################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 762 CTTAAG C^TTAAG AocI 968 CCTTAGG CC^T[ACGT]AGG BalI 740 TGGCCA TGG^CCA BamHI 597 GGATCC G^GATCC BanI 568 GGTGCC G^G[CT][GA]CC BfrI 762 CTTAAG C^TTAAG BlpI 392 GCTCAGC GC^T[ACGT]AGC Bpu1102I 392 GCTCAGC GC^T[ACGT]AGC BsgI 292 GTGCAG GTGCAG(16/14) BsiEI 647 CGATCG CG[GA][CT]^CG BsmBI 223 CGTCTC CGTCTC(1/5) BspDI 94 ATCGAT AT^CGAT BspHI 353 TCATGA T^CATGA BspMI 422 ACCTGC ACCTGC(4/8) BsrBI 171 CCGCTC CCGCTC(-3/-3) BsrI 417 CCAGT ACTGG(1/-1) BstXI 573 CCATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 968 CCTTAGG CC^T[ACGT]AGG CelII 392 GCTCAGC GC^T[ACGT]AGC ClaI 94 ATCGAT AT^CGAT EaeI 740 TGGCCA [CT]^GGCC[GA] EarI 226 CTCTTC CTCTTC(1/4) Eco81I 968 CCTTAGG CC^T[ACGT]AGG EcoT14I 743 CCAAGG C^C[AT][AT]GG HaeII 1001 GGCGCT [GA]GCGC^[CT] HgaI 222 GCGTC GACGC(5/10) HincII 319 GTTAAC GT[CT]^[GA]AC HindII 319 GTTAAC GT[CT]^[GA]AC HpaI 319 GTTAAC GTT^AAC Ksp632I 226 CTCTTC CTCTTC(1/4) MluNI 740 TGGCCA TGG^CCA MscI 740 TGGCCA TGG^CCA PleI 286 GAGTC GAGTC(4/5) PpuMI 506 GGGACCT [GA]G^G[AT]CC[CT] PstI 832 CTGCAG CTGCA^G PvuI 647 CGATCG CGAT^CG RcaI 353 TCATGA T^CATGA SexAI 377 ACCTGGT A^CC[AT]GGT StuI 857 AGGCCT AGG^CCT StyI 743 CCAAGG C^C[AT][AT]GG TfiI 1017 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BseRI 187 GAGGAG GAGGAG(10/8) 946 GAGGAG BsmAI 224 GTCTC GTCTC(1/5) 634 GTCTC BsmFI 331 GGGAC GGGAC(10/14) 506 GGGAC BsrDI 753 CATTGC GCAATG(2/0) 680 GCAATG CfoI 549 GCGC GCG^C 1002 GCGC DraII 506 GGGACCT [GA]G^G[ACGT]CC[CT] 662 AGGCCCT DraIII 49 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 364 CACGGGGTG EcoNI 429 CCTTGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 510 CCTGTGCCAGG EcoO109I 506 GGGACCT [GA]G^G[ACGT]CC[CT] 662 AGGCCCT HhaI 549 GCGC GCG^C 1002 GCGC HinP1I 549 GCGC G^CGC 1002 GCGC HinfI 286 GAGTC G^A[ACGT]TC 1017 GAATC MaeIII 19 GTAAC ^GT[ACGT]AC 920 GTTAC MseI 320 TTAA T^TAA 763 TTAA SfcI 217 CTACAG C^T[GA][CT]AG 832 CTGCAG SmlI 271 CTCAAG C^T[CT][GA]AG 762 CTTAAG TaqI 95 TCGA T^CGA 491 TCGA Tru9I 320 TTAA T^TAA 763 TTAA TthHB8I 95 TCGA T^CGA 491 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI TseI TspRI Van91I XcmI XhoII