################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaII AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MluNI MscI MseI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 28 GTCGAC GT^[AC][GT]AC AccIII 274 TCCGGA T^CCGGA AhdI 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 53 CCCGAG C^[CT]CG[GA]G BamHI 79 GGATCC G^GATCC BanI 199 GGTGCC G^G[CT][GA]CC BbsI 223 GAAGAC GAAGAC(2/6) BfaI 209 CTAG C^TAG BglII 288 AGATCT A^GATCT BmyI 385 GTGCCC G[AGT]GC[ACT]^C BpmI 68 CTCCAG CTGGAG(16/14) BpuAI 223 GAAGAC GAAGAC(2/6) BsaWI 274 TCCGGA [AT]^CCGG[AT] BseAI 274 TCCGGA T^CCGGA BseRI 258 CTCCTC GAGGAG(10/8) BsmAI 278 GAGAC GTCTC(1/5) BsmFI 157 GGGAC GGGAC(10/14) BsoBI 53 CCCGAG C^[CT]CG[GA]G Bsp1286I 385 GTGCCC G[AGT]GC[ACT]^C BspEI 274 TCCGGA T^CCGGA BspHI 312 TCATGA T^CATGA BspMI 196 GCAGGT ACCTGC(4/8) BsrI 409 CCAGT ACTGG(1/-1) BstF5I 416 CATCC GGATG(2/0) BstXI 389 CCAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 379 GCGC GCG^C DdeI 285 CTTAG C^T[ACGT]AG Eam1105I 226 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 358 GAAGAG CTCTTC(1/4) FokI 416 CATCC GGATG(9/13) HhaI 379 GCGC GCG^C HinP1I 379 GCGC G^CGC HincII 28 GTCGAC GT[CT]^[GA]AC HindII 28 GTCGAC GT[CT]^[GA]AC HindIII 176 AAGCTT A^AGCTT HphI 304 GGTGA GGTGA(8/7) Ksp632I 358 GAAGAG CTCTTC(1/4) MaeI 209 CTAG C^TAG MroI 274 TCCGGA T^CCGGA MslI 313 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 313 CATG CATG^ PflMI 70 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 244 CTGCAG CTGCA^G RcaI 312 TCATGA T^CATGA SalI 28 GTCGAC G^TCGAC SfcI 244 CTGCAG C^T[GA][CT]AG TaqI 29 TCGA T^CGA Tsp509I 85 AATT ^AATT TspRI 410 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 29 TCGA T^CGA Van91I 70 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 446 CCAGCCTCGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 63 GCGG CCGC(-3/-1) 437 GCGG AlwI 80 GATCC GGATC(4/5) 79 GGATC BbvI 341 GCAGC GCAGC(8/12) 376 GCTGC BcnI 393 CCCGG CC^[GC]GG 402 CCCGG BstYI 79 GGATCC [GA]^GATC[CT] 288 AGATCT Cac8I 268 GCAAGC GC[ACGT]^[ACGT]GC 372 GCCTGC DpnII 80 GATC ^GATC 289 GATC HinfI 26 GAGTC G^A[ACGT]TC 232 GAGTC MboI 80 GATC ^GATC 289 GATC MflI 79 GGATCC [GA]^GATC[CT] 288 AGATCT NciI 393 CCCGG CC^[GC]GG 402 CCCGG NdeII 80 GATC ^GATC 289 GATC PleI 26 GAGTC GAGTC(4/5) 232 GAGTC Sau3AI 80 GATC ^GATC 289 GATC TseI 341 GCAGC G^C[AT]GC 376 GCTGC XhoII 79 GGATCC [GA]^GATC[CT] 288 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI BsaJI BsiYI BslI BssKI BstNI Cfr13I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIV Sau96I ScrFI