1108 Size: 1242 4411 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2136 2136 Size: 5653 PstI C_TGCA'G Cuts at: 830 2379 3005 830 Size: 1549 626 3478 Fragments arranged by size: 3478 1549 626 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2603 2603 Size: 5653 SbfI CC_TGCA'GG Cuts at: 2379 3005 2379 Size: 626 5027 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2375 2902 3001 4136 4817 5008 826 Size: 219 1330 527 99 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 681 527 219 191 99 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 3757 4658 4920 820 Size: 197 926 219 1595 901 262 1553 Fragments arranged by size: 1595 1553 926 901 262 219 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2715 3092 513 Size: 552 1650 377 3074 Fragments arranged by size: 3074 1650 552 377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2213 3680 3697 3850 4035 4480 325 Size: 1255 633 1467 17 153 185 445 1498 Fragments arranged by size: 1498 1467 1255 633 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2487 3899 Size: 274 80 33 51 1443 141 369 1412 Cuts at: 3899 5611 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1412 369 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 5613 5645 2745 Size: 32 2836 32 2753 Fragments arranged by size: 2836 2753 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2876 1316 Size: 1560 4093 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2470 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI