(Circular) (Six-base) MAPSORT of: pFN21AE4439.seq Check: 8185 from: 1 to: 6073 pFN21AE4439 6073 bp With 182 enzymes: SgfI * March 28, 2012 14:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3416 1937 Size: 1479 4594 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 2980 4200 4573 1563 Size: 1417 1220 373 3063 Fragments arranged by size: 3063 1417 1220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4136 5520 5788 1632 Size: 2504 1384 268 1917 Fragments arranged by size: 2504 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 2136 4972 5949 1203 Size: 933 2836 977 1327 Fragments arranged by size: 2836 1327 977 933 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 2013 4802 2013 Size: 2789 3284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2424 1184 Size: 1240 4833 AlwNI CAG_nnn'CTG Cuts at: 2973 3081 3243 3816 5388 2973 Size: 108 162 573 1572 3658 Fragments arranged by size: 3658 1572 573 162 108 ApaI G_GGCC'C Cuts at: 3261 3261 Size: 6073 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2092 2693 3382 3608 3760 5869 1493 Size: 599 601 689 226 152 2109 1697 Fragments arranged by size: 2109 1697 689 601 599 226 152 AseI AT'TA_AT Cuts at: 160 1032 2454 4627 160 Size: 872 1422 2173 1606 Fragments arranged by size: 2173 1606 1422 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2732 3398 1870 Size: 73 789 666 4545 Fragments arranged by size: 4545 789 666 73 BamHI G'GATC_C Cuts at: 2319 2337 3403 3770 2319 Size: 18 1066 367 4622 Fragments arranged by size: 4622 1066 367 18 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 3394 4849 618 Size: 325 217 627 678 929 1455 1842 Fragments arranged by size: 1842 1455 929 678 627 325 217 BanII G_rGCy'C Cuts at: 1391 3261 3392 1391 Size: 1870 131 4072 Fragments arranged by size: 4072 1870 131 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3163 928 Size: 971 1264 3838 Fragments arranged by size: 3838 1264 971 BbvCI CC'TCA_GC Cuts at: 3279 3279 Size: 6073 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3164 3198 3235 3269 4264 4298 1903 Size: 34 1227 34 37 34 995 34 3678 Fragments arranged by size: 3678 1227 995 37 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 2376 2436 1548 Size: 828 60 5185 Fragments arranged by size: 5185 828 60 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2553 2570 3305 Size: 107 122 71 631 605 881 17 735 Cuts at: 3305 4684 136 Size: 1379 1525 Fragments arranged by size: 1525 1379 881 735 631 605 122 107 71 17 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3490 3490 Size: 6073 Bme1580I G_kGCm'C Cuts at: 1790 3261 4134 5290 1790 Size: 1471 873 1156 2573 Fragments arranged by size: 2573 1471 1156 873 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2320 3344 3804 Size: 416 53 83 399 24 903 1024 460 Cuts at: 3804 4762 442 Size: 958 1753 Fragments arranged by size: 1753 1024 958 903 460 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2823 4733 1239 Size: 1584 1910 2579 Fragments arranged by size: 2579 1910 1584 Bpu10I CC'TnA_GC Cuts at: 2217 3279 2217 Size: 1062 5011 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2702 4198 5063 5361 2702 Size: 1496 865 298 3414 Fragments arranged by size: 3414 1496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2738 3281 4736 882 Size: 617 1239 543 1455 2219 Fragments arranged by size: 2219 1455 1239 617 543 BsaAI yAC'GTr Cuts at: 493 1204 3864 493 Size: 711 2660 2702 Fragments arranged by size: 2702 2660 711 BsaBI GATnn'nnATC Cuts at: 2441 6067 2441 Size: 3626 2447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 2189 3880 4262 Size: 53 83 186 936 648 8 1691 382 Cuts at: 4262 4897 275 Size: 635 1451 Fragments arranged by size: 1691 1451 936 648 635 382 186 83 53 8 BsaWI w'CCGG_w Cuts at: 4506 4904 5178 5325 5736 4506 Size: 398 274 147 411 4843 Fragments arranged by size: 4843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 2735 2765 2796 Size: 30 165 30 882 30 420 30 31 Cuts at: 2796 2826 1178 Size: 30 4425 Fragments arranged by size: 4425 882 420 165 31 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 1186 Size: 1603 4470 BseYI C'CCAG_C Cuts at: 2065 5276 5772 2065 Size: 3211 496 2366 Fragments arranged by size: 3211 2366 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 1163 Size: 573 684 4816 Fragments arranged by size: 4816 684 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 3180 4134 4219 5290 2451 Size: 729 954 85 1071 3234 Fragments arranged by size: 3234 1071 954 729 85 BsiWI C'GTAC_G Cuts at: 2332 2332 Size: 6073 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 3180 3261 3392 4134 4219 5290 Size: 399 661 729 81 131 742 85 1071 Cuts at: 5290 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 729 661 399 131 85 81 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 2100 2253 2532 3962 5692 2100 Size: 153 279 1430 1730 2481 Fragments arranged by size: 2481 1730 1430 279 153 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2221 3960 1394 Size: 429 398 1739 3507 Fragments arranged by size: 3507 1739 429 398 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 6073 BstYI r'GATC_y Cuts at: 1972 2319 2337 3403 3770 4155 4172 5624 5710 Size: 347 18 1066 367 385 17 1452 86 Cuts at: 5710 5722 6068 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1066 385 367 347 346 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 2025 Size: 6073 BtgI C'CryG_G Cuts at: 513 1065 2087 2568 513 Size: 552 1022 481 4018 Fragments arranged by size: 4018 1022 552 481 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2941 487 Size: 683 56 1715 3619 Fragments arranged by size: 3619 1715 683 56 BtsI GCAGTG_nn' Cuts at: 2160 2379 3122 3589 4383 4403 2160 Size: 219 743 467 794 20 3830 Fragments arranged by size: 3830 794 743 467 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2355 2539 Size: 54 1425 50 275 18 276 249 184 Cuts at: 2539 2571 3520 4409 6038 8 Size: 32 949 889 1629 43 Fragments arranged by size: 1629 1425 949 889 276 275 249 184 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4003 1353 Size: 2650 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2823 4136 4733 5520 5788 1239 Size: 393 1191 1313 597 787 268 1524 Fragments arranged by size: 1524 1313 1191 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2393 2393 Size: 6073 EcoO109I rG'GnC_Cy Cuts at: 2545 2729 2545 Size: 184 5889 EcoRI G'AATT_C Cuts at: 2693 3382 3760 2693 Size: 689 378 5006 Fragments arranged by size: 5006 689 378 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2095 2127 2796 Size: 32 135 32 427 32 64 32 669 Cuts at: 2796 2828 2933 2965 4714 4746 4788 4820 1373 Size: 32 105 32 1749 32 42 32 2626 Fragments arranged by size: 2626 1749 669 427 135 105 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 2720 748 Size: 1972 4101 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3366 3672 3366 Size: 306 5767 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 2841 3246 5187 5371 1305 Size: 1428 108 405 1941 184 2007 Fragments arranged by size: 2007 1941 1428 405 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 2357 2541 2573 10 Size: 54 1425 343 525 184 32 3510 Fragments arranged by size: 3510 1425 525 343 184 54 32 MslI CAynn'nnrTG Cuts at: 518 1166 2390 2432 2918 2993 4031 4390 4549 Size: 648 1224 42 486 75 1038 359 159 Cuts at: 4549 518 Size: 2042 Fragments arranged by size: 2042 1224 1038 648 486 359 159 75 42 MspA1I CmG'CkG Cuts at: 1606 2404 3050 3081 3278 3489 4166 5314 5559 Size: 798 646 31 197 211 677 1148 245 Cuts at: 5559 1606 Size: 2120 Fragments arranged by size: 2120 1148 798 677 646 245 211 197 31 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5521 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 2140 3250 3431 4976 1132 Size: 97 911 1110 181 1545 2229 Fragments arranged by size: 2229 1545 1110 911 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 2136 4972 2136 Size: 2836 3237 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2729 2729 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 3167 1613 Size: 1554 4519 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PspOMI G'GGCC_C Cuts at: 3257 3257 Size: 6073 PstI C_TGCA'G Cuts at: 830 2206 2478 3425 830 Size: 1376 272 947 3478 Fragments arranged by size: 3478 1376 947 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2404 3050 3278 2404 Size: 646 228 5199 Fragments arranged by size: 5199 646 228 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 2478 3425 2478 Size: 947 5126 ScaI AGT'ACT Cuts at: 4321 6033 4321 Size: 1712 4361 SfcI C'TryA_G Cuts at: 826 1045 2202 2474 3421 4556 5237 5428 826 Size: 219 1157 272 947 1135 681 191 1471 Fragments arranged by size: 1471 1157 1135 947 681 272 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 2734 3400 1872 Size: 862 666 4545 Fragments arranged by size: 4545 862 666 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 4177 5078 5340 820 Size: 197 926 774 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 774 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2154 3512 513 Size: 552 1089 1358 3074 Fragments arranged by size: 3074 1358 1089 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4100 4117 4270 4455 4900 325 Size: 1255 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4319 6031 96 Size: 274 80 33 51 1443 2342 1712 138 Fragments arranged by size: 2342 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 2732 3398 1870 Size: 862 666 4545 Fragments arranged by size: 4545 862 666 XmnI GAAnn'nnTTC Cuts at: 2096 4202 2096 Size: 2106 3967 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI