################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BspDI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MscI MseI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 654 TCCGGA T^CCGGA AcsI 658 GAATTC [GA]^AATT[CT] AlwI 591 GATCC GGATC(4/5) ApoI 658 GAATTC [GA]^AATT[CT] BbrPI 463 CACGTG CAC^GTG BciVI 81 GGATAC GTATCC(6/5) BfaI 793 CTAG C^TAG BsaAI 463 CACGTG [CT]AC^GT[GA] BsaI 109 GGTCTC GGTCTC(1/5) BsaWI 654 TCCGGA [AT]^CCGG[AT] BseAI 654 TCCGGA T^CCGGA BseRI 343 CTCCTC GAGGAG(10/8) BsmBI 688 GAGACG CGTCTC(1/5) Bsp1407I 356 TGTACA T^GTACA BspEI 654 TCCGGA T^CCGGA BspHI 717 TCATGA T^CATGA BspMI 74 ACCTGC ACCTGC(4/8) BsrBI 131 CCGCTC CCGCTC(-3/-3) BsrGI 356 TGTACA T^GTACA BstXI 297 CCAGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 591 GATC ^GATC DsaI 167 CCATGG C^C[GA][CT]GG EcoNI 663 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 658 GAATTC G^AATTC EcoT22I 682 ATGCAT ATGCA^T HgaI 690 GACGC GACGC(5/10) HinfI 943 GACTC G^A[ACGT]TC MaeI 793 CTAG C^TAG MaeII 464 ACGT A^CGT MaeIII 466 GTGAC ^GT[ACGT]AC MboI 591 GATC ^GATC MroI 654 TCCGGA T^CCGGA NcoI 167 CCATGG C^CATGG NdeII 591 GATC ^GATC NsiI 682 ATGCAT ATGCA^T NspI 680 GCATGC [GA]CATG^[CT] PflMI 231 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 943 GACTC GAGTC(4/5) PmaCI 463 CACGTG CAC^GTG PmlI 463 CACGTG CAC^GTG PstI 872 CTGCAG CTGCA^G RcaI 717 TCATGA T^CATGA SacI 957 GAGCTC GAGCT^C SapI 885 GAAGAGC GCTCTTC(1/4) Sau3AI 591 GATC ^GATC ScaI 309 AGTACT AGT^ACT SphI 680 GCATGC GCATG^C SspBI 356 TGTACA T^GTACA TaqI 545 TCGA T^CGA Tsp45I 466 GTGAC ^GT[GC]AC Tsp509I 659 AATT ^AATT TthHB8I 545 TCGA T^CGA Van91I 231 CCATCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AocI 807 CCTGAGG CC^T[ACGT]AGG 862 CCTCAGG ApaI 162 GGGCCC GGGCC^C 951 GGGCCC AspHI 316 GTGCTC G[AT]GC[AT]^C 957 GAGCTC BbsI 447 GAAGAC GAAGAC(2/6) 552 GAAGAC BglI 51 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 395 GCCTCCTGGGC BpuAI 447 GAAGAC GAAGAC(2/6) 552 GAAGAC BsgI 753 CTGCAC GTGCAG(16/14) 673 GTGCAG BsiHKAI 316 GTGCTC G[AT]GC[AT]^C 957 GAGCTC BsmAI 688 GAGAC GTCTC(1/5) 110 GTCTC BsmI 253 GCATTC GAATGC(1/-1) 488 GCATTC BstAPI 629 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 675 GCAGTGCATGC Bsu36I 807 CCTGAGG CC^T[ACGT]AGG 862 CCTCAGG CfoI 768 GCGC GCG^C 774 GCGC EaeI 494 TGGCCG [CT]^GGCC[GA] 575 TGGCCG EarI 181 GAAGAG CTCTTC(1/4) 885 GAAGAG Eco81I 807 CCTGAGG CC^T[ACGT]AGG 862 CCTCAGG HhaI 768 GCGC GCG^C 774 GCGC HinP1I 768 GCGC G^CGC 774 GCGC HphI 528 GGTGA GGTGA(8/7) 347 TCACC Ksp632I 181 GAAGAG CTCTTC(1/4) 885 GAAGAG PpuMI 811 AGGACCC [GA]G^G[AT]CC[CT] 821 GGGACCT SfcI 410 CTACAG C^T[GA][CT]AG 872 CTGCAG SmaI 54 CCCGGG CCC^GGG 897 CCCGGG SmlI 265 CTCAAG C^T[CT][GA]AG 891 CTTGAG StuI 563 AGGCCT AGG^CCT 585 AGGCCT XcmI 261 CCATCTCAAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 470 CCAGAGAGAAGCTGG XmaCI 54 CCCGGG C^CCGGG 897 CCCGGG XmaI 54 CCCGGG C^CCGGG 897 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI StyI TseI TspRI