Restriction map for
pFN21AB3692
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# NO CUT ENZYMES ( 199 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DpnII DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MboI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 220 GACGTC GACGT^C
AciI 231 CCGC CCGC(-3/-1)
AcyI 220 GACGTC G[GA]^CG[CT]C
AfaI 255 GTAC GT^AC
AvaII 273 GGACC G^G[AT]CC
BbsI 52 GTCTTC GAAGAC(2/6)
BpmI 369 CTGGAG CTGGAG(16/14)
BpuAI 52 GTCTTC GAAGAC(2/6)
BsaHI 220 GACGTC G[GA]^CG[CT]C
BsaJI 9 CCAAGG C^C[ACGT][ACGT]GG
BsmAI 152 GTCTC GTCTC(1/5)
BsmFI 310 GGGAC GGGAC(10/14)
BsrDI 205 CATTGC GCAATG(2/0)
Cac8I 74 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 273 GGACC G^G[ACGT]CC
DraII 272 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 272 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 9 CCAAGG C^C[AT][AT]GG
FauI 230 CCCGC CCCGC(4/6)
Hin1I 220 GACGTC G[GA]^CG[CT]C
HphI 345 GGTGA GGTGA(8/7)
MfeI 126 CAATTG C^AATTG
MseI 287 TTAA T^TAA
MunI 126 CAATTG C^AATTG
MwoI 74 GCCTGCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 371 GGAGCC GG[ACGT]^[ACGT]CC
PleI 348 GACTC GAGTC(4/5)
PpuMI 272 AGGACCT [GA]G^G[AT]CC[CT]
PstI 76 CTGCAG CTGCA^G
RsaI 255 GTAC GT^AC
Sau96I 273 GGACC G^G[ACGT]CC
SfaNI 145 GATGC GCATC(5/9)
SfcI 76 CTGCAG C^T[GA][CT]AG
SmlI 234 CTTGAG C^T[CT][GA]AG
StyI 9 CCAAGG C^C[AT][AT]GG
TfiI 5 GAATC G^A[AT]TC
Tru9I 287 TTAA T^TAA
TspRI 79 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BssKI 45 CCTGG ^CC[ACGT]GG
368 CCTGG
BstF5I 314 CATCC GGATG(2/0)
379 CATCC
BstNI 45 CCTGG CC^[AT]GG
368 CCTGG
EcoRII 45 CCTGG ^CC[AT]GG
368 CCTGG
FokI 314 CATCC GGATG(9/13)
379 CATCC
HinfI 5 GAATC G^A[ACGT]TC
348 GACTC
MaeII 221 ACGT A^CGT
253 ACGT
MboII 53 TCTTC GAAGA(8/7)
336 TCTTC
MnlI 14 GAGG CCTC(7/6)
237 GAGG
MvaI 45 CCTGG CC^[AT]GG
368 CCTGG
ScrFI 45 CCTGG CC^[ACGT]GG
368 CCTGG
Tsp45I 223 GTCAC ^GT[GC]AC
346 GTGAC
Tsp509I 127 AATT ^AATT
211 AATT
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
AluI BbvI BsiYI BslI Fnu4HI
ItaI MaeIII TseI