(Circular) (Six-base) MAPSORT of: pFN21AE9695.seq Check: 2428 from: 1 to: 6670 pFN21AE9695 6670 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3349 3957 3349 Size: 608 6062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3575 4480 278 Size: 53 83 186 936 2039 905 2468 Fragments arranged by size: 2468 2039 936 905 186 83 53 AccI GT'mk_AC Cuts at: 1937 2772 4013 1937 Size: 835 1241 4594 Fragments arranged by size: 4594 1241 835 Acc65I G'GTAC_C Cuts at: 3991 3991 Size: 6670 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 3770 4733 6117 6385 1632 Size: 1787 351 963 1384 268 1917 Fragments arranged by size: 1917 1787 1384 963 351 268 AflII C'TTAA_G Cuts at: 820 1017 3872 820 Size: 197 2855 3618 Fragments arranged by size: 3618 2855 197 AflIII A'CryG_T Cuts at: 1203 2070 2912 5569 6546 1203 Size: 867 842 2657 977 1327 Fragments arranged by size: 2657 1327 977 867 842 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 2015 1166 Size: 849 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3269 3301 3269 Size: 32 6638 AlwNI CAG_nnn'CTG Cuts at: 2751 2980 4413 5985 2751 Size: 229 1433 1572 3436 Fragments arranged by size: 3436 1572 1433 229 ApaI G_GGCC'C Cuts at: 2703 2703 Size: 6670 ApaLI G'TGCA_C Cuts at: 3670 4727 5883 3670 Size: 1057 1156 4457 Fragments arranged by size: 4457 1156 1057 ApoI r'AATT_y Cuts at: 1493 2292 3979 4205 4357 6466 1493 Size: 799 1687 226 152 2109 1697 Fragments arranged by size: 2109 1697 1687 799 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2940 3995 1870 Size: 73 282 715 1055 4545 Fragments arranged by size: 4545 1055 715 282 73 BamHI G'GATC_C Cuts at: 4000 4367 4000 Size: 367 6303 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 3991 5446 618 Size: 325 217 627 1505 699 1455 1842 Fragments arranged by size: 1842 1505 1455 699 627 325 217 BanII G_rGCy'C Cuts at: 1391 2235 2261 2703 3210 3390 3989 1391 Size: 844 26 442 507 180 599 4072 Fragments arranged by size: 4072 844 599 507 442 180 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2416 928 Size: 971 517 5182 Fragments arranged by size: 5182 971 517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2184 2837 3394 Size: 107 122 71 631 605 512 653 557 Cuts at: 3394 3915 3924 5281 136 Size: 521 9 1357 1525 Fragments arranged by size: 1525 1357 653 631 605 557 521 512 122 107 71 9 BglII A'GATC_T Cuts at: 3156 6665 3156 Size: 3509 3161 BlpI GC'TnA_GC Cuts at: 3354 4087 3354 Size: 733 5937 Bme1580I G_kGCm'C Cuts at: 1790 2442 2703 3674 4731 5887 1790 Size: 652 261 971 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 971 652 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2192 2827 3317 Size: 416 53 83 399 24 775 635 490 Cuts at: 3317 4401 5359 442 Size: 1084 958 1753 Fragments arranged by size: 1753 1084 958 775 635 490 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2678 3323 5330 1239 Size: 1439 645 2007 2579 Fragments arranged by size: 2579 2007 1439 645 Bpu10I CC'TnA_GC Cuts at: 2787 3911 2787 Size: 1124 5546 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4795 5660 5958 4795 Size: 865 298 5507 Fragments arranged by size: 5507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2899 4461 493 Size: 711 1695 1562 2702 Fragments arranged by size: 2702 1695 1562 711 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2492 3188 3572 4477 Size: 53 83 186 936 959 696 384 905 Cuts at: 4477 4859 5494 275 Size: 382 635 1451 Fragments arranged by size: 1451 959 936 905 696 635 384 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2463 5103 5501 5775 5922 6333 2463 Size: 2640 398 274 147 411 2800 Fragments arranged by size: 2800 2640 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2876 2906 1178 Size: 30 165 30 1473 30 4942 Fragments arranged by size: 4942 1473 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2881 3848 1186 Size: 1057 638 967 4008 Fragments arranged by size: 4008 1057 967 638 BseYI C'CCAG_C Cuts at: 2086 2778 2990 3023 3086 3263 3435 5873 6369 Size: 692 212 33 63 177 172 2438 496 Cuts at: 6369 2086 Size: 2387 Fragments arranged by size: 2438 2387 692 496 212 177 172 63 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2972 3201 3302 3327 3724 3929 1163 Size: 573 1236 229 101 25 397 205 3904 Fragments arranged by size: 3904 1236 573 397 229 205 101 25 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2499 3919 4120 4881 5909 1540 Size: 275 186 498 1420 201 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 498 275 201 186 BsiHKAI G_wGCw'C Cuts at: 2235 3510 3674 4731 4816 5887 2235 Size: 1275 164 1057 85 1071 3018 Fragments arranged by size: 3018 1275 1071 1057 164 85 BsiWI C'GTAC_G Cuts at: 2349 2349 Size: 6670 BsmI GAATG_Cn' Cuts at: 4181 4274 4181 Size: 93 6577 BsmBI CGTCTCn'nnnn_ Cuts at: 2893 2893 Size: 6670 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2261 2442 2703 3210 3390 3510 Size: 399 445 26 181 261 507 180 120 Cuts at: 3510 3674 3989 4731 4816 5887 1391 Size: 164 315 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 507 445 399 315 261 181 180 164 120 85 26 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2172 4559 6289 2172 Size: 2387 1730 2553 Fragments arranged by size: 2553 2387 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3041 3349 3957 4025 844 Size: 2197 308 608 68 3489 Fragments arranged by size: 3489 2197 608 308 68 BsrBI CCG'CTC Cuts at: 1394 1823 2791 3019 3915 4557 1394 Size: 429 968 228 896 642 3507 Fragments arranged by size: 3507 968 896 642 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 2854 5165 5339 65 Size: 1183 1606 2311 174 1396 Fragments arranged by size: 2311 1606 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 2537 2961 3852 5314 5501 1700 Size: 110 433 294 424 891 1462 187 2869 Fragments arranged by size: 2869 1462 891 433 424 294 187 110 BsrGI T'GTAC_A Cuts at: 96 2742 96 Size: 2646 4024 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 4730 5742 4730 Size: 1012 5658 BstAPI GCAn_nnn'nTGC Cuts at: 794 3033 794 Size: 2239 4431 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 2020 1184 Size: 836 5834 BstXI CCAn_nnnn'nTGG Cuts at: 2017 3502 2017 Size: 1485 5185 BstYI r'GATC_y Cuts at: 1972 2527 2905 3129 3156 3429 3474 4000 4367 Size: 555 378 224 27 273 45 526 367 Cuts at: 4367 4752 4769 6221 6307 6319 6665 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 555 526 385 378 367 346 273 224 86 45 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3273 3555 3922 513 Size: 552 939 1269 282 367 3261 Fragments arranged by size: 3261 1269 939 552 367 282 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5931 Fragments arranged by size: 5931 683 56 BtsI GCAGTG_nn' Cuts at: 2532 2762 2978 4186 4980 5000 2532 Size: 230 216 1208 794 20 4202 Fragments arranged by size: 4202 1208 794 230 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 2515 2768 3498 2515 Size: 253 730 5687 Fragments arranged by size: 5687 730 253 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 5677 809 Size: 507 819 3542 1802 Fragments arranged by size: 3542 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2241 2496 Size: 54 1425 50 275 18 177 234 255 Cuts at: 2496 2829 2838 3591 3916 3957 4117 5006 6635 Size: 333 9 753 325 41 160 889 1629 Cuts at: 6635 8 Size: 43 Fragments arranged by size: 1629 1425 889 753 333 325 275 255 234 177 160 54 50 43 41 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2496 3916 4117 1537 Size: 275 684 1420 201 4090 Fragments arranged by size: 4090 1420 684 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 4600 1353 Size: 1370 1877 3423 Fragments arranged by size: 3423 1877 1370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2201 5257 5631 5777 387 Size: 1064 138 612 3056 374 146 1280 Fragments arranged by size: 3056 1280 1064 612 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2233 Size: 6670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2678 3323 3419 3770 4733 5330 6117 Size: 393 1046 645 96 351 963 597 787 Cuts at: 6117 6385 1239 Size: 268 1524 Fragments arranged by size: 1524 1046 963 787 645 597 393 351 268 96 EcoO109I rG'GnC_Cy Cuts at: 2275 2573 2653 2944 3307 2275 Size: 298 80 291 363 5638 Fragments arranged by size: 5638 363 298 291 80 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 EcoRV GAT'ATC Cuts at: 3823 3823 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 2997 3079 5817 1678 Size: 1319 82 2738 2531 Fragments arranged by size: 2738 2531 1319 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3101 3133 3452 3484 5311 Size: 32 135 32 1529 32 319 32 1827 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1827 1529 319 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2140 2581 2815 3637 4014 4260 Size: 988 281 202 441 234 822 377 246 Cuts at: 4260 669 Size: 3079 Fragments arranged by size: 3079 988 822 441 377 281 246 234 202 HindIII A'AGCT_T Cuts at: 748 3698 748 Size: 2950 3720 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 3995 3995 Size: 6670 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 2912 6546 2912 Size: 3634 3036 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3164 3432 5784 5968 1305 Size: 1859 268 2352 184 2007 Fragments arranged by size: 2352 2007 1859 268 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2840 3593 3959 10 Size: 54 1425 343 177 831 753 366 2721 Fragments arranged by size: 2721 1425 831 753 366 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2306 3278 3500 3788 4628 4987 Size: 648 849 291 972 222 288 840 359 Cuts at: 4987 5146 518 Size: 159 2042 Fragments arranged by size: 2042 972 849 840 648 359 291 288 222 159 MspA1I CmG'CkG Cuts at: 1606 2748 3587 3766 3885 4086 4763 5911 6156 Size: 1142 839 179 119 201 677 1148 245 Cuts at: 6156 1606 Size: 2120 Fragments arranged by size: 2120 1148 1142 839 677 245 201 179 119 NaeI GCC'GGC Cuts at: 2245 2539 3854 2245 Size: 294 1315 5061 Fragments arranged by size: 5061 1315 294 NcoI C'CATG_G Cuts at: 513 1065 2004 3273 3555 3922 513 Size: 552 939 1269 282 367 3261 Fragments arranged by size: 3261 1269 939 552 367 282 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NgoMIV G'CCGG_C Cuts at: 2243 2537 3852 2243 Size: 294 1315 5061 Fragments arranged by size: 5061 1315 294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 3916 4117 3916 Size: 201 6469 NspI r_CATG'y Cuts at: 1132 1229 2063 3793 4028 5573 1132 Size: 97 834 1730 235 1545 2229 Fragments arranged by size: 2229 1730 1545 834 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6670 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2275 2653 2944 3307 2275 Size: 378 291 363 5638 Fragments arranged by size: 5638 378 363 291 PshAI GACnn'nnGTC Cuts at: 1613 2915 1613 Size: 1302 5368 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 2699 2699 Size: 6670 PstI C_TGCA'G Cuts at: 830 2333 3038 3708 3765 4022 830 Size: 1503 705 670 57 257 3478 Fragments arranged by size: 3478 1503 705 670 257 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 SacI G_AGCT'C Cuts at: 2235 2235 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SanDI GG'GwC_CC Cuts at: 2944 2944 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SfcI C'TryA_G Cuts at: 826 1045 2115 2329 3034 3704 3761 4018 5153 Size: 219 1070 214 705 670 57 257 1135 Cuts at: 5153 5834 6025 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1070 705 681 670 257 219 214 191 57 SfiI GGCCn_nnn'nGGCC Cuts at: 2837 3915 2837 Size: 1078 5592 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 2942 3997 1872 Size: 1070 1055 4545 Fragments arranged by size: 4545 1070 1055 SmlI C'TyrA_G Cuts at: 820 1017 1943 3872 4774 5675 5937 820 Size: 197 926 1929 902 901 262 1553 Fragments arranged by size: 1929 1553 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 2063 4028 1132 Size: 931 1965 3774 Fragments arranged by size: 3774 1965 931 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 3404 3909 1517 Size: 1887 505 4278 Fragments arranged by size: 4278 1887 505 StyI C'CwwG_G Cuts at: 513 1065 2004 3273 3348 3555 3922 4109 513 Size: 552 939 1269 75 207 367 187 3074 Fragments arranged by size: 3074 1269 939 552 367 207 187 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3361 3803 4697 4714 4867 5052 5497 Size: 1255 1781 442 894 17 153 185 445 Cuts at: 5497 325 Size: 1498 Fragments arranged by size: 1781 1498 1255 894 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 4916 6628 Size: 274 80 33 51 1443 765 2174 1712 Cuts at: 6628 96 Size: 138 Fragments arranged by size: 2174 1712 1443 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 6630 6662 2404 Size: 32 4194 32 2412 Fragments arranged by size: 4194 2412 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 4006 4006 Size: 6670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3271 3271 Size: 6670 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 2940 3995 1870 Size: 1070 1055 4545 Fragments arranged by size: 4545 1070 1055 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3573 4478 276 Size: 53 83 186 936 2039 905 2468 Fragments arranged by size: 2468 2039 936 905 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfoI SrfI SwaI