(Circular) (Six-base) MAPSORT of: pFN21AB6863.seq Check: 4321 from: 1 to: 5242 pFN21AB6863 5242 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3052 278 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 1937 Size: 648 4594 Acc65I G'GTAC_C Cuts at: 2563 2563 Size: 5242 AclI AA'CG_TT Cuts at: 1563 3369 3742 1563 Size: 1806 373 3063 Fragments arranged by size: 3063 1806 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3305 4689 4957 1632 Size: 1673 1384 268 1917 Fragments arranged by size: 1917 1673 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2531 820 Size: 197 1514 3531 Fragments arranged by size: 3531 1514 197 AflIII A'CryG_T Cuts at: 1203 2183 4141 5118 1203 Size: 980 1958 977 1327 Fragments arranged by size: 1958 1327 980 977 AgeI A'CCGG_T Cuts at: 4073 4073 Size: 5242 AhdI GACnn_n'nnGTC Cuts at: 3971 3971 Size: 5242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5242 AlwNI CAG_nnn'CTG Cuts at: 2985 4557 2985 Size: 1572 3670 ApaLI G'TGCA_C Cuts at: 3299 4455 3299 Size: 1156 4086 ApoI r'AATT_y Cuts at: 1493 2010 2551 2777 2929 5038 1493 Size: 517 541 226 152 2109 1697 Fragments arranged by size: 2109 1697 541 517 226 152 AseI AT'TA_AT Cuts at: 160 1032 2175 3796 160 Size: 872 1143 1621 1606 Fragments arranged by size: 1621 1606 1143 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 AvaI C'yCGr_G Cuts at: 1870 1943 2567 1870 Size: 73 624 4545 Fragments arranged by size: 4545 624 73 BamHI G'GATC_C Cuts at: 2572 2939 2572 Size: 367 4875 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2563 4018 618 Size: 325 217 627 776 1455 1842 Fragments arranged by size: 1842 1455 776 627 325 217 BanII G_rGCy'C Cuts at: 1391 2561 1391 Size: 1170 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3433 3467 1903 Size: 34 1496 34 3678 Fragments arranged by size: 3678 1496 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3134 4344 3134 Size: 1210 4032 BclI T'GATC_A Cuts at: 1548 1548 Size: 5242 BfrBI ATG'CAT Cuts at: 2400 2400 Size: 5242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3853 136 Size: 107 122 71 631 605 2181 1525 Fragments arranged by size: 2181 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5237 5237 Size: 5242 BlpI GC'TnA_GC Cuts at: 2659 2659 Size: 5242 Bme1580I G_kGCm'C Cuts at: 1790 3303 4459 1790 Size: 1513 1156 2573 Fragments arranged by size: 2573 1513 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5242 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2416 2973 3931 Size: 416 53 83 399 24 999 557 958 Cuts at: 3931 442 Size: 1753 Fragments arranged by size: 1753 999 958 557 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5242 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2336 2336 Size: 5242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3902 1239 Size: 2663 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3367 4232 4530 3367 Size: 865 298 4079 Fragments arranged by size: 4079 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3905 882 Size: 617 2406 2219 Fragments arranged by size: 2406 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3033 493 Size: 711 1829 2702 Fragments arranged by size: 2702 1829 711 BsaBI GATnn'nnATC Cuts at: 5236 5236 Size: 5242 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3049 3431 4066 275 Size: 53 83 186 936 1516 382 635 1451 Fragments arranged by size: 1516 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3675 4073 4347 4494 4905 3675 Size: 398 274 147 411 4012 Fragments arranged by size: 4012 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5017 Fragments arranged by size: 5017 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5242 BseYI C'CCAG_C Cuts at: 4445 4941 4445 Size: 496 4746 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4669 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2692 3453 4481 1540 Size: 275 186 691 761 1028 2301 Fragments arranged by size: 2301 1028 761 691 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 2482 3303 3388 4459 2055 Size: 427 821 85 1071 2838 Fragments arranged by size: 2838 1071 821 427 85 BsmI GAATG_Cn' Cuts at: 2402 2753 2846 2402 Size: 351 93 4798 Fragments arranged by size: 4798 351 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2482 2561 3303 3388 4459 1391 Size: 399 265 427 79 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 265 85 79 BspEI T'CCGG_A Cuts at: 4905 4905 Size: 5242 BspHI T'CATG_A Cuts at: 3131 4861 3131 Size: 1730 3512 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 844 Size: 1753 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3129 1394 Size: 429 1306 3507 Fragments arranged by size: 3507 1306 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3737 3911 65 Size: 1183 2489 174 1396 Fragments arranged by size: 2489 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3886 4073 1700 Size: 110 2076 187 2869 Fragments arranged by size: 2869 2076 187 110 BsrGI T'GTAC_A Cuts at: 96 2128 96 Size: 2032 3210 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5242 BssSI C'ACGA_G Cuts at: 3302 4314 3302 Size: 1012 4230 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 794 Size: 1683 3559 BstBI TT'CG_AA Cuts at: 4046 4046 Size: 5242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5242 BstYI r'GATC_y Cuts at: 1972 2572 2939 3324 3341 4793 4879 4891 5237 Size: 600 367 385 17 1452 86 12 346 Cuts at: 5237 1972 Size: 1977 Fragments arranged by size: 1977 1452 600 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3751 Fragments arranged by size: 3751 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4503 Fragments arranged by size: 4503 683 56 BtsI GCAGTG_nn' Cuts at: 2758 3552 3572 2758 Size: 794 20 4428 Fragments arranged by size: 4428 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5242 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5207 DraI TTT'AAA Cuts at: 2546 2546 Size: 5242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4249 809 Size: 507 2933 1802 Fragments arranged by size: 2933 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2689 3578 Size: 54 1425 50 275 18 379 480 889 Cuts at: 3578 5207 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 480 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2689 1537 Size: 275 877 4090 Fragments arranged by size: 4090 877 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2157 2226 3172 1353 Size: 668 136 69 946 3423 Fragments arranged by size: 3423 946 668 136 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3829 4203 4349 387 Size: 1064 138 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3305 3902 4689 4957 1239 Size: 393 1673 597 787 268 1524 Fragments arranged by size: 1673 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2145 2145 Size: 5242 EcoO109I rG'GnC_Cy Cuts at: 2344 2344 Size: 5242 EcoRI G'AATT_C Cuts at: 2010 2551 2929 2010 Size: 541 378 4323 Fragments arranged by size: 4323 541 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2237 2237 Size: 5242 FspI TGC'GCA Cuts at: 3748 3748 Size: 5242 HaeII r_GCGC'y Cuts at: 1678 4389 1678 Size: 2711 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373 Size: 32 135 32 2311 32 42 32 2626 Fragments arranged by size: 2626 2311 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2586 2832 669 Size: 988 281 648 246 3079 Fragments arranged by size: 3079 988 648 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5242 HpaI GTT'AAC Cuts at: 2832 2832 Size: 5242 KpnI G_GTAC'C Cuts at: 2567 2567 Size: 5242 MfeI C'AATT_G Cuts at: 2841 2841 Size: 5242 MluI A'CGCG_T Cuts at: 5118 5118 Size: 5242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4356 4540 1305 Size: 3051 184 2007 Fragments arranged by size: 3051 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2211 10 Size: 54 1425 343 379 3041 Fragments arranged by size: 3041 1425 379 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3200 3559 3718 518 Size: 648 2034 359 159 2042 Fragments arranged by size: 2042 2034 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2658 3335 4483 4728 1606 Size: 1052 677 1148 245 2120 Fragments arranged by size: 2120 1148 1052 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3751 Fragments arranged by size: 3751 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5242 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5242 NotI GC'GGCC_GC Cuts at: 2689 2689 Size: 5242 NsiI A_TGCA'T Cuts at: 2402 2402 Size: 5242 NspI r_CATG'y Cuts at: 1132 1229 2475 2600 4145 1132 Size: 97 1246 125 1545 2229 Fragments arranged by size: 2229 1545 1246 125 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5242 PciI A'CATG_T Cuts at: 4141 4141 Size: 5242 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5242 PfoI T'CCnGG_A Cuts at: 2231 2231 Size: 5242 PmeI GTTT'AAAC Cuts at: 2546 2546 Size: 5242 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3311 3343 4824 4856 3311 Size: 32 1481 32 3697 Fragments arranged by size: 3697 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2344 Size: 5242 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5242 PsiI TTA'TAA Cuts at: 2812 2812 Size: 5242 PstI C_TGCA'G Cuts at: 830 2594 830 Size: 1764 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5242 SalI G'TCGA_C Cuts at: 1936 2584 1936 Size: 648 4594 SbfI CC_TGCA'GG Cuts at: 2594 2594 Size: 5242 ScaI AGT'ACT Cuts at: 3490 5202 3490 Size: 1712 3530 SfcI C'TryA_G Cuts at: 826 1045 2406 2590 3725 4406 4597 826 Size: 219 1361 184 1135 681 191 1471 Fragments arranged by size: 1471 1361 1135 681 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5242 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5242 SmaI CCC'GGG Cuts at: 1872 2569 1872 Size: 697 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 3346 4247 4509 820 Size: 197 926 588 815 901 262 1553 Fragments arranged by size: 1553 926 901 815 588 262 197 SnaBI TAC'GTA Cuts at: 493 3033 493 Size: 2540 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5242 SphI G_CATG'C Cuts at: 1132 2475 2600 1132 Size: 1343 125 3774 Fragments arranged by size: 3774 1343 125 SspI AAT'ATT Cuts at: 5 52 3166 5 Size: 47 3114 2081 Fragments arranged by size: 3114 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5242 StyI C'CwwG_G Cuts at: 513 1065 2004 2503 2681 513 Size: 552 939 499 178 3074 Fragments arranged by size: 3074 939 552 499 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3269 3286 3439 3624 4069 325 Size: 1255 1689 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2128 2282 3488 Size: 274 80 33 51 1443 151 154 1206 Cuts at: 3488 5200 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1206 274 154 151 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5202 5234 5202 Size: 32 5210 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5242 XbaI T'CTAG_A Cuts at: 2578 2578 Size: 5242 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5242 XmaI C'CCGG_G Cuts at: 1870 2567 1870 Size: 697 4545 XmnI GAAnn'nnTTC Cuts at: 2220 3371 2220 Size: 1151 4091 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3050 276 Size: 53 83 186 936 1516 2468 Fragments arranged by size: 2468 1516 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI