(Linear) (Six-base) MAPSORT of: KIEE1456.seq Check: 7500 from: 1 to: 510 KIEE1456.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AatII G_ACGT'C Cuts at: 0 182 510 Size: 182 328 Acc65I G'GTAC_C Cuts at: 0 120 510 Size: 120 390 AflIII A'CryG_T Cuts at: 0 212 510 Size: 212 298 AhdI GACnn_n'nnGTC Cuts at: 0 183 510 Size: 183 327 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 199 231 346 378 510 Size: 199 32 115 32 132 Fragments arranged by size: 199 132 115 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 39 152 510 Size: 39 113 358 Fragments arranged by size: 358 113 39 BanI G'GyrC_C Cuts at: 0 120 510 Size: 120 390 BglI GCCn_nnn'nGGC Cuts at: 0 49 510 Size: 49 461 Bme1580I G_kGCm'C Cuts at: 0 68 510 Size: 68 442 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 510 Size: 184 326 BsaI GGTCTCn'nnnn_ Cuts at: 0 420 459 510 Size: 420 39 51 Fragments arranged by size: 420 51 39 BsaAI yAC'GTr Cuts at: 0 351 510 Size: 351 159 BsaBI GATnn'nnATC Cuts at: 0 269 510 Size: 269 241 BsaHI Gr'CG_yC Cuts at: 0 179 510 Size: 179 331 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 199 229 328 346 358 376 510 Size: 199 30 99 18 12 18 134 Fragments arranged by size: 199 134 99 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 438 462 465 510 Size: 438 24 3 45 Fragments arranged by size: 438 45 24 3 BseYI C'CCAG_C Cuts at: 0 71 91 196 510 Size: 71 20 105 314 Fragments arranged by size: 314 105 71 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 231 259 510 Size: 231 28 251 Fragments arranged by size: 251 231 28 BsiHKAI G_wGCw'C Cuts at: 0 212 510 Size: 212 298 BsmI GAATG_Cn' Cuts at: 0 391 510 Size: 391 119 BsmBI CGTCTCn'nnnn_ Cuts at: 0 286 510 Size: 286 224 Bsp1286I G_dGCh'C Cuts at: 0 68 212 510 Size: 68 144 298 Fragments arranged by size: 298 144 68 BspHI T'CATG_A Cuts at: 0 8 510 Size: 8 502 BsrFI r'CCGG_y Cuts at: 0 3 250 285 338 389 510 Size: 3 247 35 53 51 121 Fragments arranged by size: 247 121 53 51 35 3 BstAPI GCAn_nnn'nTGC Cuts at: 0 152 254 282 510 Size: 152 102 28 228 Fragments arranged by size: 228 152 102 28 BstXI CCAn_nnnn'nTGG Cuts at: 0 204 510 Size: 204 306 BstYI r'GATC_y Cuts at: 0 323 510 Size: 323 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 278 506 510 Size: 278 228 4 Fragments arranged by size: 278 228 4 BtsI GCAGTG_nn' Cuts at: 0 82 510 Size: 82 428 DraIII CAC_nnn'GTG Cuts at: 0 282 510 Size: 282 228 EaeI y'GGCC_r Cuts at: 0 41 296 510 Size: 41 255 214 Fragments arranged by size: 255 214 41 EarI CTCTTCn'nnn_ Cuts at: 0 439 510 Size: 439 71 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 510 Size: 184 326 HaeII r_GCGC'y Cuts at: 0 55 163 510 Size: 55 108 347 Fragments arranged by size: 347 108 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 169 201 328 351 360 383 510 Size: 169 32 127 23 9 23 127 Fragments arranged by size: 169 127 127 32 23 23 9 KpnI G_GTAC'C Cuts at: 0 124 510 Size: 124 386 NaeI GCC'GGC Cuts at: 0 391 510 Size: 391 119 NgoMIV G'CCGG_C Cuts at: 0 389 510 Size: 389 121 NspI r_CATG'y Cuts at: 0 216 510 Size: 216 294 PciI A'CATG_T Cuts at: 0 212 510 Size: 212 298 PfoI T'CCnGG_A Cuts at: 0 128 510 Size: 128 382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 199 231 346 378 510 Size: 199 32 115 32 132 Fragments arranged by size: 199 132 115 32 32 PstI C_TGCA'G Cuts at: 0 35 510 Size: 35 475 SfcI C'TryA_G Cuts at: 0 31 510 Size: 31 479 StyI C'CwwG_G Cuts at: 0 431 510 Size: 431 79 ZraI GAC'GTC Cuts at: 0 180 510 Size: 180 330 Enzymes that do cut: AatII Acc65I AflIII AhdI AloI AlwNI BanI BglI Bme1580I BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI BstAPI BstXI BstYI BtgZI BtsI DraIII EaeI EarI Eco57MI HaeII Hin4I KpnI NaeI NgoMIV NspI PciI PfoI PpiI PstI SfcI StyI ZraI Enzymes that do not cut: AarI AccI AclI AcuI AfeI AflII AgeI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaWI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI