(Linear) (Six-base) MAPSORT of: KIEE6419.seq Check: 1984 from: 1 to: 1488 KIEE6419.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. Acc65I G'GTAC_C Cuts at: 0 212 1488 Size: 212 1276 AflII C'TTAA_G Cuts at: 0 75 1488 Size: 75 1413 AflIII A'CryG_T Cuts at: 0 1133 1488 Size: 1133 355 AhdI GACnn_n'nnGTC Cuts at: 0 1293 1488 Size: 1293 195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 775 1488 Size: 775 713 AlwNI CAG_nnn'CTG Cuts at: 0 502 607 719 1488 Size: 502 105 112 769 Fragments arranged by size: 769 502 112 105 ApaI G_GGCC'C Cuts at: 0 84 1362 1488 Size: 84 1278 126 Fragments arranged by size: 1278 126 84 ApoI r'AATT_y Cuts at: 0 426 1488 Size: 426 1062 AseI AT'TA_AT Cuts at: 0 731 1488 Size: 731 757 BamHI G'GATC_C Cuts at: 0 1395 1488 Size: 1395 93 BanI G'GyrC_C Cuts at: 0 42 212 1488 Size: 42 170 1276 Fragments arranged by size: 1276 170 42 BanII G_rGCy'C Cuts at: 0 15 84 597 780 1362 1488 Size: 15 69 513 183 582 126 Fragments arranged by size: 582 513 183 126 69 15 BbeI G_GCGC'C Cuts at: 0 46 1488 Size: 46 1442 BbvCI CC'TCA_GC Cuts at: 0 329 1488 Size: 329 1159 BclI T'GATC_A Cuts at: 0 171 1488 Size: 171 1317 BglI GCCn_nnn'nGGC Cuts at: 0 79 909 1488 Size: 79 830 579 Fragments arranged by size: 830 579 79 BglII A'GATC_T Cuts at: 0 1371 1488 Size: 1371 117 Bme1580I G_kGCm'C Cuts at: 0 84 1362 1488 Size: 84 1278 126 Fragments arranged by size: 1278 126 84 BmrI ACTGGGnnnn_n' Cuts at: 0 1078 1488 Size: 1078 410 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 400 1488 Size: 400 1088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 1488 Size: 794 694 Bpu10I CC'TnA_GC Cuts at: 0 329 1488 Size: 329 1159 BsaI GGTCTCn'nnnn_ Cuts at: 0 1194 1488 Size: 1194 294 BsaHI Gr'CG_yC Cuts at: 0 43 1488 Size: 43 1445 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1292 1322 1488 Size: 1292 30 166 Fragments arranged by size: 1292 166 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1074 1488 Size: 1074 414 BsiEI CG_ry'CG Cuts at: 0 70 1488 Size: 70 1418 BsiHKAI G_wGCw'C Cuts at: 0 597 1488 Size: 597 891 BsmI GAATG_Cn' Cuts at: 0 1392 1488 Size: 1392 96 Bsp1286I G_dGCh'C Cuts at: 0 15 84 597 780 1362 1488 Size: 15 69 513 183 582 126 Fragments arranged by size: 582 513 183 126 69 15 BsrBI CCG'CTC Cuts at: 0 1421 1488 Size: 1421 67 BsrDI GCAATG_nn' Cuts at: 0 518 866 1281 1488 Size: 518 348 415 207 Fragments arranged by size: 518 415 348 207 BsrFI r'CCGG_y Cuts at: 0 69 1488 Size: 69 1419 BstAPI GCAn_nnn'nTGC Cuts at: 0 607 1488 Size: 607 881 BstYI r'GATC_y Cuts at: 0 833 1371 1395 1488 Size: 833 538 24 93 Fragments arranged by size: 833 538 93 24 BtsI GCAGTG_nn' Cuts at: 0 759 769 961 1488 Size: 759 10 192 527 Fragments arranged by size: 759 527 192 10 ClaI AT'CG_AT Cuts at: 0 366 1488 Size: 366 1122 EaeI y'GGCC_r Cuts at: 0 67 1488 Size: 67 1421 EagI C'GGCC_G Cuts at: 0 67 1488 Size: 67 1421 EcoICRI GAG'CTC Cuts at: 0 595 1488 Size: 595 893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 794 1488 Size: 794 694 EcoNI CCTnn'n_nnAGG Cuts at: 0 561 1488 Size: 561 927 EcoO109I rG'GnC_Cy Cuts at: 0 80 81 716 1208 1358 1488 Size: 80 1 635 492 150 130 Fragments arranged by size: 635 492 150 130 80 1 EcoRV GAT'ATC Cuts at: 0 1320 1488 Size: 1320 168 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1407 1488 Size: 1407 81 FseI GG_CCGG'CC Cuts at: 0 73 1488 Size: 73 1415 HaeII r_GCGC'y Cuts at: 0 46 1488 Size: 46 1442 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 558 590 820 852 1279 1311 1387 1419 Size: 558 32 230 32 427 32 76 32 Cuts at: 1419 1488 Size: 69 Fragments arranged by size: 558 427 230 76 69 32 32 32 32 KasI G'GCGC_C Cuts at: 0 42 1488 Size: 42 1446 KpnI G_GTAC'C Cuts at: 0 216 1488 Size: 216 1272 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 183 570 840 1054 1488 Size: 183 387 270 214 434 Fragments arranged by size: 434 387 270 214 183 MslI CAynn'nnrTG Cuts at: 0 1225 1488 Size: 1225 263 MspA1I CmG'CkG Cuts at: 0 210 1488 Size: 210 1278 NaeI GCC'GGC Cuts at: 0 71 1488 Size: 71 1417 NarI GG'CG_CC Cuts at: 0 43 1488 Size: 43 1445 NdeI CA'TA_TG Cuts at: 0 581 1439 1488 Size: 581 858 49 Fragments arranged by size: 858 581 49 NgoMIV G'CCGG_C Cuts at: 0 69 1488 Size: 69 1419 NspI r_CATG'y Cuts at: 0 549 1137 1488 Size: 549 588 351 Fragments arranged by size: 588 549 351 PasI CC'CwG_GG Cuts at: 0 47 1488 Size: 47 1441 PciI A'CATG_T Cuts at: 0 1133 1488 Size: 1133 355 PfoI T'CCnGG_A Cuts at: 0 1213 1488 Size: 1213 275 PpuMI rG'GwC_Cy Cuts at: 0 716 1488 Size: 716 772 PspOMI G'GGCC_C Cuts at: 0 80 1358 1488 Size: 80 1278 130 Fragments arranged by size: 1278 130 80 PstI C_TGCA'G Cuts at: 0 974 1488 Size: 974 514 SacI G_AGCT'C Cuts at: 0 597 1488 Size: 597 891 SfcI C'TryA_G Cuts at: 0 128 668 970 1488 Size: 128 540 302 518 Fragments arranged by size: 540 518 302 128 SfiI GGCCn_nnn'nGGCC Cuts at: 0 79 1488 Size: 79 1409 SfoI GGC'GCC Cuts at: 0 44 1488 Size: 44 1444 SmlI C'TyrA_G Cuts at: 0 75 1488 Size: 75 1413 SphI G_CATG'C Cuts at: 0 549 1488 Size: 549 939 SspI AAT'ATT Cuts at: 0 978 1035 1488 Size: 978 57 453 Fragments arranged by size: 978 453 57 StuI AGG'CCT Cuts at: 0 1087 1488 Size: 1087 401 StyI C'CwwG_G Cuts at: 0 1204 1391 1424 1488 Size: 1204 187 33 64 Fragments arranged by size: 1204 187 64 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 837 1488 Size: 837 651 TatI w'GTAC_w Cuts at: 0 1114 1488 Size: 1114 374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1211 1389 1488 Size: 1211 178 99 Fragments arranged by size: 1211 178 99 XmnI GAAnn'nnTTC Cuts at: 0 979 1488 Size: 979 509 Enzymes that do cut: Acc65I AflII AflIII AhdI AlfI AlwNI ApaI ApoI AseI BamHI BanI BanII BbeI BbvCI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstYI BtsI ClaI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I KasI KpnI MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI PfoI PpuMI PspOMI PstI SacI SfcI SfiI SfoI SmlI SphI SspI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AgeI AleI AloI ApaLI AscI AsiSI AvaI AvrII BaeI BbsI BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EarI EciI EcoRI FspI FspAI HincII HindIII HpaI MfeI MluI MscI NcoI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI