Restriction map for
pFN21AE4571
(Circular) (Six-base) MAPSORT of: pFN21AE4571.seq Check: 5757 from: 1 to: 6916
pFN21AE4571 6916 bp
With 182 enzymes: SgfI *
March 28, 2012 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3331 3331
Size: 6916
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4726 278
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4259 1937
Size: 2322 4594
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 5043 5416 1563
Size: 3480 373 3063
Fragments arranged by size:
3480 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2268 2726 3086 3863 3908 4979 6363
Size: 506 130 458 360 777 45 1071 1384
Cuts at: 6363 6631 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1071 777 506 458 360 268
130 45
AfeI AGC'GCT
Cuts at: 3863 3863
Size: 6916
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6719
AflIII A'CryG_T
Cuts at: 1203 3222 5815 6792 1203
Size: 2019 2593 977 1327
Fragments arranged by size:
2593 2019 1327 977
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 5645 5645
Size: 6916
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2253 1184
Size: 1069 5847
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2367 2399 2441 2473 3482 3514 2367
Size: 32 42 32 1009 32 5769
Fragments arranged by size:
5769 1009 42 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2836 3064 3886 4063 4085 4659 6231 2836
Size: 228 822 177 22 574 1572 3521
Fragments arranged by size:
3521 1572 822 574 228 177 22
ApaI G_GGCC'C
Cuts at: 3327 3922 4190 3327
Size: 595 268 6053
Fragments arranged by size:
6053 595 268
ApaLI G'TGCA_C
Cuts at: 4973 6129 4973
Size: 1156 5760
ApoI r'AATT_y
Cuts at: 1493 2717 2984 4225 4451 4603 6712 1493
Size: 1224 267 1241 226 152 2109 1697
Fragments arranged by size:
2109 1697 1241 1224 267 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5470 160
Size: 872 4438 1606
Fragments arranged by size:
4438 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 2745 2976 3807 3959 4130 4241 1870
Size: 73 802 231 831 152 171 111 4545
Fragments arranged by size:
4545 831 802 231 171 152 111 73
AvrII C'CTAG_G
Cuts at: 2386 2386
Size: 6916
BamHI G'GATC_C
Cuts at: 2904 4246 4613 2904
Size: 1342 367 5207
Fragments arranged by size:
5207 1342 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3795 4237 5692 618
Size: 325 217 627 2008 442 1455 1842
Fragments arranged by size:
2008 1842 1455 627 442 325 217
BanII G_rGCy'C
Cuts at: 1391 2870 2894 3327 3922 4190 4235 1391
Size: 1479 24 433 595 268 45 4072
Fragments arranged by size:
4072 1479 595 433 268 45 24
BbeI G_GCGC'C
Cuts at: 3799 3799
Size: 6916
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3367 3401 3917 3951 5107 5141 1903
Size: 34 1430 34 516 34 1156 34 3678
Fragments arranged by size:
3678 1430 1156 516 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4808 6018 4808
Size: 1210 5706
BclI T'GATC_A
Cuts at: 1548 3432 1548
Size: 1884 5032
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2552 4044 5527
Size: 107 122 71 631 605 880 1492 1483
Cuts at: 5527 136
Size: 1525
Fragments arranged by size:
1525 1492 1483 880 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3582 6911 3582
Size: 3329 3587
BlpI GC'TnA_GC
Cuts at: 2192 2403 4333 2192
Size: 211 1930 4775
Fragments arranged by size:
4775 1930 211
Bme1580I G_kGCm'C
Cuts at: 1790 3327 3922 4190 4977 6133 1790
Size: 1537 595 268 787 1156 2573
Fragments arranged by size:
2573 1537 1156 787 595 268
BmgBI CAC'GTC
Cuts at: 1318 3616 4054 1318
Size: 2298 438 4180
Fragments arranged by size:
4180 2298 438
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2011 2501 2836
Size: 416 53 83 399 24 594 490 335
Cuts at: 2836 4185 4647 5605 442
Size: 1349 462 958 1753
Fragments arranged by size:
1753 1349 958 594 490 462 416 399
335 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 2753 2810 3305 3499 3599 3686 3764
Size: 1247 267 57 495 194 100 87 78
Cuts at: 3764 5576 1239
Size: 1812 2579
Fragments arranged by size:
2579 1812 1247 495 267 194 100 87
78 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5041 5906 6204 5041
Size: 865 298 5753
Fragments arranged by size:
5753 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3476 3554 5579 882
Size: 617 1977 78 2025 2219
Fragments arranged by size:
2219 2025 1977 617 78
BsaAI yAC'GTr
Cuts at: 493 1204 3625 4707 493
Size: 711 2421 1082 2702
Fragments arranged by size:
2702 2421 1082 711
BsaBI GATnn'nnATC
Cuts at: 6910 6910
Size: 6916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4076 4723 5105
Size: 53 83 186 936 2263 280 647 382
Cuts at: 5105 5740 275
Size: 635 1451
Fragments arranged by size:
2263 1451 936 647 635 382 280 186
83 53
BsaWI w'CCGG_w
Cuts at: 3834 5349 5747 6021 6168 6579 3834
Size: 1515 398 274 147 411 4171
Fragments arranged by size:
4171 1515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 3110 3140 3757
Size: 30 165 30 1038 30 639 30 617
Cuts at: 3757 3787 1178
Size: 30 4307
Fragments arranged by size:
4307 1038 639 617 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2279 2994 3302 4154 1186
Size: 1090 3 715 308 852 3948
Fragments arranged by size:
3948 1090 852 715 308 3
BseYI C'CCAG_C
Cuts at: 3229 3361 6119 6615 3229
Size: 132 2758 496 3530
Fragments arranged by size:
3530 2758 496 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2213 3335 3359 4076 1163
Size: 573 477 1122 24 717 4003
Fragments arranged by size:
4003 1122 717 573 477 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3823 4366 5127 6155 1540
Size: 275 186 1822 543 761 1028 2301
Fragments arranged by size:
2301 1822 1028 761 543 275 186
BsiHKAI G_wGCw'C
Cuts at: 4977 5062 6133 4977
Size: 85 1071 5760
Fragments arranged by size:
5760 1071 85
BsmI GAATG_Cn'
Cuts at: 3407 4427 4520 3407
Size: 1020 93 5803
Fragments arranged by size:
5803 1020 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3437 3437
Size: 6916
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2870 2894 3327 3922 4190 4235 4977
Size: 399 1080 24 433 595 268 45 742
Cuts at: 4977 5062 6133 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1080 1071 742 595 433 399 268
85 45 24
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 4805 6535 4805
Size: 1730 5186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2450 3331 4271 844
Size: 1606 881 940 3489
Fragments arranged by size:
3489 1606 940 881
BsrBI CCG'CTC
Cuts at: 1394 1823 3037 3477 4803 1394
Size: 429 1214 440 1326 3507
Fragments arranged by size:
3507 1326 1214 440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5411 5585 65
Size: 1183 4163 174 1396
Fragments arranged by size:
4163 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3167 5560 5747 1700
Size: 110 1357 2393 187 2869
Fragments arranged by size:
2869 2393 1357 187 110
BsrGI T'GTAC_A
Cuts at: 96 3306 96
Size: 3210 3706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6916
BssSI C'ACGA_G
Cuts at: 3353 4976 5988 3353
Size: 1623 1012 4281
Fragments arranged by size:
4281 1623 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3358 794
Size: 2564 4352
BstBI TT'CG_AA
Cuts at: 5720 5720
Size: 6916
BstEII G'GTnAC_C
Cuts at: 1184 2740 1184
Size: 1556 5360
BstXI CCAn_nnnn'nTGG
Cuts at: 3374 3374
Size: 6916
BstYI r'GATC_y
Cuts at: 1972 2904 3582 3831 4143 4246 4613 4998 5015
Size: 932 678 249 312 103 367 385 17
Cuts at: 5015 6467 6553 6565 6911 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 932 678 385 367 346 312
249 103 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2166 3681 3977 2166
Size: 1515 296 5105
Fragments arranged by size:
5105 1515 296
BtgI C'CryG_G
Cuts at: 513 1065 2004 2292 2535 3367 513
Size: 552 939 288 243 832 4062
Fragments arranged by size:
4062 939 832 552 288 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3493 487
Size: 683 56 2267 3910
Fragments arranged by size:
3910 2267 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4432 5226 5246 3379
Size: 1053 794 20 5049
Fragments arranged by size:
5049 1053 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6916
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6881
DraI TTT'AAA
Cuts at: 4220 4220
Size: 6916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5923 809
Size: 507 4607 1802
Fragments arranged by size:
4607 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3906 4158 4207
Size: 54 1425 50 275 18 2076 252 49
Cuts at: 4207 4363 5252 6881 8
Size: 156 889 1629 43
Fragments arranged by size:
2076 1629 1425 889 275 252 156 54
50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4363 1537
Size: 275 2551 4090
Fragments arranged by size:
4090 2551 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2609 2702 3389 3519 4846 1353
Size: 854 402 93 687 130 1327 3423
Fragments arranged by size:
3423 1327 854 687 402 130 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2063 3803 5503 5877 6023
Size: 1064 138 435 39 1740 1700 374 146
Cuts at: 6023 387
Size: 1280
Fragments arranged by size:
1740 1700 1280 1064 435 374 146 138
39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2268 2486 2726 2753 2810 3086
Size: 393 506 130 218 240 27 57 276
Cuts at: 3086 3305 3499 3599 3686 3764 3863 3908 4979
Size: 219 194 100 87 78 99 45 1071
Cuts at: 4979 5576 6363 6631 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1071 787 597 506 393 276 268
240 219 218 194 130 100 99 87
78 57 45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2258 2771 3317 3926 2258
Size: 513 546 609 5248
Fragments arranged by size:
5248 609 546 513
EcoO109I rG'GnC_Cy
Cuts at: 2170 2215 2428 2918 3301 3323 3324 3919 4186
Size: 45 213 490 383 22 1 595 267
Cuts at: 4186 4187 4198 2170
Size: 1 11 4888
Fragments arranged by size:
4888 595 490 383 267 213 45 22
11 1 1
EcoRI G'AATT_C
Cuts at: 2717 4225 4603 2717
Size: 1508 378 5030
Fragments arranged by size:
5030 1508 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 6916
FspI TGC'GCA
Cuts at: 5422 5422
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 2098 3799 3865 6063 1678
Size: 420 1701 66 2198 2531
Fragments arranged by size:
2531 2198 1701 420 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3110 3142 3454 3486 3532
Size: 32 135 32 1538 32 312 32 46
Cuts at: 3532 3564 4187 4219 5557 5589 5631 5663 1373
Size: 32 623 32 1338 32 42 32 2626
Fragments arranged by size:
2626 1538 1338 623 312 135 46 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4260 4506 669
Size: 988 281 2322 246 3079
Fragments arranged by size:
3079 2322 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3272 748
Size: 2524 4392
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KasI G'GCGC_C
Cuts at: 3795 3795
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4515 4515
Size: 6916
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 4116 6030 6214 1305
Size: 1026 1785 1914 184 2007
Fragments arranged by size:
2007 1914 1785 1026 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4160 4209 10
Size: 54 1425 343 2328 49 2717
Fragments arranged by size:
2717 2328 1425 343 54 49
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2235 3372 4874 5233 5392 518
Size: 648 876 193 1137 1502 359 159 2042
Fragments arranged by size:
2042 1502 1137 876 648 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 2227 2294 2788 3061 3265 4082 4156 4332
Size: 621 67 494 273 204 817 74 176
Cuts at: 4332 5009 6157 6402 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 817 677 621 494 273 245
204 176 74 67
NaeI GCC'GGC
Cuts at: 3169 3169
Size: 6916
NarI GG'CG_CC
Cuts at: 3796 3796
Size: 6916
NcoI C'CATG_G
Cuts at: 513 1065 2004 2535 3367 513
Size: 552 939 531 832 4062
Fragments arranged by size:
4062 939 832 552 531
NdeI CA'TA_TG
Cuts at: 387 3708 387
Size: 3321 3595
NgoMIV G'CCGG_C
Cuts at: 3167 3167
Size: 6916
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 2240 4274 5819 1132
Size: 97 1011 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 1011 97
PasI CC'CwG_GG
Cuts at: 1348 2863 1348
Size: 1515 5401
PciI A'CATG_T
Cuts at: 5815 5815
Size: 6916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 4124 1108
Size: 1668 1348 3900
Fragments arranged by size:
3900 1668 1348
PfoI T'CCnGG_A
Cuts at: 2907 2907
Size: 6916
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2441 2473 4985 5017 6498 6530 2441
Size: 32 2512 32 1481 32 2827
Fragments arranged by size:
2827 2512 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2215 3301 2215
Size: 1086 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 4486 4486
Size: 6916
PspOMI G'GGCC_C
Cuts at: 3323 3918 4186 3323
Size: 595 268 6053
Fragments arranged by size:
6053 595 268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2261 2293 2261
Size: 32 6884
PstI C_TGCA'G
Cuts at: 830 4268 830
Size: 3438 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 2227 2788 3061 4082 4156 2227
Size: 561 273 1021 74 4987
Fragments arranged by size:
4987 1021 561 273 74
SacII CC_GC'GG
Cuts at: 2295 2295
Size: 6916
SalI G'TCGA_C
Cuts at: 1936 4258 1936
Size: 2322 4594
SapI GCTCTTCn'nnn_
Cuts at: 2609 2702 3389 2609
Size: 93 687 6136
Fragments arranged by size:
6136 687 93
SbfI CC_TGCA'GG
Cuts at: 4268 4268
Size: 6916
ScaI AGT'ACT
Cuts at: 5164 6876 5164
Size: 1712 5204
SfcI C'TryA_G
Cuts at: 826 1045 4264 5399 6080 6271 826
Size: 219 3219 1135 681 191 1471
Fragments arranged by size:
3219 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3797 3797
Size: 6916
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 2747 3809 4243 1872
Size: 875 1062 434 4545
Fragments arranged by size:
4545 1062 875 434
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5020 5921 6183 820
Size: 197 926 3077 901 262 1553
Fragments arranged by size:
3077 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4707 493
Size: 4214 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 4274 1132
Size: 3142 3774
SspI AAT'ATT
Cuts at: 5 52 4840 5
Size: 47 4788 2081
Fragments arranged by size:
4788 2081 47
StuI AGG'CCT
Cuts at: 1517 2069 2139 2253 2804 3686 1517
Size: 552 70 114 551 882 4747
Fragments arranged by size:
4747 882 552 551 114 70
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2386 2471 2535 2799 3367
Size: 552 939 357 25 85 64 264 568
Cuts at: 3367 4355 513
Size: 988 3074
Fragments arranged by size:
3074 988 939 568 552 357 264 85
64 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 2101 2427 2876 4943 4960 5113
Size: 1255 450 71 326 449 2067 17 153
Cuts at: 5113 5298 5743 325
Size: 185 445 1498
Fragments arranged by size:
2067 1498 1255 450 449 445 326 185
153 71 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 3306 5162
Size: 274 80 33 51 1443 42 1287 1856
Cuts at: 5162 6874 96
Size: 1712 138
Fragments arranged by size:
1856 1712 1443 1287 274 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6876 6908 6876
Size: 32 6884
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6916
XbaI T'CTAG_A
Cuts at: 3856 4252 3856
Size: 396 6520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 3901 2368
Size: 1533 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6916
XmaI C'CCGG_G
Cuts at: 1870 2745 3807 4241 1870
Size: 875 1062 434 4545
Fragments arranged by size:
4545 1062 875 434
XmnI GAAnn'nnTTC
Cuts at: 5045 5045
Size: 6916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4724 276
Size: 53 83 186 936 3190 2468
Fragments arranged by size:
3190 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI RsrII SacI SanDI SexAI SfiI SrfI
SwaI