################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbsI BciVI BcnI BfrI BglII BlnI BpuAI BsaAI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MboII MfeI MluI MroI MseI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 749 GGTACC G^GTACC AflIII 598 ACGTGT A^C[GA][CT]GT AocI 631 CCTGAGG CC^T[ACGT]AGG Asp718I 749 GGTACC G^GTACC AvaI 571 CTCGAG C^[CT]CG[GA]G AvaII 15 GGTCC G^G[AT]CC BalI 44 TGGCCA TGG^CCA BamHI 839 GGATCC G^GATCC BbvCI 960 GCTGAGG CCTCAGC(-5/-2) BcgI 146 GCAGTGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 584 TGATCA T^GATCA BfaI 313 CTAG C^TAG BglI 781 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1017 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1017 GCTCAGC GC^T[ACGT]AGC BsaI 742 GGTCTC GGTCTC(1/5) BsaWI 71 TCCGGT [AT]^CCGG[AT] BsiEI 73 CGGTCG CG[GA][CT]^CG BsmBI 595 GAGACG CGTCTC(1/5) BsoBI 571 CTCGAG C^[CT]CG[GA]G BspMI 174 GCAGGT ACCTGC(4/8) BsrBI 86 GAGCGG CCGCTC(-3/-3) BsrDI 402 CATTGC GCAATG(2/0) BsrFI 157 GCCGGT [GA]^CCGG[CT] BsrI 511 ACTGG ACTGG(1/-1) BstAPI 231 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 201 CCATGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 839 GGATCC [GA]^GATC[CT] Bsu36I 631 CCTGAGG CC^T[ACGT]AGG CelII 1017 GCTCAGC GC^T[ACGT]AGC Cfr10I 157 GCCGGT [GA]^CCGG[CT] DrdI 331 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1012 CCATGG C^C[GA][CT]GG EaeI 44 TGGCCA [CT]^GGCC[GA] Eco81I 631 CCTGAGG CC^T[ACGT]AGG EcoNI 378 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 584 TGATCA T^GATCA KpnI 749 GGTACC GGTAC^C MaeI 313 CTAG C^TAG MaeII 598 ACGT A^CGT MflI 839 GGATCC [GA]^GATC[CT] MluNI 44 TGGCCA TGG^CCA MscI 44 TGGCCA TGG^CCA MslI 202 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1012 CCATGG C^CATGG PaeR7I 571 CTCGAG C^TCGAG PpuMI 14 GGGTCCT [GA]G^G[AT]CC[CT] SmlI 571 CTCGAG C^T[CT][GA]AG StuI 799 AGGCCT AGG^CCT XhoI 571 CTCGAG C^TCGAG XhoII 839 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 840 GATCC GGATC(4/5) 839 GGATC AspHI 268 GAGCAC G[AT]GC[AT]^C 1024 GAGCAC BsgI 501 CTGCAC GTGCAG(16/14) 149 GTGCAG BsiHKAI 268 GAGCAC G[AT]GC[AT]^C 1024 GAGCAC BsmAI 595 GAGAC GTCTC(1/5) 743 GTCTC BsmFI 79 GGGAC GGGAC(10/14) 894 GTCCC Bsp1407I 611 TGTACA T^GTACA 809 TGTACA BsrGI 611 TGTACA T^GTACA 809 TGTACA BssSI 220 CTCGTG CACGAG(-5/-1) 869 CTCGTG DpnII 585 GATC ^GATC 840 GATC EcoT14I 335 CCAAGG C^C[AT][AT]GG 1012 CCATGG FauI 440 CCCGC CCCGC(4/6) 791 CCCGC MboI 585 GATC ^GATC 840 GATC MspA1I 31 CAGCTG C[AC]G^C[GT]G 245 CAGCTG NdeII 585 GATC ^GATC 840 GATC PstI 467 CTGCAG CTGCA^G 562 CTGCAG PvuII 31 CAGCTG CAG^CTG 245 CAGCTG Sau3AI 585 GATC ^GATC 840 GATC SfcI 467 CTGCAG C^T[GA][CT]AG 562 CTGCAG SspBI 611 TGTACA T^GTACA 809 TGTACA StyI 335 CCAAGG C^C[AT][AT]GG 1012 CCATGG TaqI 572 TCGA T^CGA 620 TCGA TthHB8I 572 TCGA T^CGA 620 TCGA XcmI 201 CCATGTGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 702 CCAGGGTGACTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AcyI AfaI AluI ApaI BanI BanII BbvI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI TseI Tsp45I TspRI