################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KasI MaeI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 464 GGTACC G^GTACC AlwI 1080 GGATC GGATC(4/5) ApaI 374 GGGCCC GGGCC^C Asp718I 464 GGTACC G^GTACC AvaI 114 CTCGGG C^[CT]CG[GA]G BalI 161 TGGCCA TGG^CCA BsaBI 159 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 350 CGGCCG CG[GA][CT]^CG BsmI 760 GCATTC GAATGC(1/-1) BsoBI 114 CTCGGG C^[CT]CG[GA]G BsrFI 461 GCCGGT [GA]^CCGG[CT] BsrI 951 ACTGG ACTGG(1/-1) BssSI 874 CACGAG CACGAG(-5/-1) Cfr10I 461 GCCGGT [GA]^CCGG[CT] DpnII 1081 GATC ^GATC EagI 350 CGGCCG C^GGCCG EclXI 350 CGGCCG C^GGCCG Eco52I 350 CGGCCG C^GGCCG HaeII 39 GGCGCT [GA]GCGC^[CT] KpnI 464 GGTACC GGTAC^C KspI 170 CCGCGG CCGC^GG MamI 159 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 1081 GATC ^GATC MluNI 161 TGGCCA TGG^CCA MscI 161 TGGCCA TGG^CCA MslI 819 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 818 CCATGG C^CATGG NdeII 1081 GATC ^GATC NspI 443 GCATGC [GA]CATG^[CT] PflMI 831 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 805 GGGACCC [GA]G^G[AT]CC[CT] SacI 964 GAGCTC GAGCT^C SacII 170 CCGCGG CCGC^GG Sau3AI 1081 GATC ^GATC SphI 443 GCATGC GCATG^C Tsp509I 121 AATT ^AATT Van91I 831 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 641 CCATCTCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 221 GGCGTC G[GA]^CG[CT]C 604 GACGCC AfaI 465 GTAC GT^AC 1008 GTAC BanI 464 GGTACC G^G[CT][GA]CC 568 GGTGCC BbrPI 519 CACGTG CAC^GTG 880 CACGTG BcnI 48 CCCGG CC^[GC]GG 125 CCCGG BsaAI 519 CACGTG [CT]AC^GT[GA] 880 CACGTG BsaHI 221 GGCGTC G[GA]^CG[CT]C 604 GACGCC BsmFI 486 GGGAC GGGAC(10/14) 805 GGGAC BstF5I 543 CATCC GGATG(2/0) 824 GGATG BstXI 432 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 818 CCATGGGGATGG DsaI 170 CCGCGG C^C[GA][CT]GG 818 CCATGG EcoT14I 144 CCAAGG C^C[AT][AT]GG 818 CCATGG FokI 543 CATCC GGATG(9/13) 824 GGATG Hin1I 221 GGCGTC G[GA]^CG[CT]C 604 GACGCC HphI 906 GGTGA GGTGA(8/7) 167 TCACC MaeIII 309 GTCAC ^GT[ACGT]AC 907 GTGAC NciI 48 CCCGG CC^[GC]GG 125 CCCGG PmaCI 519 CACGTG CAC^GTG 880 CACGTG PmlI 519 CACGTG CAC^GTG 880 CACGTG RsaI 465 GTAC GT^AC 1008 GTAC SmlI 15 CTCAAG C^T[CT][GA]AG 745 CTCAAG StyI 144 CCAAGG C^C[AT][AT]GG 818 CCATGG Tsp45I 309 GTCAC ^GT[GC]AC 907 GTGAC TspRI 226 CAGTG CA[GC]TG[ACGT][ACGT]^ 327 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaII BanII BbvI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI Bsp1286I BspMI BssKI BstAPI BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI Ksp632I MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV PstI PvuII SapI Sau96I ScrFI SfcI TseI