(Circular) (Six-base) MAPSORT of: pFN21AE4337.seq Check: 1312 from: 1 to: 5749 pFN21AE4337 5749 bp With 182 enzymes: SgfI * March 28, 2012 13:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3012 3812 5196 5464 1632 Size: 1380 800 1384 268 1917 Fragments arranged by size: 1917 1384 1380 800 268 AflII C'TTAA_G Cuts at: 820 1017 2767 820 Size: 197 1750 3802 Fragments arranged by size: 3802 1750 197 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2167 2350 2405 2428 3492 5064 2167 Size: 183 55 23 1064 1572 2852 Fragments arranged by size: 2852 1572 1064 183 55 23 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 2602 3079 3446 2602 Size: 477 367 4905 Fragments arranged by size: 4905 477 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2573 3070 4525 618 Size: 325 217 627 786 497 1455 1842 Fragments arranged by size: 1842 1455 786 627 497 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2221 2277 3068 1391 Size: 744 86 56 791 4072 Fragments arranged by size: 4072 791 744 86 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 2398 3166 2398 Size: 768 4981 Bme1580I G_kGCm'C Cuts at: 1790 2578 2816 2822 3810 4966 1790 Size: 788 238 6 988 1156 2573 Fragments arranged by size: 2573 1156 988 788 238 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2416 3480 4438 Size: 416 53 83 399 24 999 1064 958 Cuts at: 4438 442 Size: 1753 Fragments arranged by size: 1753 1064 999 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2881 2881 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2093 2372 4409 1239 Size: 845 9 279 2037 2579 Fragments arranged by size: 2579 2037 845 279 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 3874 4739 5037 2261 Size: 1613 865 298 2973 Fragments arranged by size: 2973 1613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3540 493 Size: 711 2336 2702 Fragments arranged by size: 2702 2336 711 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2945 2975 1178 Size: 30 165 30 1542 30 3952 Fragments arranged by size: 3952 1542 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2966 1186 Size: 1021 759 3969 Fragments arranged by size: 3969 1021 759 BseYI C'CCAG_C Cuts at: 2936 2956 4952 5448 2936 Size: 20 1996 496 3237 Fragments arranged by size: 3237 1996 496 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 1163 Size: 573 582 4594 Fragments arranged by size: 4594 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2655 3199 3960 4988 1540 Size: 275 186 654 544 761 1028 2301 Fragments arranged by size: 2301 1028 761 654 544 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 BsmBI CGTCTCn'nnnn_ Cuts at: 2234 2234 Size: 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2221 2277 2578 2816 2822 3068 Size: 399 345 86 56 301 238 6 246 Cuts at: 3068 3810 3895 4966 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 345 301 246 238 86 85 56 6 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 2358 3638 5368 2358 Size: 1280 1730 2739 Fragments arranged by size: 2739 1730 1280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2436 3104 844 Size: 1592 668 3489 Fragments arranged by size: 3489 1592 668 BsrBI CCG'CTC Cuts at: 1394 1823 2178 3636 1394 Size: 429 355 1458 3507 Fragments arranged by size: 3507 1458 429 355 BsrDI GCAATG_nn' Cuts at: 65 1248 2692 2757 4244 4418 65 Size: 1183 1444 65 1487 174 1396 Fragments arranged by size: 1487 1444 1396 1183 174 65 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2585 2585 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2602 2740 2799 2877 2998 3079 3446 3831 Size: 630 138 59 78 121 81 367 385 Cuts at: 3831 3848 5300 5386 5398 5744 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 630 385 367 346 138 121 86 81 78 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2974 2974 Size: 5749 BtgI C'CryG_G Cuts at: 513 1065 2004 2122 2283 2368 2451 513 Size: 552 939 118 161 85 83 3811 Fragments arranged by size: 3811 939 552 161 118 85 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2550 3265 4059 4079 2550 Size: 715 794 20 4220 Fragments arranged by size: 4220 794 715 20 ClaI AT'CG_AT Cuts at: 1554 2100 1554 Size: 546 5203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DraIII CAC_nnn'GTG Cuts at: 2059 2374 2059 Size: 315 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2745 3196 4085 Size: 54 1425 50 275 18 915 451 889 Cuts at: 4085 5714 8 Size: 1629 43 Fragments arranged by size: 1629 1425 915 889 451 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 3679 1353 Size: 884 1442 3423 Fragments arranged by size: 3423 1442 884 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2093 2372 3012 3812 4409 5196 Size: 393 452 9 279 640 800 597 787 Cuts at: 5196 5464 1239 Size: 268 1524 Fragments arranged by size: 1524 800 787 640 597 452 393 279 268 9 EcoNI CCTnn'n_nnAGG Cuts at: 2438 2519 2438 Size: 81 5668 EcoO109I rG'GnC_Cy Cuts at: 2512 2668 2512 Size: 156 5593 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 5749 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 3010 4896 1678 Size: 1332 1886 2531 Fragments arranged by size: 2531 1886 1332 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373 Size: 32 135 32 2818 32 42 32 2626 Fragments arranged by size: 2818 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2326 3093 3339 669 Size: 988 281 388 767 246 3079 Fragments arranged by size: 3079 988 767 388 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 2326 3339 2326 Size: 1013 4736 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 10 Size: 54 1425 343 915 3012 Fragments arranged by size: 3012 1425 915 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3707 4066 4225 518 Size: 648 2541 359 159 2042 Fragments arranged by size: 2541 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3165 3842 4990 5235 1606 Size: 1559 677 1148 245 2120 Fragments arranged by size: 2120 1559 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4258 Fragments arranged by size: 4258 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 2608 1348 Size: 1260 4489 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2350 2737 2854 1108 Size: 1242 387 117 4003 Fragments arranged by size: 4003 1242 387 117 PfoI T'CCnGG_A Cuts at: 2160 2508 2718 2160 Size: 348 210 5191 Fragments arranged by size: 5191 348 210 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2512 Size: 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2408 2440 2408 Size: 32 5717 PstI C_TGCA'G Cuts at: 830 2841 3101 830 Size: 2011 260 3478 Fragments arranged by size: 3478 2011 260 PvuI CG_AT'CG Cuts at: 2001 2655 2001 Size: 654 5095 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SexAI A'CCwGG_T Cuts at: 2382 2382 Size: 5749 SfcI C'TryA_G Cuts at: 826 1045 2222 2837 3097 4232 4913 5104 826 Size: 219 1177 615 260 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 681 615 260 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2767 3853 4754 5016 820 Size: 197 926 333 491 1086 901 262 1553 Fragments arranged by size: 1553 1086 926 901 491 333 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2864 1517 Size: 1347 4402 StyI C'CwwG_G Cuts at: 513 1065 2004 2748 3188 513 Size: 552 939 744 440 3074 Fragments arranged by size: 3074 939 744 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 3995 5707 Size: 274 80 33 51 1443 264 1754 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1754 1712 1443 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2975 2977 3007 5709 5741 2945 Size: 30 2 30 2702 32 2953 Fragments arranged by size: 2953 2702 32 30 30 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2347 2453 2938 2347 Size: 106 485 5158 Fragments arranged by size: 5158 485 106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI