################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccII AccIII AclI AgeI Alw44I AocI Aor51HI ApaLI AscI AspI AvaI AviII AvrII BalI BbrPI BbsI BcgI BciVI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspEI BspHI BspMI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CpoI DraI DraIII DrdI DsaI EarI Eco47III Eco81I EcoO65I EcoRI EcoT22I FspI HgaI HincII HindII HindIII HpaI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI MvnI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 212 GGTACC G^GTACC AcsI 426 GAATTT [GA]^AATT[CT] AcyI 42 GGCGCC G[GA]^CG[CT]C AflII 75 CTTAAG C^TTAAG AflIII 1133 ACATGT A^C[GA][CT]GT AhdI 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 426 GAATTT [GA]^AATT[CT] AseI 730 ATTAAT AT^TAAT AsnI 730 ATTAAT AT^TAAT Asp700I 975 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 212 GGTACC G^GTACC AspEI 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 593 GAGCTC G[AT]GC[AT]^C BamHI 1395 GGATCC G^GATCC BbeI 42 GGCGCC GGCGC^C BbvCI 328 GCTGAGG CCTCAGC(-5/-2) BclI 171 TGATCA T^GATCA BcnI 123 CCCGG CC^[GC]GG BfrI 75 CTTAAG C^TTAAG BglII 1371 AGATCT A^GATCT BpmI 773 CTGGAG CTGGAG(16/14) BsaHI 42 GGCGCC G[GA]^CG[CT]C BsaI 1200 GAGACC GGTCTC(1/5) BsgI 1089 CTGCAC GTGCAG(16/14) BsiEI 67 CGGCCG CG[GA][CT]^CG BsiHKAI 593 GAGCTC G[AT]GC[AT]^C BsmFI 281 GGGAC GGGAC(10/14) BsmI 1386 GAATGC GAATGC(1/-1) BspDI 365 ATCGAT AT^CGAT BspLU11I 1133 ACATGT A^CATGT BsrBI 1419 CCGCTC CCGCTC(-3/-3) BsrFI 69 GCCGGC [GA]^CCGG[CT] BstAPI 601 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 43 GCGC GCG^C Cfr10I 69 GCCGGC [GA]^CCGG[CT] ClaI 365 ATCGAT AT^CGAT EaeI 67 CGGCCG [CT]^GGCC[GA] EagI 67 CGGCCG C^GGCCG Eam1105I 1288 GACCCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 67 CGGCCG C^GGCCG Eco52I 67 CGGCCG C^GGCCG EcoNI 557 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1318 GATATC GAT^ATC FbaI 171 TGATCA T^GATCA FseI 68 GGCCGGCC GGCCGG^CC HaeII 42 GGCGCC [GA]GCGC^[CT] HhaI 43 GCGC GCG^C Hin1I 42 GGCGCC G[GA]^CG[CT]C HinP1I 43 GCGC G^CGC KasI 42 GGCGCC G^GCGCC KpnI 212 GGTACC GGTAC^C MslI 1221 CACTACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 208 CAGCGG C[AC]G^C[GT]G NaeI 69 GCCGGC GCC^GGC NarI 42 GGCGCC GG^CGCC NciI 123 CCCGG CC^[GC]GG NgoMIV 69 GCCGGC G^CCGGC PpuMI 715 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 730 ATTAAT AT^TAAT PstI 970 CTGCAG CTGCA^G SacI 593 GAGCTC GAGCT^C SfiI 72 GGCCTTAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 42 GGCGCC GGC^GCC SmlI 75 CTTAAG C^T[CT][GA]AG SphI 545 GCATGC GCATG^C StuI 1085 AGGCCT AGG^CCT XmnI 975 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### ApaI 80 GGGCCC GGGCC^C 1358 GGGCCC AvaII 688 GGTCC G^G[AT]CC 716 GGTCC BanI 42 GGCGCC G^G[CT][GA]CC 212 GGTACC BglI 73 GCCTTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 903 GCCTGCTTGGC FauI 1418 CCCGC CCCGC(4/6) 1474 GCGGG NdeI 580 CATATG CA^TATG 1438 CATATG NspI 545 GCATGC [GA]CATG^[CT] 1133 ACATGT PleI 737 GACTC GAGTC(4/5) 379 GAGTC SfaNI 724 GATGC GCATC(5/9) 707 GCATC SspI 976 AATATT AAT^ATT 1033 AATATT Tsp45I 395 GTGAC ^GT[GC]AC 1342 GTCAC XcmI 1204 CCAAGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1382 CCAAGAATGCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BanII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII