(Circular) (Six-base) MAPSORT of: pFN21AE2278.seq Check: 6618 from: 1 to: 5731 pFN21AE2278 5731 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2455 2455 Size: 5731 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3541 278 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2061 3074 1937 Size: 124 1013 4594 Fragments arranged by size: 4594 1013 124 Acc65I G'GTAC_C Cuts at: 2414 3052 2414 Size: 638 5093 AclI AA'CG_TT Cuts at: 1563 3858 4231 1563 Size: 2295 373 3063 Fragments arranged by size: 3063 2295 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2961 3794 5178 5446 1632 Size: 1329 833 1384 268 1917 Fragments arranged by size: 1917 1384 1329 833 268 AfeI AGC'GCT Cuts at: 2096 2096 Size: 5731 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5534 AflIII A'CryG_T Cuts at: 1203 2574 4630 5607 1203 Size: 1371 2056 977 1327 Fragments arranged by size: 2056 1371 1327 977 AgeI A'CCGG_T Cuts at: 4562 4562 Size: 5731 AhdI GACnn_n'nnGTC Cuts at: 4460 4460 Size: 5731 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5731 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5731 AlwNI CAG_nnn'CTG Cuts at: 3474 5046 3474 Size: 1572 4159 ApaI G_GGCC'C Cuts at: 2830 2830 Size: 5731 ApaLI G'TGCA_C Cuts at: 3788 4944 3788 Size: 1156 4575 ApoI r'AATT_y Cuts at: 1493 3040 3266 3418 5527 1493 Size: 1547 226 152 2109 1697 Fragments arranged by size: 2109 1697 1547 226 152 AseI AT'TA_AT Cuts at: 160 1032 4285 160 Size: 872 3253 1606 Fragments arranged by size: 3253 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 AvaI C'yCGr_G Cuts at: 1870 1943 2904 3056 1870 Size: 73 961 152 4545 Fragments arranged by size: 4545 961 152 73 BamHI G'GATC_C Cuts at: 2232 2398 3061 3428 2232 Size: 166 663 367 4535 Fragments arranged by size: 4535 663 367 166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2414 3052 4507 618 Size: 325 217 627 627 638 1455 1842 Fragments arranged by size: 1842 1455 638 627 627 325 217 BanII G_rGCy'C Cuts at: 1391 2830 3050 1391 Size: 1439 220 4072 Fragments arranged by size: 4072 1439 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2510 2661 928 Size: 971 611 151 3998 Fragments arranged by size: 3998 971 611 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2761 2795 3922 3956 1903 Size: 34 824 34 1127 34 3678 Fragments arranged by size: 3678 1127 824 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3623 4833 3623 Size: 1210 4521 BclI T'GATC_A Cuts at: 1548 1548 Size: 5731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2723 4342 136 Size: 107 122 71 631 605 1051 1619 1525 Fragments arranged by size: 1619 1525 1051 631 605 122 107 71 BglII A'GATC_T Cuts at: 2031 5726 2031 Size: 3695 2036 BlpI GC'TnA_GC Cuts at: 3148 3148 Size: 5731 Bme1580I G_kGCm'C Cuts at: 1790 2830 3792 4948 1790 Size: 1040 962 1156 2573 Fragments arranged by size: 2573 1156 1040 962 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5731 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3462 4420 442 Size: 416 53 83 399 24 2045 958 1753 Fragments arranged by size: 2045 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2501 2501 Size: 5731 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2345 2474 3035 4391 1239 Size: 856 250 129 561 1356 2579 Fragments arranged by size: 2579 1356 856 561 250 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2404 3856 4721 5019 2404 Size: 1452 865 298 3116 Fragments arranged by size: 3116 1452 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 4394 882 Size: 617 810 2085 2219 Fragments arranged by size: 2219 2085 810 617 BsaAI yAC'GTr Cuts at: 493 1204 2500 3522 493 Size: 711 1296 1022 2702 Fragments arranged by size: 2702 1296 1022 711 BsaBI GATnn'nnATC Cuts at: 2403 2993 5725 2403 Size: 590 2732 2409 Fragments arranged by size: 2732 2409 590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2250 3538 3920 4555 Size: 53 83 186 936 717 1288 382 635 Cuts at: 4555 275 Size: 1451 Fragments arranged by size: 1451 1288 936 717 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4164 4562 4836 4983 5394 4164 Size: 398 274 147 411 4501 Fragments arranged by size: 4501 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2290 2320 1178 Size: 30 165 30 887 30 4589 Fragments arranged by size: 4589 887 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2180 1186 Size: 955 39 4737 Fragments arranged by size: 4737 955 39 BseYI C'CCAG_C Cuts at: 2110 2508 4934 5430 2110 Size: 398 2426 496 2411 Fragments arranged by size: 2426 2411 496 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1997 2755 2836 2981 1163 Size: 573 261 758 81 145 3913 Fragments arranged by size: 3913 758 573 261 145 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3181 3942 4970 1540 Size: 275 186 1180 761 1028 2301 Fragments arranged by size: 2301 1180 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2368 3792 3877 4948 2368 Size: 1424 85 1071 3151 Fragments arranged by size: 3151 1424 1071 85 BsmI GAATG_Cn' Cuts at: 3242 3335 3242 Size: 93 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2368 2830 3050 3792 3877 4948 1391 Size: 399 578 462 220 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 462 399 220 85 BspEI T'CCGG_A Cuts at: 5394 5394 Size: 5731 BspHI T'CATG_A Cuts at: 3620 5350 3620 Size: 1730 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2455 3086 844 Size: 1611 631 3489 Fragments arranged by size: 3489 1611 631 BsrBI CCG'CTC Cuts at: 1394 1823 3618 1394 Size: 429 1795 3507 Fragments arranged by size: 3507 1795 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2817 4226 4400 65 Size: 1183 1569 1409 174 1396 Fragments arranged by size: 1569 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4375 4562 1700 Size: 110 2565 187 2869 Fragments arranged by size: 2869 2565 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5731 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5731 BssSI C'ACGA_G Cuts at: 2486 3791 4803 2486 Size: 1305 1012 3414 Fragments arranged by size: 3414 1305 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5731 BstBI TT'CG_AA Cuts at: 4535 4535 Size: 5731 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5731 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 5731 BstYI r'GATC_y Cuts at: 1972 2031 2232 2398 3061 3428 3813 3830 5282 Size: 59 201 166 663 367 385 17 1452 Cuts at: 5282 5368 5380 5726 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 663 385 367 346 201 166 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2721 2727 513 Size: 552 1656 6 3517 Fragments arranged by size: 3517 1656 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2081 487 Size: 683 56 855 4137 Fragments arranged by size: 4137 855 683 56 BtsI GCAGTG_nn' Cuts at: 2672 2853 2969 3247 4041 4061 2672 Size: 181 116 278 794 20 4342 Fragments arranged by size: 4342 794 278 181 116 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5731 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5696 DraI TTT'AAA Cuts at: 3035 3035 Size: 5731 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2531 4738 809 Size: 507 1215 2207 1802 Fragments arranged by size: 2207 1802 1215 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 2715 2724 Size: 54 1425 50 275 18 664 221 9 Cuts at: 2724 3178 4067 5696 8 Size: 454 889 1629 43 Fragments arranged by size: 1629 1425 889 664 454 275 221 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3178 1537 Size: 275 1366 4090 Fragments arranged by size: 4090 1366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3661 1353 Size: 2308 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2612 4318 4692 4838 387 Size: 1064 138 1023 1706 374 146 1280 Fragments arranged by size: 1706 1280 1064 1023 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2345 2474 2961 3035 3794 4391 Size: 393 463 250 129 487 74 759 597 Cuts at: 4391 5178 5446 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 759 597 487 463 393 268 250 129 74 EcoO109I rG'GnC_Cy Cuts at: 2701 2827 2917 2701 Size: 126 90 5515 Fragments arranged by size: 5515 126 90 EcoRI G'AATT_C Cuts at: 3040 3418 3040 Size: 378 5353 FspI TGC'GCA Cuts at: 4237 4237 Size: 5731 HaeII r_GCGC'y Cuts at: 1678 2051 2098 2111 4878 1678 Size: 373 47 13 2767 2531 Fragments arranged by size: 2767 2531 373 47 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2793 2825 4372 4404 4446 Size: 32 135 32 1221 32 1547 32 42 Cuts at: 4446 4478 1373 Size: 32 2626 Fragments arranged by size: 2626 1547 1221 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3075 3321 669 Size: 988 281 1137 246 3079 Fragments arranged by size: 3079 1137 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5731 HpaI GTT'AAC Cuts at: 3321 3321 Size: 5731 KpnI G_GTAC'C Cuts at: 2418 3056 2418 Size: 638 5093 MfeI C'AATT_G Cuts at: 3330 3330 Size: 5731 MluI A'CGCG_T Cuts at: 5607 5607 Size: 5731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4845 5029 1305 Size: 3540 184 2007 Fragments arranged by size: 3540 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2496 2717 2726 10 Size: 54 1425 343 664 221 9 3015 Fragments arranged by size: 3015 1425 664 343 221 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2726 3689 4048 4207 518 Size: 648 1560 963 359 159 2042 Fragments arranged by size: 2042 1560 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2512 2861 2939 3147 3824 4972 5217 1606 Size: 906 349 78 208 677 1148 245 2120 Fragments arranged by size: 2120 1148 906 677 349 245 208 78 NcoI C'CATG_G Cuts at: 513 1065 2721 2727 513 Size: 552 1656 6 3517 Fragments arranged by size: 3517 1656 552 6 NdeI CA'TA_TG Cuts at: 387 387 Size: 5731 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5731 NotI GC'GGCC_GC Cuts at: 3178 3178 Size: 5731 NspI r_CATG'y Cuts at: 1132 1229 2439 2578 2808 3089 4634 1132 Size: 97 1210 139 230 281 1545 2229 Fragments arranged by size: 2229 1545 1210 281 230 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5731 PciI A'CATG_T Cuts at: 2574 4630 2574 Size: 2056 3675 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5731 PmeI GTTT'AAAC Cuts at: 3035 3035 Size: 5731 PmlI CAC'GTG Cuts at: 2500 2500 Size: 5731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3800 3832 5313 5345 3800 Size: 32 1481 32 4186 Fragments arranged by size: 4186 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2701 2917 2701 Size: 216 5515 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5731 PsiI TTA'TAA Cuts at: 3301 3301 Size: 5731 PspOMI G'GGCC_C Cuts at: 2826 2826 Size: 5731 PstI C_TGCA'G Cuts at: 830 2860 3083 830 Size: 2030 223 3478 Fragments arranged by size: 3478 2030 223 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5731 PvuII CAG'CTG Cuts at: 2512 2939 2512 Size: 427 5304 SalI G'TCGA_C Cuts at: 1936 3073 1936 Size: 1137 4594 SbfI CC_TGCA'GG Cuts at: 3083 3083 Size: 5731 ScaI AGT'ACT Cuts at: 3979 5691 3979 Size: 1712 4019 SfcI C'TryA_G Cuts at: 826 1045 2856 3079 4214 4895 5086 826 Size: 219 1811 223 1135 681 191 1471 Fragments arranged by size: 1811 1471 1135 681 223 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2723 2723 Size: 5731 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5731 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5731 SmaI CCC'GGG Cuts at: 1872 3058 1872 Size: 1186 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2419 3835 4736 4998 820 Size: 197 926 476 1416 901 262 1553 Fragments arranged by size: 1553 1416 926 901 476 262 197 SnaBI TAC'GTA Cuts at: 493 3522 493 Size: 3029 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5731 SphI G_CATG'C Cuts at: 1132 3089 1132 Size: 1957 3774 SspI AAT'ATT Cuts at: 5 52 3655 5 Size: 47 3603 2081 Fragments arranged by size: 3603 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5731 StyI C'CwwG_G Cuts at: 513 1065 2721 2727 3170 513 Size: 552 1656 6 443 3074 Fragments arranged by size: 3074 1656 552 443 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2220 2579 2685 3758 3775 3928 4113 Size: 1255 640 359 106 1073 17 153 185 Cuts at: 4113 4558 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1073 640 445 359 185 153 106 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 3977 5689 Size: 274 80 33 51 1443 153 1847 1712 Cuts at: 5689 96 Size: 138 Fragments arranged by size: 1847 1712 1443 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2290 2322 2727 2759 5691 5723 2290 Size: 32 405 32 2932 32 2298 Fragments arranged by size: 2932 2298 405 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5731 XbaI T'CTAG_A Cuts at: 2629 2752 3067 2629 Size: 123 315 5293 Fragments arranged by size: 5293 315 123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2725 2734 2449 Size: 276 9 5446 Fragments arranged by size: 5446 276 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5731 XmaI C'CCGG_G Cuts at: 1870 3056 1870 Size: 1186 4545 XmnI GAAnn'nnTTC Cuts at: 3860 3860 Size: 5731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3539 276 Size: 53 83 186 936 2005 2468 Fragments arranged by size: 2468 2005 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI