################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AvrII BalI BamHI BanII BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstF5I BstXI BstYI BstZ17I Bsu36I CelII ClaI DpnII DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI HindIII HpaI Ksp632I MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I XbaI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 90 GACGTC GACGT^C AgeI 326 ACCGGT A^CCGGT Alw44I 193 GTGCAC G^TGCAC ApaLI 193 GTGCAC G^TGCAC Asp700I 345 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 398 TGCGCA TGC^GCA BbeI 30 GGCGCC GGCGC^C BbvCI 570 CCTCAGC CCTCAGC(-5/-2) BsaWI 326 ACCGGT [AT]^CCGG[AT] BseRI 25 GAGGAG GAGGAG(10/8) BsiEI 582 CGGTCG CG[GA][CT]^CG BsmFI 209 GGGAC GGGAC(10/14) BsrBI 154 GAGCGG CCGCTC(-3/-3) BsrFI 326 ACCGGT [GA]^CCGG[CT] BsrI 414 ACTGG ACTGG(1/-1) BstEII 624 GGTGACC G^GT[ACGT]ACC BstPI 624 GGTGACC G^GT[ACGT]ACC Cfr10I 326 ACCGGT [GA]^CCGG[CT] CpoI 46 CGGTCCG CG^G[AT]CCG DdeI 571 CTCAG C^T[ACGT]AG DraII 118 GGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 262 GACGCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 118 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 624 GGTGACC G^GT[ACGT]ACC FspI 398 TGCGCA TGC^GCA HaeII 30 GGCGCC [GA]GCGC^[CT] HapII 327 CCGG C^CGG HincII 166 GTCAAC GT[CT]^[GA]AC HindII 166 GTCAAC GT[CT]^[GA]AC HinfI 345 GAATC G^A[ACGT]TC HpaII 327 CCGG C^CGG KasI 30 GGCGCC G^GCGCC MseI 323 TTAA T^TAA MslI 372 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 327 CCGG C^CGG NarI 30 GGCGCC GG^CGCC PaeR7I 505 CTCGAG C^TCGAG PflFI 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 468 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 326 ACCGGT A^CCGGT RsrII 46 CGGTCCG CG^G[AT]CCG SfoI 30 GGCGCC GGC^GCC SmlI 505 CTCGAG C^T[CT][GA]AG TfiI 345 GAATC G^A[AT]TC Tru9I 323 TTAA T^TAA TspRI 413 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 529 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 468 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 368 CCATCATCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 505 CTCGAG C^TCGAG XmnI 345 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 332 GGTACC G^GTACC 363 GGTACC Asp718I 332 GGTACC G^GTACC 363 GGTACC AspHI 193 GTGCAC G[AT]GC[AT]^C 510 GTGCTC AvaI 127 CCCGAG C^[CT]CG[GA]G 505 CTCGAG BglI 112 GCCGATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 559 GCCGAGGTGGC BmyI 193 GTGCAC G[AGT]GC[ACT]^C 510 GTGCTC BsiHKAI 193 GTGCAC G[AT]GC[AT]^C 510 GTGCTC BsoBI 127 CCCGAG C^[CT]CG[GA]G 505 CTCGAG Bsp1286I 193 GTGCAC G[AGT]GC[ACT]^C 510 GTGCTC BspMI 170 ACCTGC ACCTGC(4/8) 395 ACCTGC EcoNI 396 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 455 CCTACCACAGG FauI 19 GCGGG CCCGC(4/6) 83 GCGGG HgaI 262 GACGC GACGC(5/10) 269 GCGTC KpnI 332 GGTACC GGTAC^C 363 GGTACC KspI 17 CCGCGG CCGC^GG 579 CCGCGG MaeII 91 ACGT A^CGT 533 ACGT MspA1I 17 CCGCGG C[AC]G^C[GT]G 579 CCGCGG NlaIII 162 CATG CATG^ 526 CATG SacII 17 CCGCGG CCGC^GG 579 CCGCGG TaqI 287 TCGA T^CGA 506 TCGA TthHB8I 287 TCGA T^CGA 506 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BanI BbsI BbvI BpuAI BsaHI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EcoRII Fnu4HI HaeIII HhaI Hin1I HinP1I HphI ItaI MaeIII MboII MnlI MvaI MvnI MwoI NlaIV RsaI Sau96I ScrFI TseI Tsp45I