(Circular) (Six-base) MAPSORT of: pFN21AE6442.seq Check: 163 from: 1 to: 6070 pFN21AE6442 6070 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3880 278 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2838 3413 1937 Size: 901 575 4594 Fragments arranged by size: 4594 901 575 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3237 4133 5517 5785 1632 Size: 1605 896 1384 268 1917 Fragments arranged by size: 1917 1605 1384 896 268 AfeI AGC'GCT Cuts at: 2903 2903 Size: 6070 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5873 AflIII A'CryG_T Cuts at: 1203 4969 5946 1203 Size: 3766 977 1327 Fragments arranged by size: 3766 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 2166 1166 Size: 1000 5070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AlwNI CAG_nnn'CTG Cuts at: 2308 3337 3813 5385 2308 Size: 1029 476 1572 2993 Fragments arranged by size: 2993 1572 1029 476 ApaI G_GGCC'C Cuts at: 2579 3031 3240 3277 2579 Size: 452 209 37 5372 Fragments arranged by size: 5372 452 209 37 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 3379 3605 3757 5866 1493 Size: 1886 226 152 2109 1697 Fragments arranged by size: 2109 1886 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2144 2863 3395 1870 Size: 73 201 719 532 4545 Fragments arranged by size: 4545 719 532 201 73 AvrII C'CTAG_G Cuts at: 3211 3211 Size: 6070 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2242 2275 2242 Size: 33 6037 BamHI G'GATC_C Cuts at: 2991 3400 3767 2991 Size: 409 367 5294 Fragments arranged by size: 5294 409 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2352 2611 2698 2975 3043 Size: 325 217 627 565 259 87 277 68 Cuts at: 3043 3133 3391 4846 618 Size: 90 258 1455 1842 Fragments arranged by size: 1842 1455 627 565 325 277 259 258 217 90 87 68 BanII G_rGCy'C Cuts at: 1391 2510 2579 3031 3240 3277 3306 3389 1391 Size: 1119 69 452 209 37 29 83 4072 Fragments arranged by size: 4072 1119 452 209 83 69 37 29 BbeI G_GCGC'C Cuts at: 2356 2702 2356 Size: 346 5724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5099 BbvCI CC'TCA_GC Cuts at: 3366 3366 Size: 6070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4261 4295 1903 Size: 34 2324 34 3678 Fragments arranged by size: 3678 2324 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 1548 Size: 6070 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2435 2829 4681 Size: 107 122 71 631 605 763 394 1852 Cuts at: 4681 136 Size: 1525 Fragments arranged by size: 1852 1525 763 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 2741 2810 2882 3487 2741 Size: 69 72 605 5324 Fragments arranged by size: 5324 605 72 69 Bme1580I G_kGCm'C Cuts at: 1790 2173 2579 3031 3048 3138 3188 3240 3277 Size: 383 406 452 17 90 50 52 37 Cuts at: 3277 4131 5287 1790 Size: 854 1156 2573 Fragments arranged by size: 2573 1156 854 452 406 383 90 52 50 37 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 2570 3801 Size: 416 53 83 399 24 917 236 1231 Cuts at: 3801 4759 442 Size: 958 1753 Fragments arranged by size: 1753 1231 958 917 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6070 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2825 2825 Size: 6070 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2425 2708 2820 2891 4730 1239 Size: 1186 283 112 71 1839 2579 Fragments arranged by size: 2579 1839 1186 283 112 71 Bpu10I CC'TnA_GC Cuts at: 3366 3366 Size: 6070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3344 4195 5060 5358 3344 Size: 851 865 298 4056 Fragments arranged by size: 4056 865 851 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2279 4733 882 Size: 617 780 2454 2219 Fragments arranged by size: 2454 2219 780 617 BsaAI yAC'GTr Cuts at: 493 1204 3294 3861 493 Size: 711 2090 567 2702 Fragments arranged by size: 2702 2090 711 567 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2353 2699 3877 4259 Size: 53 83 186 936 820 346 1178 382 Cuts at: 4259 4894 275 Size: 635 1451 Fragments arranged by size: 1451 1178 936 820 635 382 346 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 1178 Size: 30 165 30 831 30 4984 Fragments arranged by size: 4984 831 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3047 1186 Size: 1861 4209 BseYI C'CCAG_C Cuts at: 3066 3088 5273 5769 3066 Size: 22 2185 496 3367 Fragments arranged by size: 3367 2185 496 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2014 1163 Size: 573 278 5219 Fragments arranged by size: 5219 573 278 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2167 3520 4281 5309 1540 Size: 275 186 166 1353 761 1028 2301 Fragments arranged by size: 2301 1353 1028 761 275 186 166 BsiHKAI G_wGCw'C Cuts at: 3220 4131 4216 5287 3220 Size: 911 85 1071 4003 Fragments arranged by size: 4003 1071 911 85 BsmI GAATG_Cn' Cuts at: 2288 3581 3674 2288 Size: 1293 93 4684 Fragments arranged by size: 4684 1293 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2669 2669 Size: 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2173 2510 2579 3031 3048 3138 3188 Size: 399 383 337 69 452 17 90 50 Cuts at: 3188 3220 3240 3277 3306 3389 4131 4216 5287 Size: 32 20 37 29 83 742 85 1071 Cuts at: 5287 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 452 399 383 337 90 85 83 69 50 37 32 29 20 17 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 3959 5689 3959 Size: 1730 4340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 3425 844 Size: 1811 770 3489 Fragments arranged by size: 3489 1811 770 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6070 BssSI C'ACGA_G Cuts at: 2778 4130 5142 2778 Size: 1352 1012 3706 Fragments arranged by size: 3706 1352 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6070 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 2077 1184 Size: 893 5177 BstYI r'GATC_y Cuts at: 1972 2991 3400 3767 4152 4169 5621 5707 5719 Size: 1019 409 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1019 409 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2161 2484 2958 3150 3231 513 Size: 552 939 157 323 474 192 81 3352 Fragments arranged by size: 3352 939 552 474 323 192 157 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5331 Fragments arranged by size: 5331 683 56 BtsI GCAGTG_nn' Cuts at: 3200 3586 4380 4400 3200 Size: 386 794 20 4870 Fragments arranged by size: 4870 794 386 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DraIII CAC_nnn'GTG Cuts at: 3294 3294 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2164 2385 2487 Size: 54 1425 50 275 18 334 221 102 Cuts at: 2487 2782 2961 3222 3246 3267 3517 4406 6035 Size: 295 179 261 24 21 250 889 1629 Cuts at: 6035 8 Size: 43 Fragments arranged by size: 1629 1425 889 334 295 275 261 250 221 179 102 54 50 43 24 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2164 3517 1537 Size: 275 352 1353 4090 Fragments arranged by size: 4090 1353 352 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 2636 2858 4000 1353 Size: 887 396 222 1142 3423 Fragments arranged by size: 3423 1142 887 396 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3004 4657 5031 5177 387 Size: 1064 138 1415 1653 374 146 1280 Fragments arranged by size: 1653 1415 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2425 2708 2820 2891 3237 4133 4730 Size: 393 793 283 112 71 346 896 597 Cuts at: 4730 5517 5785 1239 Size: 787 268 1524 Fragments arranged by size: 1524 896 793 787 597 393 346 283 268 112 71 EcoNI CCTnn'n_nnAGG Cuts at: 2924 2924 Size: 6070 EcoO109I rG'GnC_Cy Cuts at: 2576 2788 3028 3236 3237 3273 2576 Size: 212 240 208 1 36 5373 Fragments arranged by size: 5373 240 212 208 36 1 EcoRI G'AATT_C Cuts at: 3379 3757 3379 Size: 378 5692 FspI TGC'GCA Cuts at: 3206 4576 3206 Size: 1370 4700 HaeII r_GCGC'y Cuts at: 1678 2071 2356 2702 2905 5217 1678 Size: 393 285 346 203 2312 2531 Fragments arranged by size: 2531 2312 393 346 285 203 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 2780 2812 4711 Size: 32 135 32 920 32 256 32 1899 Cuts at: 4711 4743 4785 4817 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1899 920 256 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KasI G'GCGC_C Cuts at: 2352 2698 2352 Size: 346 5724 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 2667 5184 5368 1305 Size: 828 534 2517 184 2007 Fragments arranged by size: 2517 2007 828 534 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 2784 2963 3224 10 Size: 54 1425 343 555 397 179 261 2856 Fragments arranged by size: 2856 1425 555 397 343 261 179 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 2231 2546 3191 4028 4387 4546 Size: 648 1000 65 315 645 837 359 159 Cuts at: 4546 518 Size: 2042 Fragments arranged by size: 2042 1000 837 648 645 359 315 159 65 MspA1I CmG'CkG Cuts at: 1606 3233 3486 4163 5311 5556 1606 Size: 1627 253 677 1148 245 2120 Fragments arranged by size: 2120 1627 1148 677 253 245 NarI GG'CG_CC Cuts at: 2353 2699 2353 Size: 346 5724 NcoI C'CATG_G Cuts at: 513 1065 2004 2958 513 Size: 552 939 954 3625 Fragments arranged by size: 3625 954 939 552 NdeI CA'TA_TG Cuts at: 387 2395 387 Size: 2008 4062 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6070 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 3428 4973 1132 Size: 97 2199 1545 2229 Fragments arranged by size: 2229 2199 1545 97 PasI CC'CwG_GG Cuts at: 1348 2572 2713 1348 Size: 1224 141 4705 Fragments arranged by size: 4705 1224 141 PciI A'CATG_T Cuts at: 4969 4969 Size: 6070 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2324 2798 1108 Size: 902 314 474 4380 Fragments arranged by size: 4380 902 474 314 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PmlI CAC'GTG Cuts at: 3294 3294 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2788 2788 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6070 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PspOMI G'GGCC_C Cuts at: 2575 3027 3236 3273 2575 Size: 452 209 37 5372 Fragments arranged by size: 5372 452 209 37 PstI C_TGCA'G Cuts at: 830 2304 3333 3422 830 Size: 1474 1029 89 3478 Fragments arranged by size: 3478 1474 1029 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 SacII CC_GC'GG Cuts at: 3234 3234 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SapI GCTCTTCn'nnn_ Cuts at: 2636 2636 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SfcI C'TryA_G Cuts at: 826 1045 2300 2921 3329 3418 4553 5234 5425 Size: 219 1255 621 408 89 1135 681 191 Cuts at: 5425 826 Size: 1471 Fragments arranged by size: 1471 1255 1135 681 621 408 219 191 89 SfiI GGCCn_nnn'nGGCC Cuts at: 2435 2435 Size: 6070 SfoI GGC'GCC Cuts at: 2354 2700 2354 Size: 346 5724 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 2146 3397 1872 Size: 274 1251 4545 Fragments arranged by size: 4545 1251 274 SmlI C'TyrA_G Cuts at: 820 1017 1943 3359 4174 5075 5337 820 Size: 197 926 1416 815 901 262 1553 Fragments arranged by size: 1553 1416 926 901 815 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SrfI GCCC'GGGC Cuts at: 2146 2146 Size: 6070 SspI AAT'ATT Cuts at: 5 52 3994 5 Size: 47 3942 2081 Fragments arranged by size: 3942 2081 47 StuI AGG'CCT Cuts at: 1517 3281 1517 Size: 1764 4306 StyI C'CwwG_G Cuts at: 513 1065 2004 2958 3211 3509 513 Size: 552 939 954 253 298 3074 Fragments arranged by size: 3074 954 939 552 298 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2059 2369 4097 4114 4267 4452 4897 Size: 1255 479 310 1728 17 153 185 445 Cuts at: 4897 325 Size: 1498 Fragments arranged by size: 1728 1498 1255 479 445 310 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2874 3258 4316 Size: 274 80 33 51 1443 897 384 1058 Cuts at: 4316 6028 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1058 897 384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6030 6062 6030 Size: 32 6038 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 2144 3395 1870 Size: 274 1251 4545 Fragments arranged by size: 4545 1251 274 XmnI GAAnn'nnTTC Cuts at: 4199 4199 Size: 6070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3878 276 Size: 53 83 186 936 2344 2468 Fragments arranged by size: 2468 2344 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SanDI SexAI SwaI XcmI