(Linear) (Six-base) MAPSORT of: KIEE6124.seq  Check: 7192  from: 1  to: 462



KIEE6124.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     187     462 
   Size:        187     275
 
AccI GT'mk_AC

Cuts at:      0      29     462 
   Size:         29     433
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     284     313     462 
   Size:        185      99      29     149

  Fragments arranged by size: 

                 185     149      99      29
 
AhdI GACnn_n'nnGTC

Cuts at:      0     231     462 
   Size:        231     231
 
AvaI C'yCGr_G

Cuts at:      0      53     462 
   Size:         53     409
 
BamHI G'GATC_C

Cuts at:      0      79     462 
   Size:         79     383
 
BanI G'GyrC_C

Cuts at:      0     199     462 
   Size:        199     263
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     230     462 
   Size:        230     232
 
BglII A'GATC_T

Cuts at:      0     288     462 
   Size:        288     174
 
Bme1580I G_kGCm'C

Cuts at:      0     389     462 
   Size:        389      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     403     462 
   Size:        403      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     462 
   Size:         53     409
 
BsaWI w'CCGG_w

Cuts at:      0     274     462 
   Size:        274     188
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     249     462 
   Size:        249     213
 
BseYI C'CCAG_C

Cuts at:      0     388     462 
   Size:        388      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     389     462 
   Size:        389      73
 
BspEI T'CCGG_A

Cuts at:      0     274     462 
   Size:        274     188
 
BspHI T'CATG_A

Cuts at:      0     312     462 
   Size:        312     150
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     187     462 
   Size:        187     275
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     462 
   Size:        396      66
 
BstYI r'GATC_y

Cuts at:      0      79     288     462 
   Size:         79     209     174

  Fragments arranged by size: 

                 209     174      79
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     192     227     462 
   Size:        192      35     235

  Fragments arranged by size: 

                 235     192      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     353     462 
   Size:        353     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     185     284     313     462 
   Size:         53     132      99      29     149

  Fragments arranged by size: 

                 149     132      99      53      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249     462 
   Size:        217      32     213

  Fragments arranged by size: 

                 217     213      32
 
HincII GTy'rAC

Cuts at:      0      30     462 
   Size:         30     432
 
HindIII A'AGCT_T

Cuts at:      0     176     462 
   Size:        176     286
 
MslI CAynn'nnrTG

Cuts at:      0     317     462 
   Size:        317     145
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      76     462 
   Size:         76     386
 
PstI C_TGCA'G

Cuts at:      0     248     462 
   Size:        248     214
 
SalI G'TCGA_C

Cuts at:      0      28     462 
   Size:         28     434
 
SfcI C'TryA_G

Cuts at:      0     244     462 
   Size:        244     218
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     453     462 
   Size:        453       9
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AvaI    BamHI     BanI     BbsI
    BglII Bme1580I     BmrI     BpmI    BsaWI    BseRI    BseYI Bsp1286I
    BspEI    BspHI    BspMI    BstXI    BstYI    CspCI     EarI  Eco57MI
    Hin4I   HincII  HindIII     MslI    PflMI     PstI     SalI     SfcI
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI