Restriction map for
pFN21AE2363
##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflIII AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII BamHI BbvCI BcgI BciVI
BclI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI Ksp632I KspI MfeI
MluI MroI MunI MvnI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 349 GTCTAC GT^[AC][GT]AC
AcyI 278 GGCGCC G[GA]^CG[CT]C
AflII 1495 CTTAAG C^TTAAG
AgeI 781 ACCGGT A^CCGGT
AlwNI 1264 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1078 CCTCAGG CC^T[ACGT]AGG
AvrII 1110 CCTAGG C^CTAGG
BbeI 278 GGCGCC GGCGC^C
BbrPI 199 CACGTG CAC^GTG
BbsI 153 GAAGAC GAAGAC(2/6)
BfrI 1495 CTTAAG C^TTAAG
BglI 32 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1247 AGATCT A^GATCT
BlnI 1110 CCTAGG C^CTAGG
BlpI 27 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 27 GCTTAGC GC^T[ACGT]AGC
BpuAI 153 GAAGAC GAAGAC(2/6)
BsaAI 199 CACGTG [CT]AC^GT[GA]
BsaBI 223 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 278 GGCGCC G[GA]^CG[CT]C
BsaI 367 GAGACC GGTCTC(1/5)
BsaWI 781 ACCGGT [AT]^CCGG[AT]
BsmAI 367 GAGAC GTCTC(1/5)
BsrI 948 CCAGT ACTGG(1/-1)
Bsu36I 1078 CCTCAGG CC^T[ACGT]AGG
CelII 27 GCTTAGC GC^T[ACGT]AGC
Eco81I 1078 CCTCAGG CC^T[ACGT]AGG
EcoNI 669 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 278 GGCGCC [GA]GCGC^[CT]
Hin1I 278 GGCGCC G[GA]^CG[CT]C
KasI 278 GGCGCC G^GCGCC
MaeII 200 ACGT A^CGT
MamI 223 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 305 GCCGGC GCC^GGC
NarI 278 GGCGCC GG^CGCC
NgoMIV 305 GCCGGC G^CCGGC
NspI 903 GCATGC [GA]CATG^[CT]
PflMI 750 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 781 ACCGGT A^CCGGT
PmaCI 199 CACGTG CAC^GTG
PmlI 199 CACGTG CAC^GTG
PpuMI 268 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 1318 ACCAGGT A^CC[AT]GGT
SfoI 278 GGCGCC GGC^GCC
SphI 903 GCATGC GCATG^C
StuI 1499 AGGCCT AGG^CCT
Tsp509I 1233 AATT ^AATT
Van91I 750 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 441 GTAC GT^AC
1371 GTAC
Asp700I 153 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
384 GAAGCCTTTC
BanI 15 GGCACC G^G[CT][GA]CC
278 GGCGCC
BanII 520 GAGCCC G[GA]GC[CT]^C
867 GAGCCC
BfaI 331 CTAG C^TAG
1111 CTAG
BsgI 1216 GTGCAG GTGCAG(16/14)
1261 GTGCAG
BsmFI 1396 GGGAC GGGAC(10/14)
270 GTCCC
BspMI 1429 ACCTGC ACCTGC(4/8)
733 GCAGGT
BsrBI 298 GAGCGG CCGCTC(-3/-3)
337 GAGCGG
BsrFI 305 GCCGGC [GA]^CCGG[CT]
781 ACCGGT
BstEII 211 GGTGACC G^GT[ACGT]ACC
1521 GGTCACC
BstPI 211 GGTGACC G^GT[ACGT]ACC
1521 GGTCACC
BstXI 479 CCATCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
788 CCACCGAGGTGG
BstYI 1247 AGATCT [GA]^GATC[CT]
1353 GGATCT
Cfr10I 305 GCCGGC [GA]^CCGG[CT]
781 ACCGGT
DraII 268 GGGTCCC [GA]G^G[ACGT]CC[CT]
1074 GGGGCCT
EcoO109I 268 GGGTCCC [GA]G^G[ACGT]CC[CT]
1074 GGGGCCT
EcoO65I 211 GGTGACC G^GT[ACGT]ACC
1521 GGTCACC
HincII 169 GTCAAC GT[CT]^[GA]AC
763 GTTGAC
HindII 169 GTCAAC GT[CT]^[GA]AC
763 GTTGAC
MaeI 331 CTAG C^TAG
1111 CTAG
MflI 1247 AGATCT [GA]^GATC[CT]
1353 GGATCT
MseI 1454 TTAA T^TAA
1496 TTAA
MspA1I 112 CAGCTG C[AC]G^C[GT]G
818 CAGCTG
NcoI 274 CCATGG C^CATGG
485 CCATGG
PaeR7I 364 CTCGAG C^TCGAG
1006 CTCGAG
PvuII 112 CAGCTG CAG^CTG
818 CAGCTG
RsaI 441 GTAC GT^AC
1371 GTAC
SmaI 248 CCCGGG CCC^GGG
1349 CCCGGG
Tru9I 1454 TTAA T^TAA
1496 TTAA
XhoI 364 CTCGAG C^TCGAG
1006 CTCGAG
XhoII 1247 AGATCT [GA]^GATC[CT]
1353 GGATCT
XmaCI 248 CCCGGG C^CCGGG
1349 CCCGGG
XmaI 248 CCCGGG C^CCGGG
1349 CCCGGG
XmnI 153 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
384 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BalI BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI