(Circular) (Six-base) MAPSORT of: pFN21AE6419.seq Check: 1839 from: 1 to: 6193 pFN21AE6419 6193 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 2217 3514 2217 Size: 1297 4896 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4256 5640 5908 1632 Size: 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2080 820 Size: 197 1063 4933 Fragments arranged by size: 4933 1063 197 AflIII A'CryG_T Cuts at: 1203 3138 5092 6069 1203 Size: 1935 1954 977 1327 Fragments arranged by size: 1954 1935 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 3298 4922 3298 Size: 1624 4569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2780 1184 Size: 1596 4597 AlwNI CAG_nnn'CTG Cuts at: 2507 2612 2724 3936 5508 2507 Size: 105 112 1212 1572 3192 Fragments arranged by size: 3192 1572 1212 112 105 ApaI G_GGCC'C Cuts at: 2089 3367 2089 Size: 1278 4915 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2431 3502 3728 3880 5989 1493 Size: 938 1071 226 152 2109 1697 Fragments arranged by size: 2109 1697 1071 938 226 152 AseI AT'TA_AT Cuts at: 160 1032 2736 4747 160 Size: 872 1704 2011 1606 Fragments arranged by size: 2011 1704 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 3400 3523 3890 3400 Size: 123 367 5703 Fragments arranged by size: 5703 367 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 2217 3514 4969 618 Size: 325 217 627 260 170 1297 1455 1842 Fragments arranged by size: 1842 1455 1297 627 325 260 217 170 BanII G_rGCy'C Cuts at: 1391 2020 2089 2602 2785 3367 3512 1391 Size: 629 69 513 183 582 145 4072 Fragments arranged by size: 4072 629 582 513 183 145 69 BbeI G_GCGC'C Cuts at: 2051 2051 Size: 6193 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5222 BbvCI CC'TCA_GC Cuts at: 2334 2334 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4085 5295 4085 Size: 1210 4983 BclI T'GATC_A Cuts at: 1548 2176 1548 Size: 628 5565 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 2914 4804 Size: 107 122 71 631 605 412 830 1890 Cuts at: 4804 136 Size: 1525 Fragments arranged by size: 1890 1525 830 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 3376 6188 3376 Size: 2812 3381 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 2089 3367 4254 5410 1790 Size: 299 1278 887 1156 2573 Fragments arranged by size: 2573 1278 1156 887 299 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3083 3924 4882 Size: 416 53 83 399 24 1666 841 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1666 958 841 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2405 2405 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2799 4853 1239 Size: 1560 2054 2579 Fragments arranged by size: 2579 2054 1560 Bpu10I CC'TnA_GC Cuts at: 2334 2334 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4318 5183 5481 4318 Size: 865 298 5030 Fragments arranged by size: 5030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3199 4856 882 Size: 617 1700 1657 2219 Fragments arranged by size: 2219 1700 1657 617 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 4000 4382 5017 Size: 53 83 186 936 515 1952 382 635 Cuts at: 5017 275 Size: 1451 Fragments arranged by size: 1952 1451 936 635 515 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3297 3327 1178 Size: 30 165 30 1894 30 4044 Fragments arranged by size: 4044 1894 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6193 BseYI C'CCAG_C Cuts at: 5396 5892 5396 Size: 496 5697 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3079 1163 Size: 573 1343 4277 Fragments arranged by size: 4277 1343 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2075 3643 4404 5432 1540 Size: 275 186 74 1568 761 1028 2301 Fragments arranged by size: 2301 1568 1028 761 275 186 74 BsiHKAI G_wGCw'C Cuts at: 2602 4254 4339 5410 2602 Size: 1652 85 1071 3385 Fragments arranged by size: 3385 1652 1071 85 BsmI GAATG_Cn' Cuts at: 3397 3704 3797 3397 Size: 307 93 5793 Fragments arranged by size: 5793 307 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2089 2602 2785 3367 3512 4254 Size: 399 230 69 513 183 582 145 742 Cuts at: 4254 4339 5410 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 513 399 230 183 145 85 69 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3426 4080 1394 Size: 429 1603 654 3507 Fragments arranged by size: 3507 1603 654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 2871 3286 4688 4862 65 Size: 1183 1275 348 415 1402 174 1396 Fragments arranged by size: 1402 1396 1275 1183 415 348 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 4837 5024 1700 Size: 110 264 2763 187 2869 Fragments arranged by size: 2869 2763 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2612 794 Size: 1818 4375 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstYI r'GATC_y Cuts at: 1972 2838 3376 3400 3523 3890 4275 4292 5744 Size: 866 538 24 123 367 385 17 1452 Cuts at: 5744 5830 5842 6188 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 866 538 385 367 346 123 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2764 2774 2966 3709 4503 4523 2764 Size: 10 192 743 794 20 4434 Fragments arranged by size: 4434 794 743 192 20 10 ClaI AT'CG_AT Cuts at: 1554 2371 1554 Size: 817 5376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5200 809 Size: 507 3884 1802 Fragments arranged by size: 3884 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 3640 4529 Size: 54 1425 50 275 18 242 1568 889 Cuts at: 4529 6158 8 Size: 1629 43 Fragments arranged by size: 1629 1568 1425 889 275 242 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2072 3640 1537 Size: 275 260 1568 4090 Fragments arranged by size: 4090 1568 275 260 EarI CTCTTCn'nnn_ Cuts at: 1353 4123 1353 Size: 2770 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2600 2600 Size: 6193 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2799 4256 4853 5640 5908 1239 Size: 393 1167 1457 597 787 268 1524 Fragments arranged by size: 1524 1457 1167 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2566 2566 Size: 6193 EcoO109I rG'GnC_Cy Cuts at: 2085 2086 2721 3213 3363 2085 Size: 1 635 492 150 4915 Fragments arranged by size: 4915 635 492 150 1 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 EcoRV GAT'ATC Cuts at: 3325 3325 Size: 6193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3412 3412 Size: 6193 FseI GG_CCGG'CC Cuts at: 2078 2078 Size: 6193 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 2051 5340 1678 Size: 373 3289 2531 Fragments arranged by size: 3289 2531 373 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2563 2595 2825 2857 3284 Size: 32 135 32 991 32 230 32 427 Cuts at: 3284 3316 3392 3424 4834 4866 4908 4940 1373 Size: 32 76 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1410 991 427 230 135 76 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KasI G'GCGC_C Cuts at: 2047 2047 Size: 6193 KpnI G_GTAC'C Cuts at: 2221 3518 2221 Size: 1297 4896 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2188 2575 2845 3059 5307 5491 1305 Size: 883 387 270 214 2248 184 2007 Fragments arranged by size: 2248 2007 883 387 270 214 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3230 4151 4510 4669 518 Size: 648 2064 921 359 159 2042 Fragments arranged by size: 2064 2042 921 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2215 3609 4286 5434 5679 1606 Size: 609 1394 677 1148 245 2120 Fragments arranged by size: 2120 1394 1148 677 609 245 NaeI GCC'GGC Cuts at: 2076 2076 Size: 6193 NarI GG'CG_CC Cuts at: 2048 2048 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4702 Fragments arranged by size: 4702 939 552 NdeI CA'TA_TG Cuts at: 387 2586 3444 387 Size: 2199 858 3136 Fragments arranged by size: 3136 2199 858 NgoMIV G'CCGG_C Cuts at: 2074 2074 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 2554 3142 3551 5096 1132 Size: 97 1325 588 409 1545 2229 Fragments arranged by size: 2229 1545 1325 588 409 97 PasI CC'CwG_GG Cuts at: 1348 2052 1348 Size: 704 5489 PciI A'CATG_T Cuts at: 3138 5092 3138 Size: 1954 4239 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PfoI T'CCnGG_A Cuts at: 3218 3218 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2721 2721 Size: 6193 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2085 3363 2085 Size: 1278 4915 PstI C_TGCA'G Cuts at: 830 2979 3545 830 Size: 2149 566 3478 Fragments arranged by size: 3478 2149 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 SacI G_AGCT'C Cuts at: 2602 2602 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2133 2673 2975 3541 4676 5357 5548 Size: 219 1088 540 302 566 1135 681 191 Cuts at: 5548 826 Size: 1471 Fragments arranged by size: 1471 1135 1088 681 566 540 302 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2084 2084 Size: 6193 SfoI GGC'GCC Cuts at: 2049 2049 Size: 6193 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 4297 5198 5460 820 Size: 197 926 137 2217 901 262 1553 Fragments arranged by size: 2217 1553 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 2554 3551 1132 Size: 1422 997 3774 Fragments arranged by size: 3774 1422 997 SspI AAT'ATT Cuts at: 5 52 2983 3040 4117 5 Size: 47 2931 57 1077 2081 Fragments arranged by size: 2931 2081 1077 57 47 StuI AGG'CCT Cuts at: 1517 3092 1517 Size: 1575 4618 StyI C'CwwG_G Cuts at: 513 1065 2004 3209 3396 3429 3632 513 Size: 552 939 1205 187 33 203 3074 Fragments arranged by size: 3074 1205 939 552 203 187 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2842 4220 4237 4390 4575 5020 325 Size: 1255 1262 1378 17 153 185 445 1498 Fragments arranged by size: 1498 1378 1262 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3119 4439 6151 Size: 274 80 33 51 1443 1142 1320 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 1712 1443 1320 1142 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3216 3394 3216 Size: 178 6015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 2984 4322 2984 Size: 1338 4855 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SrfI SwaI