################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcnI BfaI BfrI BglI BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MroI MslI MspA1I MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 468 GTCTAC GT^[AC][GT]AC AccII 1145 CGCG CG^CG AcsI 68 GAATTC [GA]^AATT[CT] Alw44I 761 GTGCAC G^TGCAC AlwNI 899 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 761 GTGCAC G^TGCAC ApoI 68 GAATTC [GA]^AATT[CT] BanI 894 GGCACC G^G[CT][GA]CC BanII 692 GAGCTC G[GA]GC[CT]^C BcgI 621 CGACAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 245 GGATAC GTATCC(6/5) BclI 567 TGATCA T^GATCA BglII 189 AGATCT A^GATCT BlpI 937 GCTCAGC GC^T[ACGT]AGC BpmI 377 CTGGAG CTGGAG(16/14) Bpu1102I 937 GCTCAGC GC^T[ACGT]AGC BsaBI 184 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspMI 706 GCAGGT ACCTGC(4/8) BsrDI 627 CATTGC GCAATG(2/0) BsrFI 751 GCCGGC [GA]^CCGG[CT] BstBI 335 TTCGAA TT^CGAA BstF5I 423 GGATG GGATG(2/0) BstUI 1145 CGCG CG^CG BstXI 864 CCAGGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 937 GCTCAGC GC^T[ACGT]AGC CfoI 1144 GCGC GCG^C Cfr10I 751 GCCGGC [GA]^CCGG[CT] DraI 510 TTTAAA TTT^AAA DraIII 584 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 68 GAATTC G^AATTC FbaI 567 TGATCA T^GATCA FokI 423 GGATG GGATG(9/13) HapII 752 CCGG C^CGG HhaI 1144 GCGC GCG^C HinP1I 1144 GCGC G^CGC HpaII 752 CCGG C^CGG MamI 184 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 752 CCGG C^CGG MvnI 1145 CGCG CG^CG NaeI 751 GCCGGC GCC^GGC NgoMIV 751 GCCGGC G^CCGGC NlaIII 1110 CATG CATG^ NspV 335 TTCGAA TT^CGAA PleI 699 GACTC GAGTC(4/5) PpuMI 387 AGGACCT [GA]G^G[AT]CC[CT] PstI 596 CTGCAG CTGCA^G SacI 692 GAGCTC GAGCT^C SfaNI 117 GATGC GCATC(5/9) SfuI 335 TTCGAA TT^CGAA SmlI 701 CTCAAG C^T[CT][GA]AG SspI 400 AATATT AAT^ATT XcmI 1179 CCAACCTGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 772 CCGC CCGC(-3/-1) 943 CCGC AlwI 505 GATCC GGATC(4/5) 15 GGATC BalI 273 TGGCCA TGG^CCA 873 TGGCCA BsgI 974 CTGCAC GTGCAG(16/14) 237 GTGCAG BsrI 838 ACTGG ACTGG(1/-1) 587 CCAGT BstYI 15 GGATCT [GA]^GATC[CT] 189 AGATCT DraII 387 AGGACCT [GA]G^G[ACGT]CC[CT] 1063 GGGGCCT EaeI 273 TGGCCA [CT]^GGCC[GA] 873 TGGCCA EcoO109I 387 AGGACCT [GA]G^G[ACGT]CC[CT] 1063 GGGGCCT HphI 303 GGTGA GGTGA(8/7) 651 TCACC MaeIII 160 GTAAC ^GT[ACGT]AC 1148 GTAAC MflI 15 GGATCT [GA]^GATC[CT] 189 AGATCT MluNI 273 TGGCCA TGG^CCA 873 TGGCCA MscI 273 TGGCCA TGG^CCA 873 TGGCCA SexAI 1080 ACCAGGT A^CC[AT]GGT 1188 ACCTGGT SfcI 596 CTGCAG C^T[GA][CT]AG 825 CTATAG XhoII 15 GGATCT [GA]^GATC[CT] 189 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AfaI AluI AspHI AvaII BbvCI BbvI BmyI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I