################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BlnI BsaBI BsaI BseAI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MaeI MamI MfeI MroI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1566 GACGTC GACGT^C AccI 766 GTCTAC GT^[AC][GT]AC AcsI 287 AAATTC [GA]^AATT[CT] AflII 1867 CTTAAG C^TTAAG Alw44I 1665 GTGCAC G^TGCAC ApaI 694 GGGCCC GGGCC^C ApaLI 1665 GTGCAC G^TGCAC ApoI 287 AAATTC [GA]^AATT[CT] BanI 1287 GGTGCC G^G[CT][GA]CC BbsI 418 GTCTTC GAAGAC(2/6) BfrI 1867 CTTAAG C^TTAAG BglII 1151 AGATCT A^GATCT BlpI 1348 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1348 GCTGAGC GC^T[ACGT]AGC BpuAI 418 GTCTTC GAAGAC(2/6) BsaAI 892 TACGTG [CT]AC^GT[GA] BsaWI 458 TCCGGT [AT]^CCGG[AT] BsiWI 344 CGTACG C^GTACG BsmBI 882 CGTCTC CGTCTC(1/5) Bsp1407I 737 TGTACA T^GTACA BspHI 167 TCATGA T^CATGA BsrDI 842 GCAATG GCAATG(2/0) BsrGI 737 TGTACA T^GTACA BstAPI 1022 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 15 GGTCACC G^GT[ACGT]ACC BstPI 15 GGTCACC G^GT[ACGT]ACC CelII 1348 GCTGAGC GC^T[ACGT]AGC DrdI 124 GACTGCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 712 CTCTTC CTCTTC(1/4) EcoO65I 15 GGTCACC G^GT[ACGT]ACC EcoRV 1816 GATATC GAT^ATC HindIII 1693 AAGCTT A^AGCTT Ksp632I 712 CTCTTC CTCTTC(1/4) MluI 907 ACGCGT A^CGCGT MseI 1868 TTAA T^TAA NotI 1910 GCGGCCGC GC^GGCCGC PshAI 906 GACGCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 167 TCATGA T^CATGA SacI 226 GAGCTC GAGCT^C SmaI 935 CCCGGG CCC^GGG SmlI 1867 CTTAAG C^T[CT][GA]AG SphI 54 GCATGC GCATG^C SspBI 737 TGTACA T^GTACA Tru9I 1868 TTAA T^TAA Tsp509I 288 AATT ^AATT XcmI 1259 CCAGCCCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 935 CCCGGG C^CCGGG XmaI 935 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 65 ACGTGT A^C[GA][CT]GT 907 ACGCGT AlwNI 741 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG 970 CAGCCACTG AvaI 220 CCCGAG C^[CT]CG[GA]G 935 CCCGGG BpmI 652 CTGGAG CTGGAG(16/14) 1297 CTGGAG BsiEI 491 CGGCCG CG[GA][CT]^CG 1911 CGGCCG BsmAI 455 GTCTC GTCTC(1/5) 883 GTCTC BsoBI 220 CCCGAG C^[CT]CG[GA]G 935 CCCGGG BstXI 5 CCACCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1490 CCATGTGTGTGG EagI 491 CGGCCG C^GGCCG 1911 CGGCCG EclXI 491 CGGCCG C^GGCCG 1911 CGGCCG Eco52I 491 CGGCCG C^GGCCG 1911 CGGCCG HaeII 988 AGCGCC [GA]GCGC^[CT] 1070 AGCGCC NspI 54 GCATGC [GA]CATG^[CT] 1784 ACATGC SfiI 825 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1903 GGCCTGAGCGGCC StuI 1397 AGGCCT AGG^CCT 1902 AGGCCT TaqI 584 TCGA T^CGA 680 TCGA TthHB8I 584 TCGA T^CGA 680 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI XhoII