Restriction map for
pFN21AE1449
(Circular) (Six-base) MAPSORT of: pFN21AE1449.seq Check: 2041 from: 1 to: 5905
pFN21AE1449 5905 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 3248 1937
Size: 537 774 4594
Fragments arranged by size:
4594 774 537
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3968 5352 5620 1632
Size: 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 4804 5781 1203
Size: 3601 977 1327
Fragments arranged by size:
3601 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2257 1184
Size: 1073 4832
AlwNI CAG_nnn'CTG
Cuts at: 2909 3648 5220 2909
Size: 739 1572 3594
Fragments arranged by size:
3594 1572 739
ApaLI G'TGCA_C
Cuts at: 2766 3962 5118 2766
Size: 1196 1156 3553
Fragments arranged by size:
3553 1196 1156
ApoI r'AATT_y
Cuts at: 1493 2073 3214 3440 3592 5701 1493
Size: 580 1141 226 152 2109 1697
Fragments arranged by size:
2109 1697 1141 580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 3230 1870
Size: 73 1287 4545
Fragments arranged by size:
4545 1287 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2942 2975 2942
Size: 33 5872
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2899 3226 4681 618
Size: 325 217 627 1112 327 1455 1842
Fragments arranged by size:
1842 1455 1112 627 327 325 217
BanII G_rGCy'C
Cuts at: 1391 2701 3224 1391
Size: 1310 523 4072
Fragments arranged by size:
4072 1310 523
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BbvCI CC'TCA_GC
Cuts at: 2647 2790 2859 2647
Size: 143 69 5693
Fragments arranged by size:
5693 143 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2615 2649 4096 4130 1903
Size: 34 678 34 1447 34 3678
Fragments arranged by size:
3678 1447 678 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2244 3797 5007 2244
Size: 1553 1210 3142
Fragments arranged by size:
3142 1553 1210
BclI T'GATC_A
Cuts at: 1548 2572 1548
Size: 1024 4881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2194 5900 2194
Size: 3706 2199
BlpI GC'TnA_GC
Cuts at: 2943 3322 2943
Size: 379 5526
Bme1580I G_kGCm'C
Cuts at: 1790 2770 3966 5122 1790
Size: 980 1196 1156 2573
Fragments arranged by size:
2573 1196 1156 980
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3636 4594
Size: 416 53 83 399 24 1169 1050 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1169 1050 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2403 4565 1239
Size: 1164 2162 2579
Fragments arranged by size:
2579 2162 1164
Bpu10I CC'TnA_GC
Cuts at: 2647 2790 2859 2647
Size: 143 69 5693
Fragments arranged by size:
5693 143 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2691 4030 4895 5193 2691
Size: 1339 865 298 3403
Fragments arranged by size:
3403 1339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 2193 5899 2193
Size: 3706 2199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3013 3043 3109 3139 1178
Size: 30 165 30 1610 30 66 30 3944
Fragments arranged by size:
3944 1610 165 66 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2632 2635 3114 1186
Size: 1446 3 479 3977
Fragments arranged by size:
3977 1446 479 3
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2263 2964 1163
Size: 573 527 701 4104
Fragments arranged by size:
4104 701 573 527
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2599 2701 2770 3966 4051 5122 2599
Size: 102 69 1196 85 1071 3382
Fragments arranged by size:
3382 1196 1071 102 85 69
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2599 2701 2770 3224 3966 4051 5122
Size: 399 809 102 69 454 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 809 742 454 399 102 85
69
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 3260 844
Size: 1858 558 3489
Fragments arranged by size:
3489 1858 558
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 4400 4574 65
Size: 1183 1383 1769 174 1396
Fragments arranged by size:
1769 1396 1383 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2756 4549 4736 1700
Size: 110 946 1793 187 2869
Fragments arranged by size:
2869 1793 946 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 2341 4709 2341
Size: 2368 3537
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2876 2876
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2020 2194 3235 3602 3987 4004 5456 5542
Size: 48 174 1041 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1041 385 367 346 174 86
48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5353
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2991 3421 4215 4235 2991
Size: 430 794 20 4661
Fragments arranged by size:
4661 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 2517 3209 2517
Size: 692 5213
DraIII CAC_nnn'GTG
Cuts at: 2594 2594
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 2878 3352
Size: 54 1425 50 275 18 448 600 474
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 600 474 448 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2699 2699
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 3968 4565 5352 5620 1239
Size: 393 771 1565 597 787 268 1524
Fragments arranged by size:
1565 1524 787 771 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2393 3069 2393
Size: 676 5229
EcoRI G'AATT_C
Cuts at: 2073 3214 3592 2073
Size: 1141 378 4386
Fragments arranged by size:
4386 1141 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2069 2069
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2710 2742 2833 2865 2959
Size: 32 135 32 1138 32 91 32 94
Cuts at: 2959 2991 3109 3141 4546 4578 4620 4652 1373
Size: 32 118 32 1405 32 42 32 2626
Fragments arranged by size:
2626 1405 1138 135 118 94 91 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 5019 5203 1305
Size: 693 3021 184 2007
Fragments arranged by size:
3021 2007 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2280 2880 10
Size: 54 1425 343 448 600 3035
Fragments arranged by size:
3035 1425 600 448 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3863 4222 4381 518
Size: 648 2697 359 159 2042
Fragments arranged by size:
2697 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3321 3998 5146 5391 1606
Size: 1715 677 1148 245 2120
Fragments arranged by size:
2120 1715 1148 677 245
NaeI GCC'GGC
Cuts at: 2758 2758
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5353
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 2756 2756
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 3103 3103
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2393 2393
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 2201 3475 2201
Size: 1274 4631
PstI C_TGCA'G
Cuts at: 830 2605 3257 830
Size: 1775 652 3478
Fragments arranged by size:
3478 1775 652
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
SacI G_AGCT'C
Cuts at: 2701 2701
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SexAI A'CCwGG_T
Cuts at: 3085 3193 3085
Size: 108 5797
SfcI C'TryA_G
Cuts at: 826 1045 2601 2830 3253 4388 5069 5260 826
Size: 219 1556 229 423 1135 681 191 1471
Fragments arranged by size:
1556 1471 1135 681 423 229 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2706 4009 4910 5172 820
Size: 197 926 763 1303 901 262 1553
Fragments arranged by size:
1553 1303 926 901 763 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 2407 3829 5
Size: 47 2355 1422 2081
Fragments arranged by size:
2355 2081 1422 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 3344 513
Size: 552 2279 3074
Fragments arranged by size:
3074 2279 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2605 2673 3932 3949 4102 4287 4732
Size: 1255 1025 68 1259 17 153 185 445
Cuts at: 4732 325
Size: 1498
Fragments arranged by size:
1498 1259 1255 1025 445 185 153 68
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2392 2424 5865 5897 2392
Size: 32 3441 32 2400
Fragments arranged by size:
3441 2400 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3191 3191
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI