################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MboI MfeI MflI MluI MspA1I MunI MvnI NaeI NarI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI StyI SwaI Tth111I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 65 TCCGGA T^CCGGA ApaI 148 GGGCCC GGGCC^C AspHI 402 GAGCTC G[AT]GC[AT]^C AvaI 956 CTCGAG C^[CT]CG[GA]G AvaII 63 GGTCC G^G[AT]CC BalI 1073 TGGCCA TGG^CCA BbsI 580 GAAGAC GAAGAC(2/6) BcgI 848 GCACAGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 396 CCGGG CC^[GC]GG BfaI 56 CTAG C^TAG BpuAI 580 GAAGAC GAAGAC(2/6) BsaAI 787 TACGTG [CT]AC^GT[GA] BsaI 959 GAGACC GGTCTC(1/5) BsaWI 65 TCCGGA [AT]^CCGG[AT] BseAI 65 TCCGGA T^CCGGA BsiHKAI 402 GAGCTC G[AT]GC[AT]^C BsoBI 956 CTCGAG C^[CT]CG[GA]G Bsp1407I 927 TGTACA T^GTACA BspEI 65 TCCGGA T^CCGGA BsrGI 927 TGTACA T^GTACA BstAPI 1126 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 678 GCATGC GC[ACGT]^[ACGT]GC DraI 166 TTTAAA TTT^AAA EaeI 1073 TGGCCA [CT]^GGCC[GA] EcoNI 561 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 271 GAATTC G^AATTC EcoRV 208 GATATC GAT^ATC FauI 407 CCCGC CCCGC(4/6) MaeI 56 CTAG C^TAG MluNI 1073 TGGCCA TGG^CCA MroI 65 TCCGGA T^CCGGA MscI 1073 TGGCCA TGG^CCA NciI 396 CCGGG CC^[GC]GG NdeI 182 CATATG CA^TATG NspI 678 GCATGC [GA]CATG^[CT] PaeR7I 956 CTCGAG C^TCGAG PleI 141 GACTC GAGTC(4/5) SacI 402 GAGCTC GAGCT^C SapI 700 GCTCTTC GCTCTTC(1/4) SphI 678 GCATGC GCATG^C SspBI 927 TGTACA T^GTACA XhoI 956 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 390 CCGC CCGC(-3/-1) 408 CCGC BanII 148 GGGCCC G[GA]GC[CT]^C 402 GAGCTC BbvCI 287 CCTCAGC CCTCAGC(-5/-2) 695 CCTCAGC BmyI 148 GGGCCC G[AGT]GC[ACT]^C 402 GAGCTC Bsp1286I 148 GGGCCC G[AGT]GC[ACT]^C 402 GAGCTC BspMI 764 ACCTGC ACCTGC(4/8) 938 ACCTGC EarI 701 CTCTTC CTCTTC(1/4) 812 GAAGAG HapII 66 CCGG C^CGG 396 CCGG HpaII 66 CCGG C^CGG 396 CCGG Ksp632I 701 CTCTTC CTCTTC(1/4) 812 GAAGAG MaeII 746 ACGT A^CGT 788 ACGT MslI 197 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 636 CACCCAGGTG MspI 66 CCGG C^CGG 396 CCGG MwoI 1075 GCCAAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1126 GCAGCATTTGC PflMI 181 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 445 CCACAGTATGG TaqI 669 TCGA T^CGA 957 TCGA TspRI 627 CACTG CA[GC]TG[ACGT][ACGT]^ 1005 CAGTG TthHB8I 669 TCGA T^CGA 957 TCGA Van91I 181 CCATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 445 CCACAGTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboII MnlI MseI MvaI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp45I Tsp509I