(Circular) (Six-base) MAPSORT of: pFN21AE0787.seq Check: 3836 from: 1 to: 5764 pFN21AE0787 5764 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 2189 Size: 5764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 3827 5211 5479 1632 Size: 614 1581 1384 268 1917 Fragments arranged by size: 1917 1581 1384 614 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2102 2747 1166 Size: 936 645 4183 Fragments arranged by size: 4183 936 645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 2344 2644 3507 5079 2344 Size: 300 863 1572 3029 Fragments arranged by size: 3029 1572 863 300 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 3073 3299 3451 5560 1493 Size: 1580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 2268 3089 1870 Size: 73 325 821 4545 Fragments arranged by size: 4545 821 325 73 BamHI G'GATC_C Cuts at: 2154 2824 3094 3461 2154 Size: 670 270 367 4457 Fragments arranged by size: 4457 670 367 270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 3083 1391 Size: 1692 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2234 928 Size: 971 335 4458 Fragments arranged by size: 4458 971 335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3024 3181 3024 Size: 157 5607 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2946 3495 4453 Size: 416 53 83 399 24 1529 549 958 Cuts at: 4453 442 Size: 1753 Fragments arranged by size: 1753 1529 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2276 2558 4424 1239 Size: 974 63 282 1866 2579 Fragments arranged by size: 2579 1866 974 282 63 Bpu10I CC'TnA_GC Cuts at: 2720 2720 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2006 2138 3889 4754 5052 2006 Size: 132 1751 865 298 2718 Fragments arranged by size: 2718 1751 865 298 132 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2426 4197 4595 4869 5016 5427 2426 Size: 1771 398 274 147 411 2763 Fragments arranged by size: 2763 1771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2288 2479 2585 1186 Size: 1018 84 191 106 4365 Fragments arranged by size: 4365 1018 191 106 84 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2155 2330 1163 Size: 573 419 175 4597 Fragments arranged by size: 4597 573 419 175 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2210 3214 3975 5003 1540 Size: 275 186 209 1004 761 1028 2301 Fragments arranged by size: 2301 1028 1004 761 275 209 186 BsiHKAI G_wGCw'C Cuts at: 2022 2726 3825 3910 4981 2022 Size: 704 1099 85 1071 2805 Fragments arranged by size: 2805 1099 1071 704 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2726 3083 3825 3910 4981 1391 Size: 399 232 704 357 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 704 399 357 232 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 3119 844 Size: 1345 930 3489 Fragments arranged by size: 3489 1345 930 BsrBI CCG'CTC Cuts at: 1394 1823 2212 2278 3651 1394 Size: 429 389 66 1373 3507 Fragments arranged by size: 3507 1373 429 389 66 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 3196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2903 3824 4836 2903 Size: 921 1012 3831 Fragments arranged by size: 3831 1012 921 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 2545 1184 Size: 1361 4403 BstXI CCAn_nnnn'nTGG Cuts at: 2536 2566 2536 Size: 30 5734 BstYI r'GATC_y Cuts at: 1972 2154 2626 2824 3094 3461 3846 3863 5315 Size: 182 472 198 270 367 385 17 1452 Cuts at: 5315 5401 5413 5759 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 472 385 367 346 270 198 182 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2582 2582 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 513 Size: 552 939 237 4036 Fragments arranged by size: 4036 939 552 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 2378 487 Size: 683 56 863 289 3873 Fragments arranged by size: 3873 863 683 289 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5729 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2526 2631 2766 Size: 54 1425 50 275 18 696 105 135 Cuts at: 2766 3211 4100 5729 8 Size: 445 889 1629 43 Fragments arranged by size: 1629 1425 889 696 445 275 135 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2856 2895 2972 3694 1353 Size: 1503 39 77 722 3423 Fragments arranged by size: 3423 1503 722 77 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2246 2276 2558 3827 4424 5211 Size: 393 581 33 30 282 1269 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 1269 787 597 581 393 282 268 33 30 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 4911 1678 Size: 3233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 2199 2231 4405 Size: 32 135 32 495 32 100 32 2174 Cuts at: 4405 4437 4479 4511 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2174 495 135 100 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 2114 748 Size: 1366 4398 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2108 2538 4878 5062 1305 Size: 803 430 2340 184 2007 Fragments arranged by size: 2340 2007 803 430 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2768 10 Size: 54 1425 343 936 3006 Fragments arranged by size: 3006 1425 936 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 2564 2747 2844 3722 4081 4240 Size: 648 936 462 183 97 878 359 159 Cuts at: 4240 518 Size: 2042 Fragments arranged by size: 2042 936 878 648 462 359 183 159 97 MspA1I CmG'CkG Cuts at: 1606 2344 3180 3857 5005 5250 1606 Size: 738 836 677 1148 245 2120 Fragments arranged by size: 2120 1148 836 738 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2879 1108 Size: 1771 3993 PfoI T'CCnGG_A Cuts at: 2160 2253 2643 2160 Size: 93 390 5281 Fragments arranged by size: 5281 390 93 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2344 2344 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 2249 4012 5724 2249 Size: 1763 1712 2289 Fragments arranged by size: 2289 1763 1712 SfcI C'TryA_G Cuts at: 826 1045 3112 4247 4928 5119 826 Size: 219 2067 1135 681 191 1471 Fragments arranged by size: 2067 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 2117 2268 3868 4769 5031 Size: 197 926 78 96 151 1600 901 262 Cuts at: 5031 820 Size: 1553 Fragments arranged by size: 1600 1553 926 901 262 197 151 96 78 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2261 2587 1517 Size: 744 326 4694 Fragments arranged by size: 4694 744 326 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 2703 3203 513 Size: 552 939 141 558 500 3074 Fragments arranged by size: 3074 939 558 552 500 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 2867 3791 3808 3961 4146 4591 Size: 1255 1002 285 924 17 153 185 445 Cuts at: 4591 325 Size: 1498 Fragments arranged by size: 1498 1255 1002 924 445 285 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2247 2664 Size: 274 80 33 51 1443 129 141 417 Cuts at: 2664 4010 5722 96 Size: 1346 1712 138 Fragments arranged by size: 1712 1443 1346 417 274 141 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 2268 1943 Size: 325 5439 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 2434 3893 2434 Size: 1459 4305 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI