(Circular) (Six-base) MAPSORT of: pFN21AB9139.seq Check: 2590 from: 1 to: 6166 pFN21AB9139 6166 bp With 182 enzymes: SgfI * November 15, 2013 16:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 2406 3509 1937 Size: 220 249 1103 4594 Fragments arranged by size: 4594 1103 249 220 Acc65I G'GTAC_C Cuts at: 2616 3487 2616 Size: 871 5295 AclI AA'CG_TT Cuts at: 1563 3465 4293 4666 1563 Size: 1902 828 373 3063 Fragments arranged by size: 3063 1902 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2701 2834 3026 4229 5613 5881 1632 Size: 1010 59 133 192 1203 1384 268 1917 Fragments arranged by size: 1917 1384 1203 1010 268 192 133 59 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 2994 5065 6042 1203 Size: 1791 2071 977 1327 Fragments arranged by size: 2071 1791 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 2098 4895 2098 Size: 2797 3369 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AlwNI CAG_nnn'CTG Cuts at: 3909 5481 3909 Size: 1572 4594 ApaI G_GGCC'C Cuts at: 3143 3143 Size: 6166 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 3475 3701 3853 5962 1493 Size: 1982 226 152 2109 1697 Fragments arranged by size: 2109 1982 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2045 3491 1870 Size: 73 102 1446 4545 Fragments arranged by size: 4545 1446 102 73 BamHI G'GATC_C Cuts at: 3496 3863 3496 Size: 367 5799 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2168 2616 3487 4942 Size: 325 217 627 269 112 448 871 1455 Cuts at: 4942 618 Size: 1842 Fragments arranged by size: 1842 1455 871 627 448 325 269 217 112 BanII G_rGCy'C Cuts at: 1391 2194 3143 3485 1391 Size: 803 949 342 4072 Fragments arranged by size: 4072 949 803 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2630 2798 2822 3052 3420 928 Size: 971 731 168 24 230 368 3674 Fragments arranged by size: 3674 971 731 368 230 168 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3358 3392 4357 4391 1903 Size: 34 50 34 1337 34 965 34 3678 Fragments arranged by size: 3678 1337 965 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 2658 1548 Size: 1110 5056 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2095 3170 4777 Size: 107 122 71 631 605 423 1075 1607 Cuts at: 4777 136 Size: 1525 Fragments arranged by size: 1607 1525 1075 631 605 423 122 107 71 BglII A'GATC_T Cuts at: 3096 6161 3096 Size: 3065 3101 BlpI GC'TnA_GC Cuts at: 3583 3583 Size: 6166 Bme1580I G_kGCm'C Cuts at: 1790 2061 3143 4227 5383 1790 Size: 271 1082 1084 1156 2573 Fragments arranged by size: 2573 1156 1084 1082 271 BmgBI CAC'GTC Cuts at: 1318 2752 1318 Size: 1434 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3238 3410 3897 Size: 416 53 83 399 24 1821 172 487 Cuts at: 3897 4855 442 Size: 958 1753 Fragments arranged by size: 1821 1753 958 487 416 399 172 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2270 2398 2705 2717 2914 4826 1239 Size: 860 171 128 307 12 197 1912 2579 Fragments arranged by size: 2579 1912 860 307 197 171 128 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 4291 5156 5454 2819 Size: 1472 865 298 3531 Fragments arranged by size: 3531 1472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3323 4829 882 Size: 617 1824 1506 2219 Fragments arranged by size: 2219 1824 1506 617 BsaAI yAC'GTr Cuts at: 493 1204 3957 493 Size: 711 2753 2702 Fragments arranged by size: 2753 2702 711 BsaBI GATnn'nnATC Cuts at: 6160 6160 Size: 6166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 3973 4355 4990 Size: 53 83 186 936 495 1945 382 635 Cuts at: 4990 275 Size: 1451 Fragments arranged by size: 1945 1451 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4599 4997 5271 5418 5829 4599 Size: 398 274 147 411 4936 Fragments arranged by size: 4936 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3320 3350 1178 Size: 30 165 30 1917 30 3994 Fragments arranged by size: 3994 1917 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 1186 Size: 910 5256 BseYI C'CCAG_C Cuts at: 2485 2770 2901 3136 3421 5369 5865 2485 Size: 285 131 235 285 1948 496 2786 Fragments arranged by size: 2786 1948 496 285 285 235 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5593 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2455 3616 4377 5405 1540 Size: 275 186 454 1161 761 1028 2301 Fragments arranged by size: 2301 1161 1028 761 454 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2703 2757 3036 3454 4227 4312 5383 2211 Size: 492 54 279 418 773 85 1071 2994 Fragments arranged by size: 2994 1071 773 492 418 279 85 54 BsmI GAATG_Cn' Cuts at: 3677 3770 3677 Size: 93 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2061 2194 2211 2703 2757 3036 3143 Size: 399 271 133 17 492 54 279 107 Cuts at: 3143 3454 3485 4227 4312 5383 1391 Size: 311 31 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 311 279 271 133 107 85 54 31 17 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 4055 5785 4055 Size: 1730 4436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3426 3521 844 Size: 2582 95 3489 Fragments arranged by size: 3489 2582 95 BsrBI CCG'CTC Cuts at: 1394 1823 4053 1394 Size: 429 2230 3507 Fragments arranged by size: 3507 2230 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4661 4835 65 Size: 1183 3413 174 1396 Fragments arranged by size: 3413 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 2454 2923 4810 4997 1700 Size: 110 295 349 469 1887 187 2869 Fragments arranged by size: 2869 1887 469 349 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 4226 5238 4226 Size: 1012 5154 BstAPI GCAn_nnn'nTGC Cuts at: 794 2707 2781 794 Size: 1913 74 4179 Fragments arranged by size: 4179 1913 74 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2308 2376 2308 Size: 68 6098 BstYI r'GATC_y Cuts at: 1972 3096 3496 3863 4248 4265 5717 5803 5815 Size: 1124 400 367 385 17 1452 86 12 Cuts at: 5815 6161 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1124 400 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2262 2262 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 2039 2061 3174 513 Size: 552 974 22 1113 3505 Fragments arranged by size: 3505 1113 974 552 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 2495 3682 4476 4496 2495 Size: 1187 794 20 4165 Fragments arranged by size: 4165 1187 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 5173 809 Size: 507 1440 2417 1802 Fragments arranged by size: 2417 1802 1440 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2298 2452 Size: 54 1425 50 275 18 277 191 154 Cuts at: 2452 2949 3084 3162 3171 3435 3613 4502 6131 Size: 497 135 78 9 264 178 889 1629 Cuts at: 6131 8 Size: 43 Fragments arranged by size: 1629 1425 889 497 277 275 264 191 178 154 135 78 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2452 3613 1537 Size: 275 640 1161 4090 Fragments arranged by size: 4090 1161 640 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2438 2864 4096 1353 Size: 863 222 426 1232 3423 Fragments arranged by size: 3423 1232 863 426 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2270 2398 2642 2701 2705 2717 Size: 393 467 171 128 244 59 4 12 Cuts at: 2717 2834 2914 3026 4229 4826 5613 5881 1239 Size: 117 80 112 1203 597 787 268 1524 Fragments arranged by size: 1524 1203 787 597 467 393 268 244 171 128 117 112 80 59 12 4 EcoO109I rG'GnC_Cy Cuts at: 2070 2833 3425 2070 Size: 763 592 4811 Fragments arranged by size: 4811 763 592 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 3275 3295 5313 1678 Size: 1597 20 2018 2531 Fragments arranged by size: 2531 2018 1597 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2792 2824 4807 Size: 32 135 32 512 32 676 32 1983 Cuts at: 4807 4839 4881 4913 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1983 676 512 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2476 3510 3756 669 Size: 988 281 538 1034 246 3079 Fragments arranged by size: 3079 1034 988 538 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KpnI G_GTAC'C Cuts at: 2620 3491 2620 Size: 871 5295 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 2873 5280 5464 1305 Size: 1257 311 2407 184 2007 Fragments arranged by size: 2407 2007 1257 311 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2300 3086 10 Size: 54 1425 343 468 786 3090 Fragments arranged by size: 3090 1425 786 468 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2666 2750 2969 3191 4124 4483 4642 Size: 648 1500 84 219 222 933 359 159 Cuts at: 4642 518 Size: 2042 Fragments arranged by size: 2042 1500 933 648 359 222 219 159 84 MspA1I CmG'CkG Cuts at: 1606 2041 2063 2707 2901 3582 4259 5407 5652 Size: 435 22 644 194 681 677 1148 245 Cuts at: 5652 1606 Size: 2120 Fragments arranged by size: 2120 1148 681 677 644 435 245 194 22 NaeI GCC'GGC Cuts at: 2107 2456 2925 2107 Size: 349 469 5348 Fragments arranged by size: 5348 469 349 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5614 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NgoMIV G'CCGG_C Cuts at: 2105 2454 2923 2105 Size: 349 469 5348 Fragments arranged by size: 5348 469 349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 3524 5069 1132 Size: 97 2295 1545 2229 Fragments arranged by size: 2295 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6166 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6166 PfoI T'CCnGG_A Cuts at: 2445 2682 2445 Size: 237 5929 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PspOMI G'GGCC_C Cuts at: 3139 3139 Size: 6166 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2984 3016 2984 Size: 32 6134 PstI C_TGCA'G Cuts at: 830 3518 830 Size: 2688 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2707 2901 2707 Size: 194 5972 SacII CC_GC'GG Cuts at: 2042 2064 2042 Size: 22 6144 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SapI GCTCTTCn'nnn_ Cuts at: 2216 2216 Size: 6166 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SexAI A'CCwGG_T Cuts at: 3039 3039 Size: 6166 SfcI C'TryA_G Cuts at: 826 1045 3514 4649 5330 5521 826 Size: 219 2469 1135 681 191 1471 Fragments arranged by size: 2469 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3170 3170 Size: 6166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3493 1872 Size: 1621 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2798 4270 5171 5433 820 Size: 197 926 855 1472 901 262 1553 Fragments arranged by size: 1553 1472 926 901 855 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 4090 5 Size: 47 4038 2081 Fragments arranged by size: 4038 2081 47 StuI AGG'CCT Cuts at: 1517 3149 1517 Size: 1632 4534 StyI C'CwwG_G Cuts at: 513 1065 3605 513 Size: 552 2540 3074 Fragments arranged by size: 3074 2540 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3065 3411 4193 4210 4363 4548 4993 Size: 1255 1485 346 782 17 153 185 445 Cuts at: 4993 325 Size: 1498 Fragments arranged by size: 1498 1485 1255 782 445 346 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2688 4412 6124 Size: 274 80 33 51 1443 711 1724 1712 Cuts at: 6124 96 Size: 138 Fragments arranged by size: 1724 1712 1443 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3320 3352 6126 6158 3320 Size: 32 2774 32 3328 Fragments arranged by size: 3328 2774 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6166 XmaI C'CCGG_G Cuts at: 1870 3491 1870 Size: 1621 4545 XmnI GAAnn'nnTTC Cuts at: 2996 4295 2996 Size: 1299 4867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SanDI SfoI SrfI SwaI XcmI