Restriction map for
pFN21AB9139
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HindIII
HpaI KasI MaeI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SbfI ScaI SfcI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 611 GGTACC G^GTACC
AcyI 22 GACGCC G[GA]^CG[CT]C
AflIII 989 ACACGT A^C[GA][CT]GT
AhdI 88 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 256 CCTCAGG CC^T[ACGT]AGG
ApaI 1134 GGGCCC GGGCC^C
Asp700I 987 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 611 GGTACC G^GTACC
AspEI 88 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 40 CCCGAG C^[CT]CG[GA]G
BcgI 1364 GCAGCACCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 653 TGATCA T^GATCA
BglII 1091 AGATCT A^GATCT
BsaHI 22 GACGCC G[GA]^CG[CT]C
BsaI 1324 GAGACC GGTCTC(1/5)
BseRI 76 GAGGAG GAGGAG(10/8)
BsiEI 447 CGGCCG CG[GA][CT]^CG
BsoBI 40 CCCGAG C^[CT]CG[GA]G
BspMI 1412 ACCTGC ACCTGC(4/8)
BstYI 1091 AGATCT [GA]^GATC[CT]
Bsu36I 256 CCTCAGG CC^T[ACGT]AGG
DdeI 257 CTCAG C^T[ACGT]AG
DrdI 745 GACGTGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 447 CGGCCG C^GGCCG
Eam1105I 88 GACGGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 447 CGGCCG C^GGCCG
Eco52I 447 CGGCCG C^GGCCG
Eco81I 256 CCTCAGG CC^T[ACGT]AGG
FbaI 653 TGATCA T^GATCA
Hin1I 22 GACGCC G[GA]^CG[CT]C
HincII 469 GTTGAC GT[CT]^[GA]AC
HindII 469 GTTGAC GT[CT]^[GA]AC
KpnI 611 GGTACC GGTAC^C
MflI 1091 AGATCT [GA]^GATC[CT]
MseI 196 TTAA T^TAA
SapI 204 GCTCTTC GCTCTTC(1/4)
SexAI 1034 ACCTGGT A^CC[AT]GGT
SfiI 1158 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 793 CTTGAG C^T[CT][GA]AG
StuI 1142 AGGCCT AGG^CCT
Tru9I 196 TTAA T^TAA
XhoII 1091 AGATCT [GA]^GATC[CT]
XmnI 987 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 151 GTCTAC GT^[AC][GT]AC
400 GTCTAC
BalI 293 TGGCCA TGG^CCA
1079 TGGCCA
BanII 185 GAGCCC G[GA]GC[CT]^C
1134 GGGCCC
BglI 84 GCCTGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1159 GCCATCACGGC
BstAPI 696 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
770 GCATCTACTGC
BstXI 296 CCAGTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
364 CCAGCTTACTGG
HaeII 1266 GGCGCT [GA]GCGC^[CT]
1286 GGCGCT
KspI 34 CCGCGG CCGC^GG
56 CCGCGG
MaeIII 235 GTGAC ^GT[ACGT]AC
325 GTCAC
MluNI 293 TGGCCA TGG^CCA
1079 TGGCCA
MscI 293 TGGCCA TGG^CCA
1079 TGGCCA
PvuII 700 CAGCTG CAG^CTG
894 CAGCTG
SacII 34 CCGCGG CCGC^GG
56 CCGCGG
TaqI 671 TCGA T^CGA
908 TCGA
Tsp45I 235 GTGAC ^GT[GC]AC
325 GTCAC
TthHB8I 671 TCGA T^CGA
908 TCGA
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI