(Linear) (Six-base) MAPSORT of: KIEE6442.seq Check: 7723 from: 1 to: 1365 KIEE6442.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AccI GT'mk_AC Cuts at: 0 833 1365 Size: 833 532 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1232 1365 Size: 1232 133 AfeI AGC'GCT Cuts at: 0 898 1365 Size: 898 467 AleI CACnn'nnGTG Cuts at: 0 161 1365 Size: 161 1204 AlwNI CAG_nnn'CTG Cuts at: 0 303 1332 1365 Size: 303 1029 33 Fragments arranged by size: 1029 303 33 ApaI G_GGCC'C Cuts at: 0 574 1026 1235 1272 1365 Size: 574 452 209 37 93 Fragments arranged by size: 574 452 209 93 37 AvaI C'yCGr_G Cuts at: 0 139 858 1365 Size: 139 719 507 Fragments arranged by size: 719 507 139 AvrII C'CTAG_G Cuts at: 0 1206 1365 Size: 1206 159 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 237 270 1365 Size: 237 33 1095 Fragments arranged by size: 1095 237 33 BamHI G'GATC_C Cuts at: 0 986 1365 Size: 986 379 BanI G'GyrC_C Cuts at: 0 347 606 693 970 1038 1128 1365 Size: 347 259 87 277 68 90 237 Fragments arranged by size: 347 277 259 237 90 87 68 BanII G_rGCy'C Cuts at: 0 505 574 1026 1235 1272 1301 1365 Size: 505 69 452 209 37 29 64 Fragments arranged by size: 505 452 209 69 64 37 29 BbeI G_GCGC'C Cuts at: 0 351 697 1365 Size: 351 346 668 Fragments arranged by size: 668 351 346 BglI GCCn_nnn'nGGC Cuts at: 0 430 824 1365 Size: 430 394 541 Fragments arranged by size: 541 430 394 BlpI GC'TnA_GC Cuts at: 0 736 805 877 1365 Size: 736 69 72 488 Fragments arranged by size: 736 488 72 69 Bme1580I G_kGCm'C Cuts at: 0 168 574 1026 1043 1133 1183 1235 1272 Size: 168 406 452 17 90 50 52 37 Cuts at: 1272 1365 Size: 93 Fragments arranged by size: 452 406 168 93 90 52 50 37 17 BmrI ACTGGGnnnn_n' Cuts at: 0 329 565 1365 Size: 329 236 800 Fragments arranged by size: 800 329 236 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 820 1365 Size: 820 545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 703 815 886 1365 Size: 420 283 112 71 479 Fragments arranged by size: 479 420 283 112 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1339 1365 Size: 1339 26 BsaI GGTCTCn'nnnn_ Cuts at: 0 274 1365 Size: 274 1091 BsaAI yAC'GTr Cuts at: 0 1289 1365 Size: 1289 76 BsaHI Gr'CG_yC Cuts at: 0 348 694 1365 Size: 348 346 671 Fragments arranged by size: 671 348 346 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 229 259 1365 Size: 229 30 1106 Fragments arranged by size: 1106 229 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1042 1365 Size: 1042 323 BseYI C'CCAG_C Cuts at: 0 1061 1083 1365 Size: 1061 22 282 Fragments arranged by size: 1061 282 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 9 1365 Size: 9 1356 BsiEI CG_ry'CG Cuts at: 0 162 1365 Size: 162 1203 BsiHKAI G_wGCw'C Cuts at: 0 1215 1365 Size: 1215 150 BsmI GAATG_Cn' Cuts at: 0 283 1365 Size: 283 1082 BsmBI CGTCTCn'nnnn_ Cuts at: 0 664 1365 Size: 664 701 Bsp1286I G_dGCh'C Cuts at: 0 168 505 574 1026 1043 1133 1183 1215 Size: 168 337 69 452 17 90 50 32 Cuts at: 1215 1235 1272 1301 1365 Size: 20 37 29 64 Fragments arranged by size: 452 337 168 90 69 64 50 37 32 29 20 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 650 1365 Size: 650 715 BssSI C'ACGA_G Cuts at: 0 773 1365 Size: 773 592 BstEII G'GTnAC_C Cuts at: 0 72 1365 Size: 72 1293 BstYI r'GATC_y Cuts at: 0 986 1365 Size: 986 379 BtgI C'CryG_G Cuts at: 0 156 479 953 1145 1226 1365 Size: 156 323 474 192 81 139 Fragments arranged by size: 474 323 192 156 139 81 BtsI GCAGTG_nn' Cuts at: 0 1195 1365 Size: 1195 170 DraIII CAC_nnn'GTG Cuts at: 0 1289 1365 Size: 1289 76 EaeI y'GGCC_r Cuts at: 0 159 380 482 777 956 1217 1241 1262 Size: 159 221 102 295 179 261 24 21 Cuts at: 1262 1365 Size: 103 Fragments arranged by size: 295 261 221 179 159 103 102 24 21 EagI C'GGCC_G Cuts at: 0 159 1365 Size: 159 1206 EarI CTCTTCn'nnn_ Cuts at: 0 235 631 853 1365 Size: 235 396 222 512 Fragments arranged by size: 512 396 235 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 999 1365 Size: 999 366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 703 815 886 1232 1365 Size: 420 283 112 71 346 133 Fragments arranged by size: 420 346 283 133 112 71 EcoNI CCTnn'n_nnAGG Cuts at: 0 919 1365 Size: 919 446 EcoO109I rG'GnC_Cy Cuts at: 0 571 783 1023 1231 1232 1268 1365 Size: 571 212 240 208 1 36 97 Fragments arranged by size: 571 240 212 208 97 36 1 FspI TGC'GCA Cuts at: 0 1201 1365 Size: 1201 164 HaeII r_GCGC'y Cuts at: 0 66 351 697 900 1365 Size: 66 285 346 203 465 Fragments arranged by size: 465 346 285 203 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 487 519 775 807 1365 Size: 487 32 256 32 558 Fragments arranged by size: 558 487 256 32 32 KasI G'GCGC_C Cuts at: 0 347 693 1365 Size: 347 346 672 Fragments arranged by size: 672 347 346 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 128 662 1365 Size: 128 534 703 Fragments arranged by size: 703 534 128 MscI TGG'CCA Cuts at: 0 382 779 958 1219 1365 Size: 382 397 179 261 146 Fragments arranged by size: 397 382 261 179 146 MslI CAynn'nnrTG Cuts at: 0 161 226 541 1186 1365 Size: 161 65 315 645 179 Fragments arranged by size: 645 315 179 161 65 MspA1I CmG'CkG Cuts at: 0 1228 1365 Size: 1228 137 NarI GG'CG_CC Cuts at: 0 348 694 1365 Size: 348 346 671 Fragments arranged by size: 671 348 346 NcoI C'CATG_G Cuts at: 0 953 1365 Size: 953 412 NdeI CA'TA_TG Cuts at: 0 390 1365 Size: 390 975 PasI CC'CwG_GG Cuts at: 0 567 708 1365 Size: 567 141 657 Fragments arranged by size: 657 567 141 PflMI CCAn_nnn'nTGG Cuts at: 0 319 793 1365 Size: 319 474 572 Fragments arranged by size: 572 474 319 PmlI CAC'GTG Cuts at: 0 1289 1365 Size: 1289 76 PpuMI rG'GwC_Cy Cuts at: 0 783 1365 Size: 783 582 PspOMI G'GGCC_C Cuts at: 0 570 1022 1231 1268 1365 Size: 570 452 209 37 97 Fragments arranged by size: 570 452 209 97 37 PstI C_TGCA'G Cuts at: 0 299 1328 1365 Size: 299 1029 37 Fragments arranged by size: 1029 299 37 SacII CC_GC'GG Cuts at: 0 1229 1365 Size: 1229 136 SapI GCTCTTCn'nnn_ Cuts at: 0 631 1365 Size: 631 734 SfcI C'TryA_G Cuts at: 0 295 916 1324 1365 Size: 295 621 408 41 Fragments arranged by size: 621 408 295 41 SfiI GGCCn_nnn'nGGCC Cuts at: 0 430 1365 Size: 430 935 SfoI GGC'GCC Cuts at: 0 349 695 1365 Size: 349 346 670 Fragments arranged by size: 670 349 346 SmaI CCC'GGG Cuts at: 0 141 1365 Size: 141 1224 SmlI C'TyrA_G Cuts at: 0 1354 1365 Size: 1354 11 SrfI GCCC'GGGC Cuts at: 0 141 1365 Size: 141 1224 StuI AGG'CCT Cuts at: 0 1276 1365 Size: 1276 89 StyI C'CwwG_G Cuts at: 0 953 1206 1365 Size: 953 253 159 Fragments arranged by size: 953 253 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 54 364 1365 Size: 54 310 1001 Fragments arranged by size: 1001 310 54 TatI w'GTAC_w Cuts at: 0 869 1253 1365 Size: 869 384 112 Fragments arranged by size: 869 384 112 XmaI C'CCGG_G Cuts at: 0 139 1365 Size: 139 1226 Enzymes that do cut: AccI AcuI AfeI AleI AlwNI ApaI AvaI AvrII BaeI BamHI BanI BanII BbeI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BssSI BstEII BstYI BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NdeI PasI PflMI PmlI PpuMI PspOMI PstI SacII SapI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BsaBI BsaWI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI