Restriction map for
pFN21AE5407
(Linear) (Six-base) MAPSORT of: KIEE5407.seq Check: 3576 from: 1 to: 993
KIEE5407.orf
With 182 enzymes: SgfI *
January 29, 2014 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 83 993
Size: 83 910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 571 993
Size: 124 447 422
Fragments arranged by size:
447 422 124
AleI CACnn'nnGTG
Cuts at: 0 760 993
Size: 760 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 778 993
Size: 778 215
ApaI G_GGCC'C
Cuts at: 0 166 955 993
Size: 166 789 38
Fragments arranged by size:
789 166 38
ApoI r'AATT_y
Cuts at: 0 658 993
Size: 658 335
AvaI C'yCGr_G
Cuts at: 0 54 897 954 993
Size: 54 843 57 39
Fragments arranged by size:
843 57 54 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 197 230 993
Size: 197 33 763
Fragments arranged by size:
763 197 33
BanII G_rGCy'C
Cuts at: 0 166 898 955 961 993
Size: 166 732 57 6 32
Fragments arranged by size:
732 166 57 32 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 454 559 993
Size: 454 105 434
Fragments arranged by size:
454 434 105
BciVI GTATCCnnnnn_n'
Cuts at: 0 75 993
Size: 75 918
BfrBI ATG'CAT
Cuts at: 0 684 993
Size: 684 309
BglI GCCn_nnn'nGGC
Cuts at: 0 57 401 993
Size: 57 344 592
Fragments arranged by size:
592 344 57
Bme1580I G_kGCm'C
Cuts at: 0 166 804 955 993
Size: 166 638 151 38
Fragments arranged by size:
638 166 151 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 810 917 993
Size: 810 107 76
Fragments arranged by size:
810 107 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 901 993
Size: 326 575 92
Fragments arranged by size:
575 326 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 912 993
Size: 250 662 81
Fragments arranged by size:
662 250 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 115 993
Size: 115 878
BsaAI yAC'GTr
Cuts at: 0 465 993
Size: 465 528
BsaWI w'CCGG_w
Cuts at: 0 654 993
Size: 654 339
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 299 329 993
Size: 299 30 664
Fragments arranged by size:
664 299 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 993
Size: 334 659
BseYI C'CCAG_C
Cuts at: 0 159 993
Size: 159 834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 738 993
Size: 694 44 255
Fragments arranged by size:
694 255 44
BsiHKAI G_wGCw'C
Cuts at: 0 320 961 993
Size: 320 641 32
Fragments arranged by size:
641 320 32
BsmI GAATG_Cn'
Cuts at: 0 253 488 993
Size: 253 235 505
Fragments arranged by size:
505 253 235
BsmBI CGTCTCn'nnnn_
Cuts at: 0 682 993
Size: 682 311
Bsp1286I G_dGCh'C
Cuts at: 0 166 320 804 898 955 961 993
Size: 166 154 484 94 57 6 32
Fragments arranged by size:
484 166 154 94 57 32 6
BspEI T'CCGG_A
Cuts at: 0 654 993
Size: 654 339
BspHI T'CATG_A
Cuts at: 0 717 993
Size: 717 276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 993
Size: 83 910
BsrBI CCG'CTC
Cuts at: 0 133 993
Size: 133 860
BsrGI T'GTAC_A
Cuts at: 0 356 993
Size: 356 637
BstAPI GCAn_nnn'nTGC
Cuts at: 0 635 681 993
Size: 635 46 312
Fragments arranged by size:
635 312 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 993
Size: 304 689
Bsu36I CC'TnA_GG
Cuts at: 0 808 863 993
Size: 808 55 130
Fragments arranged by size:
808 130 55
BtgI C'CryG_G
Cuts at: 0 167 993
Size: 167 826
BtsI GCAGTG_nn'
Cuts at: 0 682 743 993
Size: 682 61 250
Fragments arranged by size:
682 250 61
EaeI y'GGCC_r
Cuts at: 0 494 575 993
Size: 494 81 418
Fragments arranged by size:
494 418 81
EarI CTCTTCn'nnn_
Cuts at: 0 176 880 993
Size: 176 704 113
Fragments arranged by size:
704 176 113
EcoICRI GAG'CTC
Cuts at: 0 959 993
Size: 959 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 326 571 901 993
Size: 124 202 245 330 92
Fragments arranged by size:
330 245 202 124 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 993
Size: 667 326
EcoO109I rG'GnC_Cy
Cuts at: 0 163 522 812 822 951 993
Size: 163 359 290 10 129 42
Fragments arranged by size:
359 290 163 129 42 10
EcoRI G'AATT_C
Cuts at: 0 658 993
Size: 658 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 326 358 575 607 993
Size: 326 32 217 32 386
Fragments arranged by size:
386 326 217 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 563 993
Size: 269 294 430
Fragments arranged by size:
430 294 269
MslI CAynn'nnrTG
Cuts at: 0 240 352 760 993
Size: 240 112 408 233
Fragments arranged by size:
408 240 233 112
NcoI C'CATG_G
Cuts at: 0 167 993
Size: 167 826
NsiI A_TGCA'T
Cuts at: 0 686 993
Size: 686 307
NspI r_CATG'y
Cuts at: 0 684 993
Size: 684 309
PasI CC'CwG_GG
Cuts at: 0 948 993
Size: 948 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 993
Size: 237 756
PfoI T'CCnGG_A
Cuts at: 0 836 927 993
Size: 836 91 66
Fragments arranged by size:
836 91 66
PmlI CAC'GTG
Cuts at: 0 465 993
Size: 465 528
PpuMI rG'GwC_Cy
Cuts at: 0 812 822 993
Size: 812 10 171
Fragments arranged by size:
812 171 10
PspOMI G'GGCC_C
Cuts at: 0 162 951 993
Size: 162 789 42
Fragments arranged by size:
789 162 42
PstI C_TGCA'G
Cuts at: 0 876 993
Size: 876 117
SacI G_AGCT'C
Cuts at: 0 961 993
Size: 961 32
SapI GCTCTTCn'nnn_
Cuts at: 0 880 993
Size: 880 113
ScaI AGT'ACT
Cuts at: 0 311 993
Size: 311 682
SfcI C'TryA_G
Cuts at: 0 410 872 993
Size: 410 462 121
Fragments arranged by size:
462 410 121
SmaI CCC'GGG
Cuts at: 0 56 899 993
Size: 56 843 94
Fragments arranged by size:
843 94 56
SmlI C'TyrA_G
Cuts at: 0 265 891 993
Size: 265 626 102
Fragments arranged by size:
626 265 102
SphI G_CATG'C
Cuts at: 0 684 993
Size: 684 309
StuI AGG'CCT
Cuts at: 0 565 587 993
Size: 565 22 406
Fragments arranged by size:
565 406 22
StyI C'CwwG_G
Cuts at: 0 167 374 504 965 993
Size: 167 207 130 461 28
Fragments arranged by size:
461 207 167 130 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 772 993
Size: 772 221
TatI w'GTAC_w
Cuts at: 0 309 356 533 993
Size: 309 47 177 460
Fragments arranged by size:
460 309 177 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 993
Size: 299 32 662
Fragments arranged by size:
662 299 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 268 477 993
Size: 268 209 516
Fragments arranged by size:
516 268 209
XmaI C'CCGG_G
Cuts at: 0 54 897 993
Size: 54 843 96
Fragments arranged by size:
843 96 54
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApoI AvaI BaeI
BanII BbsI BciVI BfrBI BglI Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrGI BstAPI BstXI Bsu36I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I MmeI MslI
NcoI NsiI NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI SacI SapI ScaI SfcI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbvCI BcgI BclI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsiEI BsiWI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI