Restriction map for
pFN21AE3642
(Linear) (Six-base) MAPSORT of: KIEE3642.seq Check: 94 from: 1 to: 948
KIEE3642.orf
With 182 enzymes: SgfI *
December 27, 2011 16:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 22 542 948
Size: 22 520 406
Fragments arranged by size:
520 406 22
AccI GT'mk_AC
Cuts at: 0 934 948
Size: 934 14
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 948
Size: 338 610
AfeI AGC'GCT
Cuts at: 0 895 948
Size: 895 53
AleI CACnn'nnGTG
Cuts at: 0 124 948
Size: 124 824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 684 948
Size: 202 482 264
Fragments arranged by size:
482 264 202
AlwNI CAG_nnn'CTG
Cuts at: 0 345 948
Size: 345 603
ApaI G_GGCC'C
Cuts at: 0 135 948
Size: 135 813
ApoI r'AATT_y
Cuts at: 0 718 948
Size: 718 230
BamHI G'GATC_C
Cuts at: 0 209 948
Size: 209 739
BanI G'GyrC_C
Cuts at: 0 241 948
Size: 241 707
BanII G_rGCy'C
Cuts at: 0 135 948
Size: 135 813
BglI GCCn_nnn'nGGC
Cuts at: 0 34 948
Size: 34 914
BglII A'GATC_T
Cuts at: 0 620 948
Size: 620 328
Bme1580I G_kGCm'C
Cuts at: 0 25 135 369 948
Size: 25 110 234 579
Fragments arranged by size:
579 234 110 25
BmgBI CAC'GTC
Cuts at: 0 888 948
Size: 888 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 830 948
Size: 830 118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 948
Size: 142 806
BsaI GGTCTCn'nnnn_
Cuts at: 0 844 948
Size: 844 104
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 948
Size: 550 30 368
Fragments arranged by size:
550 368 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 80 448 451 757 862 948
Size: 80 368 3 306 105 86
Fragments arranged by size:
368 306 105 86 80 3
BseYI C'CCAG_C
Cuts at: 0 24 948
Size: 24 924
BsiEI CG_ry'CG
Cuts at: 0 585 948
Size: 585 363
BsiHKAI G_wGCw'C
Cuts at: 0 593 948
Size: 593 355
Bsp1286I G_dGCh'C
Cuts at: 0 25 135 369 593 948
Size: 25 110 234 224 355
Fragments arranged by size:
355 234 224 110 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 22 542 948
Size: 22 520 406
Fragments arranged by size:
520 406 22
BsrBI CCG'CTC
Cuts at: 0 441 948
Size: 441 507
BsrFI r'CCGG_y
Cuts at: 0 290 914 948
Size: 290 624 34
Fragments arranged by size:
624 290 34
BsrGI T'GTAC_A
Cuts at: 0 482 948
Size: 482 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 948
Size: 468 480
BstYI r'GATC_y
Cuts at: 0 209 620 948
Size: 209 411 328
Fragments arranged by size:
411 328 209
Bsu36I CC'TnA_GG
Cuts at: 0 30 948
Size: 30 918
BtgI C'CryG_G
Cuts at: 0 467 692 948
Size: 467 225 256
Fragments arranged by size:
467 256 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 925 948
Size: 925 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 33 68 948
Size: 33 35 880
Fragments arranged by size:
880 35 33
EaeI y'GGCC_r
Cuts at: 0 582 642 924 948
Size: 582 60 282 24
Fragments arranged by size:
582 282 60 24
EagI C'GGCC_G
Cuts at: 0 582 948
Size: 582 366
EarI CTCTTCn'nnn_
Cuts at: 0 598 948
Size: 598 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 948
Size: 865 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 948
Size: 338 610
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 430 948
Size: 337 93 518
Fragments arranged by size:
518 337 93
EcoO109I rG'GnC_Cy
Cuts at: 0 132 948
Size: 132 816
EcoRI G'AATT_C
Cuts at: 0 718 948
Size: 718 230
HaeII r_GCGC'y
Cuts at: 0 897 948
Size: 897 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 948
Size: 550 32 366
Fragments arranged by size:
550 366 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 948
Size: 779 169
MscI TGG'CCA
Cuts at: 0 644 948
Size: 644 304
MslI CAynn'nnrTG
Cuts at: 0 124 184 948
Size: 124 60 764
Fragments arranged by size:
764 124 60
NruI TCG'CGA
Cuts at: 0 944 948
Size: 944 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 345 948
Size: 345 603
PspOMI G'GGCC_C
Cuts at: 0 131 948
Size: 131 817
PstI C_TGCA'G
Cuts at: 0 374 948
Size: 374 574
SapI GCTCTTCn'nnn_
Cuts at: 0 598 948
Size: 598 350
SbfI CC_TGCA'GG
Cuts at: 0 374 948
Size: 374 574
SfcI C'TryA_G
Cuts at: 0 370 897 948
Size: 370 527 51
Fragments arranged by size:
527 370 51
SmlI C'TyrA_G
Cuts at: 0 157 948
Size: 157 791
StyI C'CwwG_G
Cuts at: 0 710 948
Size: 710 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 948
Size: 208 740
TatI w'GTAC_w
Cuts at: 0 113 482 948
Size: 113 369 466
Fragments arranged by size:
466 369 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 740 772 948
Size: 740 32 176
Fragments arranged by size:
740 176 32
Tth111I GACn'n_nGTC
Cuts at: 0 871 948
Size: 871 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 948
Size: 465 483
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI AlwNI ApaI
ApoI BamHI BanI BanII BglI BglII Bme1580I BmgBI
BmrI BpuEI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstXI BstYI Bsu36I
BtgI BtgZI CspCI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MscI MslI
NruI PflMI PspOMI PstI SapI SbfI SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI
BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtsI ClaI DraI DraIII
DrdI EcoICRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI XmnI ZraI