(Linear) (Six-base) MAPSORT of: KIEE9695.seq Check: 8310 from: 1 to: 1965 KIEE9695.orf With 182 enzymes: SgfI * December 5, 2014 13:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1344 1952 1965 Size: 1344 608 13 Fragments arranged by size: 1344 608 13 AatII G_ACGT'C Cuts at: 0 1570 1965 Size: 1570 395 AccI GT'mk_AC Cuts at: 0 767 1965 Size: 767 1198 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1414 1765 1965 Size: 1414 351 200 Fragments arranged by size: 1414 351 200 AflII C'TTAA_G Cuts at: 0 1867 1965 Size: 1867 98 AflIII A'CryG_T Cuts at: 0 65 907 1965 Size: 65 842 1058 Fragments arranged by size: 1058 842 65 AleI CACnn'nnGTG Cuts at: 0 10 1965 Size: 10 1955 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1264 1296 1965 Size: 1264 32 669 Fragments arranged by size: 1264 669 32 AlwNI CAG_nnn'CTG Cuts at: 0 746 975 1965 Size: 746 229 990 Fragments arranged by size: 990 746 229 ApaI G_GGCC'C Cuts at: 0 698 1965 Size: 698 1267 ApaLI G'TGCA_C Cuts at: 0 1665 1965 Size: 1665 300 ApoI r'AATT_y Cuts at: 0 287 1965 Size: 287 1678 AvaI C'yCGr_G Cuts at: 0 220 935 1965 Size: 220 715 1030 Fragments arranged by size: 1030 715 220 BanI G'GyrC_C Cuts at: 0 1287 1965 Size: 1287 678 BanII G_rGCy'C Cuts at: 0 230 256 698 1205 1385 1965 Size: 230 26 442 507 180 580 Fragments arranged by size: 580 507 442 230 180 26 BbsI GAAGACnn'nnnn_ Cuts at: 0 411 1965 Size: 411 1554 BglI GCCn_nnn'nGGC Cuts at: 0 179 832 1389 1910 1919 1965 Size: 179 653 557 521 9 46 Fragments arranged by size: 653 557 521 179 46 9 BglII A'GATC_T Cuts at: 0 1151 1965 Size: 1151 814 BlpI GC'TnA_GC Cuts at: 0 1349 1965 Size: 1349 616 Bme1580I G_kGCm'C Cuts at: 0 437 698 1669 1965 Size: 437 261 971 296 Fragments arranged by size: 971 437 296 261 BmrI ACTGGGnnnn_n' Cuts at: 0 187 822 1312 1965 Size: 187 635 490 653 Fragments arranged by size: 653 635 490 187 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1318 1965 Size: 673 645 647 Fragments arranged by size: 673 647 645 Bpu10I CC'TnA_GC Cuts at: 0 782 1906 1965 Size: 782 1124 59 Fragments arranged by size: 1124 782 59 BsaAI yAC'GTr Cuts at: 0 894 1965 Size: 894 1071 BsaHI Gr'CG_yC Cuts at: 0 487 1183 1567 1965 Size: 487 696 384 398 Fragments arranged by size: 696 487 398 384 BsaWI w'CCGG_w Cuts at: 0 458 1965 Size: 458 1507 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 871 901 1965 Size: 871 30 1064 Fragments arranged by size: 1064 871 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 238 876 1843 1965 Size: 238 638 967 122 Fragments arranged by size: 967 638 238 122 BseYI C'CCAG_C Cuts at: 0 81 773 985 1018 1081 1258 1430 1965 Size: 81 692 212 33 63 177 172 535 Fragments arranged by size: 692 535 212 177 172 81 63 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 967 1196 1297 1322 1719 1924 1965 Size: 967 229 101 25 397 205 41 Fragments arranged by size: 967 397 229 205 101 41 25 BsiEI CG_ry'CG Cuts at: 0 494 1914 1965 Size: 494 1420 51 Fragments arranged by size: 1420 494 51 BsiHKAI G_wGCw'C Cuts at: 0 230 1505 1669 1965 Size: 230 1275 164 296 Fragments arranged by size: 1275 296 230 164 BsiWI C'GTAC_G Cuts at: 0 344 1965 Size: 344 1621 BsmBI CGTCTCn'nnnn_ Cuts at: 0 888 1965 Size: 888 1077 Bsp1286I G_dGCh'C Cuts at: 0 230 256 437 698 1205 1385 1505 1669 Size: 230 26 181 261 507 180 120 164 Cuts at: 1669 1965 Size: 296 Fragments arranged by size: 507 296 261 230 181 180 164 120 26 BspHI T'CATG_A Cuts at: 0 167 1965 Size: 167 1798 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1036 1344 1952 1965 Size: 1036 308 608 13 Fragments arranged by size: 1036 608 308 13 BsrBI CCG'CTC Cuts at: 0 786 1014 1910 1965 Size: 786 228 896 55 Fragments arranged by size: 896 786 228 55 BsrDI GCAATG_nn' Cuts at: 0 849 1965 Size: 849 1116 BsrFI r'CCGG_y Cuts at: 0 238 532 956 1847 1965 Size: 238 294 424 891 118 Fragments arranged by size: 891 424 294 238 118 BsrGI T'GTAC_A Cuts at: 0 737 1965 Size: 737 1228 BstAPI GCAn_nnn'nTGC Cuts at: 0 1028 1965 Size: 1028 937 BstEII G'GTnAC_C Cuts at: 0 15 1965 Size: 15 1950 BstXI CCAn_nnnn'nTGG Cuts at: 0 12 1497 1965 Size: 12 1485 468 Fragments arranged by size: 1485 468 12 BstYI r'GATC_y Cuts at: 0 522 900 1124 1151 1424 1469 1965 Size: 522 378 224 27 273 45 496 Fragments arranged by size: 522 496 378 273 224 45 27 BtgI C'CryG_G Cuts at: 0 1268 1550 1917 1965 Size: 1268 282 367 48 Fragments arranged by size: 1268 367 282 48 BtsI GCAGTG_nn' Cuts at: 0 527 757 973 1965 Size: 527 230 216 992 Fragments arranged by size: 992 527 230 216 DraIII CAC_nnn'GTG Cuts at: 0 510 763 1493 1965 Size: 510 253 730 472 Fragments arranged by size: 730 510 472 253 DrdI GACnn_nn'nnGTC Cuts at: 0 130 1965 Size: 130 1835 EaeI y'GGCC_r Cuts at: 0 2 236 491 824 833 1586 1911 1952 Size: 2 234 255 333 9 753 325 41 Cuts at: 1952 1965 Size: 13 Fragments arranged by size: 753 333 325 255 234 41 13 9 2 EagI C'GGCC_G Cuts at: 0 491 1911 1965 Size: 491 1420 54 Fragments arranged by size: 1420 491 54 EarI CTCTTCn'nnn_ Cuts at: 0 718 1965 Size: 718 1247 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 196 1965 Size: 196 1769 EcoICRI GAG'CTC Cuts at: 0 228 1965 Size: 228 1737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1318 1414 1765 1965 Size: 673 645 96 351 200 Fragments arranged by size: 673 645 351 200 96 EcoO109I rG'GnC_Cy Cuts at: 0 270 568 648 939 1302 1965 Size: 270 298 80 291 363 663 Fragments arranged by size: 663 363 298 291 270 80 EcoRV GAT'ATC Cuts at: 0 1818 1965 Size: 1818 147 HaeII r_GCGC'y Cuts at: 0 992 1074 1965 Size: 992 82 891 Fragments arranged by size: 992 891 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1096 1128 1447 1479 1965 Size: 1096 32 319 32 486 Fragments arranged by size: 1096 486 319 32 32 HincII GTy'rAC Cuts at: 0 135 576 810 1632 1965 Size: 135 441 234 822 333 Fragments arranged by size: 822 441 333 234 135 HindIII A'AGCT_T Cuts at: 0 1693 1965 Size: 1693 272 MluI A'CGCG_T Cuts at: 0 907 1965 Size: 907 1058 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 1427 1965 Size: 1159 268 538 Fragments arranged by size: 1159 538 268 MscI TGG'CCA Cuts at: 0 4 835 1588 1954 1965 Size: 4 831 753 366 11 Fragments arranged by size: 831 753 366 11 4 MslI CAynn'nnrTG Cuts at: 0 10 301 1273 1495 1783 1965 Size: 10 291 972 222 288 182 Fragments arranged by size: 972 291 288 222 182 10 MspA1I CmG'CkG Cuts at: 0 743 1582 1761 1880 1965 Size: 743 839 179 119 85 Fragments arranged by size: 839 743 179 119 85 NaeI GCC'GGC Cuts at: 0 240 534 1849 1965 Size: 240 294 1315 116 Fragments arranged by size: 1315 294 240 116 NcoI C'CATG_G Cuts at: 0 1268 1550 1917 1965 Size: 1268 282 367 48 Fragments arranged by size: 1268 367 282 48 NgoMIV G'CCGG_C Cuts at: 0 238 532 1847 1965 Size: 238 294 1315 118 Fragments arranged by size: 1315 294 238 118 NotI GC'GGCC_GC Cuts at: 0 1911 1965 Size: 1911 54 NspI r_CATG'y Cuts at: 0 58 1788 1965 Size: 58 1730 177 Fragments arranged by size: 1730 177 58 PpuMI rG'GwC_Cy Cuts at: 0 270 648 939 1302 1965 Size: 270 378 291 363 663 Fragments arranged by size: 663 378 363 291 270 PshAI GACnn'nnGTC Cuts at: 0 910 1965 Size: 910 1055 PspOMI G'GGCC_C Cuts at: 0 694 1965 Size: 694 1271 PstI C_TGCA'G Cuts at: 0 328 1033 1703 1760 1965 Size: 328 705 670 57 205 Fragments arranged by size: 705 670 328 205 57 SacI G_AGCT'C Cuts at: 0 230 1965 Size: 230 1735 SanDI GG'GwC_CC Cuts at: 0 939 1965 Size: 939 1026 SfcI C'TryA_G Cuts at: 0 110 324 1029 1699 1756 1965 Size: 110 214 705 670 57 209 Fragments arranged by size: 705 670 214 209 110 57 SfiI GGCCn_nnn'nGGCC Cuts at: 0 832 1910 1965 Size: 832 1078 55 Fragments arranged by size: 1078 832 55 SmaI CCC'GGG Cuts at: 0 937 1965 Size: 937 1028 SmlI C'TyrA_G Cuts at: 0 1867 1965 Size: 1867 98 SphI G_CATG'C Cuts at: 0 58 1965 Size: 58 1907 StuI AGG'CCT Cuts at: 0 1399 1904 1965 Size: 1399 505 61 Fragments arranged by size: 1399 505 61 StyI C'CwwG_G Cuts at: 0 1268 1343 1550 1917 1965 Size: 1268 75 207 367 48 Fragments arranged by size: 1268 367 207 75 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1356 1798 1965 Size: 1356 442 167 Fragments arranged by size: 1356 442 167 TatI w'GTAC_w Cuts at: 0 737 1965 Size: 737 1228 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 399 431 1965 Size: 399 32 1534 Fragments arranged by size: 1534 399 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1266 1965 Size: 1266 699 XmaI C'CCGG_G Cuts at: 0 935 1965 Size: 935 1030 ZraI GAC'GTC Cuts at: 0 1568 1965 Size: 1568 397 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AleI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PpuMI PshAI PspOMI PstI SacI SanDI SfcI SfiI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaI BsaBI BsmI BspEI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI KpnI MfeI NarI NdeI NheI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI