(Circular) (Six-base) MAPSORT of: pFN21AB5572.seq Check: 2424 from: 1 to: 5848 pFN21AB5572 5848 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2087 2087 Size: 5848 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 2645 3911 5295 5563 1632 Size: 653 360 1266 1384 268 1917 Fragments arranged by size: 1917 1384 1266 653 360 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 2369 4747 5724 1203 Size: 1166 2378 977 1327 Fragments arranged by size: 2378 1327 1166 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2438 2406 Size: 32 5816 AlwNI CAG_nnn'CTG Cuts at: 2301 3591 5163 2301 Size: 1290 1572 2986 Fragments arranged by size: 2986 1572 1290 ApaLI G'TGCA_C Cuts at: 2365 3905 5061 2365 Size: 1540 1156 3152 Fragments arranged by size: 3152 1540 1156 ApoI r'AATT_y Cuts at: 1493 3137 3157 3383 3535 5644 1493 Size: 1644 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 1644 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2246 2381 3169 4624 Size: 325 217 627 323 136 135 788 1455 Cuts at: 4624 618 Size: 1842 Fragments arranged by size: 1842 1455 788 627 325 323 217 136 135 BanII G_rGCy'C Cuts at: 1391 2012 2284 3167 1391 Size: 621 272 883 4072 Fragments arranged by size: 4072 883 621 272 BbeI G_GCGC'C Cuts at: 2114 2250 2385 2114 Size: 136 135 5577 Fragments arranged by size: 5577 136 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2541 4459 136 Size: 107 122 71 631 605 869 1918 1525 Fragments arranged by size: 1918 1525 869 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2369 3909 5065 1790 Size: 579 1540 1156 2573 Fragments arranged by size: 2573 1540 1156 579 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3579 4537 Size: 416 53 83 399 24 1293 869 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1293 958 869 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2299 4508 1239 Size: 887 173 2209 2579 Fragments arranged by size: 2579 2209 887 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2247 2382 3655 Size: 53 83 186 936 578 136 135 1273 Cuts at: 3655 4037 4672 275 Size: 382 635 1451 Fragments arranged by size: 1451 1273 936 635 578 382 186 136 135 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 1178 Size: 30 165 30 1003 30 4590 Fragments arranged by size: 4590 1003 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 1186 Size: 1309 4539 BseYI C'CCAG_C Cuts at: 2551 2979 5051 5547 2551 Size: 428 2072 496 2852 Fragments arranged by size: 2852 2072 496 428 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2956 1163 Size: 573 1220 4055 Fragments arranged by size: 4055 1220 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3298 4059 5087 1540 Size: 275 186 964 333 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 333 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2284 2369 2723 3909 3994 5065 2012 Size: 272 85 354 1186 85 1071 2795 Fragments arranged by size: 2795 1186 1071 354 272 85 85 BsmI GAATG_Cn' Cuts at: 2240 2394 3359 3452 2240 Size: 154 965 93 4636 Fragments arranged by size: 4636 965 154 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2284 2369 2723 3167 3909 3994 Size: 399 222 272 85 354 444 742 85 Cuts at: 3994 5065 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 444 399 354 272 222 85 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3045 3737 5467 3045 Size: 692 1730 3426 Fragments arranged by size: 3426 1730 692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2087 3203 844 Size: 1243 1116 3489 Fragments arranged by size: 3489 1243 1116 BsrBI CCG'CTC Cuts at: 1394 1823 2261 3735 1394 Size: 429 438 1474 3507 Fragments arranged by size: 3507 1474 438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 4492 4679 1700 Size: 110 1121 1561 187 2869 Fragments arranged by size: 2869 1561 1121 187 110 BsrGI T'GTAC_A Cuts at: 96 2832 96 Size: 2736 3112 BssHII G'CGCG_C Cuts at: 1925 2121 1925 Size: 196 5652 BssSI C'ACGA_G Cuts at: 2751 3908 4920 2751 Size: 1157 1012 3679 Fragments arranged by size: 3679 1157 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2859 794 Size: 2065 3783 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2230 2230 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2271 3178 3545 3930 3947 5399 5485 5497 Size: 299 907 367 385 17 1452 86 12 Cuts at: 5497 5843 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 385 367 346 299 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2385 2517 513 Size: 552 1320 132 3844 Fragments arranged by size: 3844 1320 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2002 487 Size: 683 56 776 4333 Fragments arranged by size: 4333 776 683 56 BtsI GCAGTG_nn' Cuts at: 2095 2477 3364 4158 4178 2095 Size: 382 887 794 20 3765 Fragments arranged by size: 3765 887 794 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3295 4184 Size: 54 1425 50 275 18 1132 333 889 Cuts at: 4184 5813 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1132 889 333 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 3295 1537 Size: 275 1150 333 4090 Fragments arranged by size: 4090 1150 333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3778 1353 Size: 2425 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2010 2282 2010 Size: 272 5576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2285 2299 2645 3911 4508 5295 Size: 393 494 159 14 346 1266 597 787 Cuts at: 5295 5563 1239 Size: 268 1524 Fragments arranged by size: 1524 1266 787 597 494 393 346 268 159 14 EcoNI CCTnn'n_nnAGG Cuts at: 2543 2676 2846 3112 2543 Size: 133 170 266 5279 Fragments arranged by size: 5279 266 170 133 EcoO109I rG'GnC_Cy Cuts at: 2512 2512 Size: 5848 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2030 2072 2114 2250 2385 4995 1678 Size: 352 42 42 136 135 2610 2531 Fragments arranged by size: 2610 2531 352 136 135 42 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2580 3192 3438 669 Size: 988 281 642 612 246 3079 Fragments arranged by size: 3079 988 642 612 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KasI G'GCGC_C Cuts at: 2110 2246 2381 2110 Size: 136 135 5577 Fragments arranged by size: 5577 136 135 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2587 4962 5146 1305 Size: 1282 2375 184 2007 Fragments arranged by size: 2375 2007 1282 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3090 3806 4165 4324 518 Size: 648 1924 716 359 159 2042 Fragments arranged by size: 2042 1924 716 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 2983 3012 3264 3941 5089 5334 1606 Size: 481 896 29 252 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 481 252 245 29 NarI GG'CG_CC Cuts at: 2111 2247 2382 2111 Size: 136 135 5577 Fragments arranged by size: 5577 136 135 NcoI C'CATG_G Cuts at: 513 1065 2517 513 Size: 552 1452 3844 Fragments arranged by size: 3844 1452 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2373 3089 3206 4751 1132 Size: 97 1144 716 117 1545 2229 Fragments arranged by size: 2229 1545 1144 716 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 2369 4747 2369 Size: 2378 3470 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PfoI T'CCnGG_A Cuts at: 2142 2142 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2406 2438 3917 3949 5430 5462 2406 Size: 32 1479 32 1481 32 2792 Fragments arranged by size: 2792 1481 1479 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2512 2512 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 2198 2279 3200 830 Size: 1368 81 921 3478 Fragments arranged by size: 3478 1368 921 81 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2983 2983 Size: 5848 SacI G_AGCT'C Cuts at: 2012 2284 2012 Size: 272 5576 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 2942 4096 5808 2942 Size: 1154 1712 2982 Fragments arranged by size: 2982 1712 1154 SfcI C'TryA_G Cuts at: 826 1045 2194 2275 3112 3196 4331 5012 5203 Size: 219 1149 81 837 84 1135 681 191 Cuts at: 5203 826 Size: 1471 Fragments arranged by size: 1471 1149 1135 837 681 219 191 84 81 SfoI GGC'GCC Cuts at: 2112 2248 2383 2112 Size: 136 135 5577 Fragments arranged by size: 5577 136 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3952 4853 5115 820 Size: 197 926 2009 901 262 1553 Fragments arranged by size: 2009 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3089 3206 1132 Size: 1957 117 3774 Fragments arranged by size: 3774 1957 117 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2223 2517 2539 3287 513 Size: 552 1158 294 22 748 3074 Fragments arranged by size: 3074 1158 748 552 294 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 2940 4094 Size: 274 80 33 51 1443 855 108 1154 Cuts at: 4094 5806 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1154 855 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2814 2846 5808 5840 2814 Size: 32 2962 32 2822 Fragments arranged by size: 2962 2822 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2449 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI