(Linear) (Six-base) MAPSORT of: KIBB9139.seq Check: 8035 from: 1 to: 1461 KIBB9139.orf With 182 enzymes: SgfI * November 15, 2013 14:41 .. AccI GT'mk_AC Cuts at: 0 152 401 1461 Size: 152 249 1060 Fragments arranged by size: 1060 249 152 Acc65I G'GTAC_C Cuts at: 0 611 1461 Size: 611 850 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 696 829 1021 1461 Size: 637 59 133 192 440 Fragments arranged by size: 637 440 192 133 59 AflIII A'CryG_T Cuts at: 0 989 1461 Size: 989 472 AhdI GACnn_n'nnGTC Cuts at: 0 93 1461 Size: 93 1368 ApaI G_GGCC'C Cuts at: 0 1138 1461 Size: 1138 323 AvaI C'yCGr_G Cuts at: 0 40 1461 Size: 40 1421 BanI G'GyrC_C Cuts at: 0 51 163 611 1461 Size: 51 112 448 850 Fragments arranged by size: 850 448 112 51 BanII G_rGCy'C Cuts at: 0 189 1138 1461 Size: 189 949 323 Fragments arranged by size: 949 323 189 BbsI GAAGACnn'nnnn_ Cuts at: 0 625 793 817 1047 1415 1461 Size: 625 168 24 230 368 46 Fragments arranged by size: 625 368 230 168 46 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1353 1387 1461 Size: 1353 34 74 Fragments arranged by size: 1353 74 34 BclI T'GATC_A Cuts at: 0 653 1461 Size: 653 808 BglI GCCn_nnn'nGGC Cuts at: 0 90 1165 1461 Size: 90 1075 296 Fragments arranged by size: 1075 296 90 BglII A'GATC_T Cuts at: 0 1091 1461 Size: 1091 370 Bme1580I G_kGCm'C Cuts at: 0 56 1138 1461 Size: 56 1082 323 Fragments arranged by size: 1082 323 56 BmgBI CAC'GTC Cuts at: 0 747 1461 Size: 747 714 BmrI ACTGGGnnnn_n' Cuts at: 0 1233 1405 1461 Size: 1233 172 56 Fragments arranged by size: 1233 172 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 265 393 700 712 909 1461 Size: 94 171 128 307 12 197 552 Fragments arranged by size: 552 307 197 171 128 94 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 814 1461 Size: 814 647 BsaI GGTCTCn'nnnn_ Cuts at: 0 1318 1461 Size: 1318 143 BsaHI Gr'CG_yC Cuts at: 0 23 1461 Size: 23 1438 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1315 1345 1461 Size: 1315 30 116 Fragments arranged by size: 1315 116 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 1461 Size: 91 1370 BseYI C'CCAG_C Cuts at: 0 480 765 896 1131 1416 1461 Size: 480 285 131 235 285 45 Fragments arranged by size: 480 285 285 235 131 45 BsiEI CG_ry'CG Cuts at: 0 450 1461 Size: 450 1011 BsiHKAI G_wGCw'C Cuts at: 0 206 698 752 1031 1449 1461 Size: 206 492 54 279 418 12 Fragments arranged by size: 492 418 279 206 54 12 Bsp1286I G_dGCh'C Cuts at: 0 56 189 206 698 752 1031 1138 1449 Size: 56 133 17 492 54 279 107 311 Cuts at: 1449 1461 Size: 12 Fragments arranged by size: 492 311 279 133 107 56 54 17 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1421 1461 Size: 1421 40 BsrFI r'CCGG_y Cuts at: 0 100 449 918 1461 Size: 100 349 469 543 Fragments arranged by size: 543 469 349 100 BstAPI GCAn_nnn'nTGC Cuts at: 0 702 776 1461 Size: 702 74 685 Fragments arranged by size: 702 685 74 BstXI CCAn_nnnn'nTGG Cuts at: 0 303 371 1461 Size: 303 68 1090 Fragments arranged by size: 1090 303 68 BstYI r'GATC_y Cuts at: 0 1091 1461 Size: 1091 370 Bsu36I CC'TnA_GG Cuts at: 0 257 1461 Size: 257 1204 BtgI C'CryG_G Cuts at: 0 34 56 1169 1461 Size: 34 22 1113 292 Fragments arranged by size: 1113 292 34 22 BtsI GCAGTG_nn' Cuts at: 0 490 1461 Size: 490 971 DrdI GACnn_nn'nnGTC Cuts at: 0 751 1461 Size: 751 710 EaeI y'GGCC_r Cuts at: 0 102 293 447 944 1079 1157 1166 1430 Size: 102 191 154 497 135 78 9 264 Cuts at: 1430 1461 Size: 31 Fragments arranged by size: 497 264 191 154 135 102 78 31 9 EagI C'GGCC_G Cuts at: 0 447 1461 Size: 447 1014 EarI CTCTTCn'nnn_ Cuts at: 0 211 433 859 1461 Size: 211 222 426 602 Fragments arranged by size: 602 426 222 211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 94 265 393 637 696 700 712 829 Size: 94 171 128 244 59 4 12 117 Cuts at: 829 909 1021 1461 Size: 80 112 440 Fragments arranged by size: 440 244 171 128 117 112 94 80 59 12 4 EcoO109I rG'GnC_Cy Cuts at: 0 65 828 1420 1461 Size: 65 763 592 41 Fragments arranged by size: 763 592 65 41 HaeII r_GCGC'y Cuts at: 0 1270 1290 1461 Size: 1270 20 171 Fragments arranged by size: 1270 171 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 787 819 1461 Size: 79 32 676 32 642 Fragments arranged by size: 676 642 79 32 32 HincII GTy'rAC Cuts at: 0 471 1461 Size: 471 990 KpnI G_GTAC'C Cuts at: 0 615 1461 Size: 615 846 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 557 868 1461 Size: 557 311 593 Fragments arranged by size: 593 557 311 MscI TGG'CCA Cuts at: 0 295 1081 1461 Size: 295 786 380 Fragments arranged by size: 786 380 295 MslI CAynn'nnrTG Cuts at: 0 661 745 964 1186 1461 Size: 661 84 219 222 275 Fragments arranged by size: 661 275 222 219 84 MspA1I CmG'CkG Cuts at: 0 36 58 702 896 1461 Size: 36 22 644 194 565 Fragments arranged by size: 644 565 194 36 22 NaeI GCC'GGC Cuts at: 0 102 451 920 1461 Size: 102 349 469 541 Fragments arranged by size: 541 469 349 102 NgoMIV G'CCGG_C Cuts at: 0 100 449 918 1461 Size: 100 349 469 543 Fragments arranged by size: 543 469 349 100 PfoI T'CCnGG_A Cuts at: 0 440 677 1461 Size: 440 237 784 Fragments arranged by size: 784 440 237 PspOMI G'GGCC_C Cuts at: 0 1134 1461 Size: 1134 327 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 979 1011 1461 Size: 979 32 450 Fragments arranged by size: 979 450 32 PvuII CAG'CTG Cuts at: 0 702 896 1461 Size: 702 194 565 Fragments arranged by size: 702 565 194 SacII CC_GC'GG Cuts at: 0 37 59 1461 Size: 37 22 1402 Fragments arranged by size: 1402 37 22 SapI GCTCTTCn'nnn_ Cuts at: 0 211 1461 Size: 211 1250 SexAI A'CCwGG_T Cuts at: 0 1034 1461 Size: 1034 427 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1165 1461 Size: 1165 296 SmlI C'TyrA_G Cuts at: 0 793 1461 Size: 793 668 StuI AGG'CCT Cuts at: 0 1144 1461 Size: 1144 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1060 1406 1461 Size: 1060 346 55 Fragments arranged by size: 1060 346 55 TatI w'GTAC_w Cuts at: 0 683 1461 Size: 683 778 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1315 1347 1461 Size: 1315 32 114 Fragments arranged by size: 1315 114 32 XmnI GAAnn'nnTTC Cuts at: 0 991 1461 Size: 991 470 Enzymes that do cut: AccI Acc65I AcuI AflIII AhdI ApaI AvaI BanI BanII BbsI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV PfoI PspOMI PsrI PvuII SacII SapI SexAI SfiI SmlI StuI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BlpI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI