################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI BbsI BbvCI BcgI BciVI BclI BfrI BglII BpuAI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MamI MfeI MluI MroI MseI MunI NaeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 832 GTCTAC GT^[AC][GT]AC Aor51HI 896 AGCGCT AGC^GCT AspHI 1211 GTGCTC G[AT]GC[AT]^C AviII 1199 TGCGCA TGC^GCA AvrII 1206 CCTAGG C^CTAGG BamHI 986 GGATCC G^GATCC BbrPI 1287 CACGTG CAC^GTG BlnI 1206 CCTAGG C^CTAGG BsaAI 1287 CACGTG [CT]AC^GT[GA] BsaI 268 GGTCTC GGTCTC(1/5) BseRI 1051 CTCCTC GAGGAG(10/8) BsgI 24 CTGCAC GTGCAG(16/14) BsiEI 159 CGGCCG CG[GA][CT]^CG BsiHKAI 1211 GTGCTC G[AT]GC[AT]^C BsmBI 670 GAGACG CGTCTC(1/5) BsmFI 1065 GGGAC GGGAC(10/14) BsmI 283 GCATTC GAATGC(1/-1) BspMI 641 ACCTGC ACCTGC(4/8) BssSI 773 CTCGTG CACGAG(-5/-1) BstEII 72 GGTCACC G^GT[ACGT]ACC BstPI 72 GGTCACC G^GT[ACGT]ACC BstYI 986 GGATCC [GA]^GATC[CT] DraIII 1284 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 159 CGGCCG C^GGCCG EclXI 159 CGGCCG C^GGCCG Eco47III 896 AGCGCT AGC^GCT Eco52I 159 CGGCCG C^GGCCG EcoNI 915 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 72 GGTCACC G^GT[ACGT]ACC FspI 1199 TGCGCA TGC^GCA KspI 1226 CCGCGG CCGC^GG MflI 986 GGATCC [GA]^GATC[CT] MspA1I 1226 CCGCGG C[AC]G^C[GT]G NcoI 953 CCATGG C^CATGG NdeI 389 CATATG CA^TATG PmaCI 1287 CACGTG CAC^GTG PmlI 1287 CACGTG CAC^GTG PpuMI 782 AGGACCC [GA]G^G[AT]CC[CT] SacII 1226 CCGCGG CCGC^GG SapI 624 GCTCTTC GCTCTTC(1/4) SfiI 423 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 139 CCCGGG CCC^GGG SmlI 1354 CTCAAG C^T[CT][GA]AG StuI 1274 AGGCCT AGG^CCT Tsp45I 73 GTCAC ^GT[GC]AC XhoII 986 GGATCC [GA]^GATC[CT] XmaCI 139 CCCGGG C^CCGGG XmaI 139 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 347 GGCGCC G[GA]^CG[CT]C 693 GGCGCC AlwI 987 GATCC GGATC(4/5) 986 GGATC AlwNI 298 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1327 CAGAAGCTG AvaI 139 CCCGGG C^[CT]CG[GA]G 858 CTCGGG BbeI 347 GGCGCC GGCGC^C 693 GGCGCC BfaI 829 CTAG C^TAG 1207 CTAG BglI 424 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 818 GCCGCCTGGGC BsaHI 347 GGCGCC G[GA]^CG[CT]C 693 GGCGCC BsoBI 139 CCCGGG C^[CT]CG[GA]G 858 CTCGGG DpnII 730 GATC ^GATC 987 GATC EcoT14I 953 CCATGG C^C[AT][AT]GG 1206 CCTAGG HgaI 672 GACGC GACGC(5/10) 1350 GACGC Hin1I 347 GGCGCC G[GA]^CG[CT]C 693 GGCGCC KasI 347 GGCGCC G^GCGCC 693 GGCGCC MaeI 829 CTAG C^TAG 1207 CTAG MaeIII 73 GTCAC ^GT[ACGT]AC 1337 GTTAC MboI 730 GATC ^GATC 987 GATC NarI 347 GGCGCC GG^CGCC 693 GGCGCC NdeII 730 GATC ^GATC 987 GATC PflMI 313 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 787 CCACCCTCTGG PstI 295 CTGCAG CTGCA^G 1324 CTGCAG Sau3AI 730 GATC ^GATC 987 GATC SfoI 347 GGCGCC GGC^GCC 693 GGCGCC StyI 953 CCATGG C^C[AT][AT]GG 1206 CCTAGG Van91I 313 CCACTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 787 CCACCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AfaI AluI ApaI AvaII BalI BanI BanII BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboII MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI TseI TspRI