################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KasI Ksp632I KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 409 GGTACC G^GTACC AccI 55 GTCTAC GT^[AC][GT]AC AccII 60 CGCG CG^CG AcyI 244 GGCGTC G[GA]^CG[CT]C AflIII 569 ACATGT A^C[GA][CT]GT Aor51HI 89 AGCGCT AGC^GCT ApaI 821 GGGCCC GGGCC^C Asp718I 409 GGTACC G^GTACC AspHI 359 GTGCTC G[AT]GC[AT]^C AvaI 899 CCCGAG C^[CT]CG[GA]G BanI 409 GGTACC G^G[CT][GA]CC BanII 821 GGGCCC G[GA]GC[CT]^C BbrPI 493 CACGTG CAC^GTG BcgI 767 GCACACCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 712 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 26 AGATCT A^GATCT BsaAI 493 CACGTG [CT]AC^GT[GA] BsaHI 244 GGCGTC G[GA]^CG[CT]C BsaI 310 GAGACC GGTCTC(1/5) BsiHKAI 359 GTGCTC G[AT]GC[AT]^C BsoBI 899 CCCGAG C^[CT]CG[GA]G BspLU11I 569 ACATGT A^CATGT BspMI 459 GCAGGT ACCTGC(4/8) BsrDI 813 CATTGC GCAATG(2/0) BssSI 481 CTCGTG CACGAG(-5/-1) BstUI 60 CGCG CG^CG BstXI 716 CCATGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 520 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 89 AGCGCT AGC^GCT HgaI 245 GCGTC GACGC(5/10) Hin1I 244 GGCGTC G[GA]^CG[CT]C KpnI 409 GGTACC GGTAC^C MaeII 494 ACGT A^CGT MaeIII 275 GTGAC ^GT[ACGT]AC MseI 784 TTAA T^TAA MslI 717 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 60 CGCG CG^CG PmaCI 493 CACGTG CAC^GTG PmlI 493 CACGTG CAC^GTG PstI 851 CTGCAG CTGCA^G SfcI 851 CTGCAG C^T[GA][CT]AG SfiI 711 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 414 CTCAAG C^T[CT][GA]AG TaqI 795 TCGA T^CGA Tru9I 784 TTAA T^TAA Tsp45I 275 GTGAC ^GT[GC]AC TthHB8I 795 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 126 GTAC GT^AC 410 GTAC AvaII 696 GGACC G^G[AT]CC 912 GGACC BamHI 227 GGATCC G^GATCC 393 GGATCC BbsI 498 GAAGAC GAAGAC(2/6) 649 GAAGAC BbvI 110 GCAGC GCAGC(8/12) 853 GCAGC BfaI 625 CTAG C^TAG 748 CTAG BmyI 359 GTGCTC G[AGT]GC[ACT]^C 821 GGGCCC BpuAI 498 GAAGAC GAAGAC(2/6) 649 GAAGAC BsaBI 394 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 984 GATCGCCATC BseRI 121 GAGGAG GAGGAG(10/8) 160 GAGGAG BsmAI 310 GAGAC GTCTC(1/5) 451 GAGAC Bsp1286I 359 GTGCTC G[AGT]GC[ACT]^C 821 GGGCCC DdeI 971 CTCAG C^T[ACGT]AG 1018 CTCAG DsaI 716 CCATGG C^C[GA][CT]GG 722 CCATGG EcoT14I 716 CCATGG C^C[AT][AT]GG 722 CCATGG HinfI 477 GAATC G^A[ACGT]TC 744 GATTC MaeI 625 CTAG C^TAG 748 CTAG MamI 394 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 984 GATCGCCATC MboII 498 GAAGA GAAGA(8/7) 649 GAAGA NcoI 716 CCATGG C^CATGG 722 CCATGG PpuMI 695 AGGACCC [GA]G^G[AT]CC[CT] 911 AGGACCC PvuII 505 CAGCTG CAG^CTG 932 CAGCTG RsaI 126 GTAC GT^AC 410 GTAC SfaNI 660 GCATC GCATC(5/9) 964 GCATC StyI 716 CCATGG C^C[AT][AT]GG 722 CCATGG TfiI 477 GAATC G^A[AT]TC 744 GATTC TseI 110 GCAGC G^C[AT]GC 853 GCAGC XbaI 624 TCTAGA T^CTAGA 747 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AluI AlwI BalI BcnI BpmI BsaJI BsgI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI TspRI XcmI XhoII