################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BbrPI BbsI BbvCI BciVI BfrI BpuAI BsaBI BseAI BsiHKAI BsiWI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HincII HindII HpaI KpnI MamI MfeI MluI MroI MunI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 1217 ACGTGT A^C[GA][CT]GT Aor51HI 1856 AGCGCT AGC^GCT AvrII 381 CCTAGG C^CTAGG BamHI 899 GGATCC G^GATCC BanI 1790 GGCGCC G^G[CT][GA]CC BbeI 1790 GGCGCC GGCGC^C BclI 1427 TGATCA T^GATCA BglII 1577 AGATCT A^GATCT BlnI 381 CCTAGG C^CTAGG BsaAI 1618 TACGTG [CT]AC^GT[GA] BsaWI 1829 TCCGGT [AT]^CCGG[AT] BsiEI 1815 CGACCG CG[GA][CT]^CG BsmBI 1438 GAGACG CGTCTC(1/5) BsmI 1402 GCATTC GAATGC(1/-1) Bsp1407I 1301 TGTACA T^GTACA BsrFI 1162 GCCGGC [GA]^CCGG[CT] BsrGI 1301 TGTACA T^GTACA BssSI 1348 CACGAG CACGAG(-5/-1) BstAPI 1347 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 735 GGTGACC G^GT[ACGT]ACC BstPI 735 GGTGACC G^GT[ACGT]ACC BstXI 1362 CCATGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1162 GCCGGC [GA]^CCGG[CT] Eco47III 1856 AGCGCT AGC^GCT EcoO65I 735 GGTGACC G^GT[ACGT]ACC EcoRI 712 GAATTC G^AATTC FbaI 1427 TGATCA T^GATCA HgaI 2071 GCGTC GACGC(5/10) HindIII 1267 AAGCTT A^AGCTT KasI 1790 GGCGCC G^GCGCC KspI 287 CCGCGG CCGC^GG MseI 1211 TTAA T^TAA NaeI 1162 GCCGGC GCC^GGC NarI 1790 GGCGCC GG^CGCC NdeI 1702 CATATG CA^TATG NgoMIV 1162 GCCGGC G^CCGGC NspI 231 ACATGC [GA]CATG^[CT] SacII 287 CCGCGG CCGC^GG SfoI 1790 GGCGCC GGC^GCC SspBI 1301 TGTACA T^GTACA Tru9I 1211 TTAA T^TAA XbaI 1851 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 712 GAATTC [GA]^AATT[CT] 979 GAATTT AcyI 1790 GGCGCC G[GA]^CG[CT]C 2070 GGCGTC ApoI 712 GAATTC [GA]^AATT[CT] 979 GAATTT BalI 2153 TGGCCA TGG^CCA 2202 TGGCCA BcgI 1923 CGAGGGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1373 GCAAGCTCTTCG BglI 541 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2033 GCCTTGAAGGC BlpI 186 GCTCAGC GC^T[ACGT]AGC 397 GCTCAGC Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC 397 GCTCAGC BsaHI 1790 GGCGCC G[GA]^CG[CT]C 2070 GGCGTC BsaI 1477 GAGACC GGTCTC(1/5) 1543 GGTCTC BspMI 436 ACCTGC ACCTGC(4/8) 1335 GCAGGT BsrBI 1030 GAGCGG CCGCTC(-3/-3) 1470 GAGCGG CelII 186 GCTCAGC GC^T[ACGT]AGC 397 GCTCAGC Hin1I 1790 GGCGCC G[GA]^CG[CT]C 2070 GGCGTC MluNI 2153 TGGCCA TGG^CCA 2202 TGGCCA MscI 2153 TGGCCA TGG^CCA 2202 TGGCCA NcoI 530 CCATGG C^CATGG 1362 CCATGG PflMI 765 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2113 CCAGAGGATGG PpuMI 209 AGGACCC [GA]G^G[AT]CC[CT] 1295 AGGACCT SmaI 740 CCCGGG CCC^GGG 1802 CCCGGG Van91I 765 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2113 CCAGAGGATGG XcmI 356 CCAAGGAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1889 CCACAGGGGATGTGG XmaCI 740 CCCGGG C^CCGGG 1802 CCCGGG XmaI 740 CCCGGG C^CCGGG 1802 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII