Restriction map for
pFN21AE1533
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MslI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcyI 1937 GACGCC G[GA]^CG[CT]C
AflII 889 CTTAAG C^TTAAG
AgeI 2216 ACCGGT A^CCGGT
Asp700I 1561 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 689 CTCGGG C^[CT]CG[GA]G
BfrI 889 CTTAAG C^TTAAG
BsaHI 1937 GACGCC G[GA]^CG[CT]C
BsaI 1509 GAGACC GGTCTC(1/5)
BsaWI 2216 ACCGGT [AT]^CCGG[AT]
BsmAI 1509 GAGAC GTCTC(1/5)
BsoBI 689 CTCGGG C^[CT]CG[GA]G
BspLU11I 516 ACATGT A^CATGT
BspMI 1473 ACCTGC ACCTGC(4/8)
BsrDI 501 CATTGC GCAATG(2/0)
CfoI 2016 GCGC GCG^C
DsaI 1203 CCATGG C^C[GA][CT]GG
HhaI 2016 GCGC GCG^C
Hin1I 1937 GACGCC G[GA]^CG[CT]C
HinP1I 2016 GCGC G^CGC
NcoI 1203 CCATGG C^CATGG
NspI 516 ACATGT [GA]CATG^[CT]
PflFI 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2216 ACCGGT A^CCGGT
PleI 326 GAGTC GAGTC(4/5)
SacI 1303 GAGCTC GAGCT^C
ScaI 1383 AGTACT AGT^ACT
StuI 626 AGGCCT AGG^CCT
Tth111I 433 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 938 CCAAATATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1561 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
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AflIII 516 ACATGT A^C[GA][CT]GT
2275 ACACGT
AocI 1645 CCTAAGG CC^T[ACGT]AGG
1960 CCTGAGG
AspHI 520 GTGCTC G[AT]GC[AT]^C
1303 GAGCTC
BalI 299 TGGCCA TGG^CCA
935 TGGCCA
BamHI 380 GGATCC G^GATCC
2110 GGATCC
BglII 264 AGATCT A^GATCT
1297 AGATCT
BlpI 1305 GCTCAGC GC^T[ACGT]AGC
1318 GCTGAGC
Bpu1102I 1305 GCTCAGC GC^T[ACGT]AGC
1318 GCTGAGC
BsgI 1137 CTGCAC GTGCAG(16/14)
1093 GTGCAG
BsiHKAI 520 GTGCTC G[AT]GC[AT]^C
1303 GAGCTC
BsmI 1101 GAATGC GAATGC(1/-1)
397 GCATTC
BstXI 866 CCAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
897 CCAAAACAGTGG
Bsu36I 1645 CCTAAGG CC^T[ACGT]AGG
1960 CCTGAGG
CelII 1305 GCTCAGC GC^T[ACGT]AGC
1318 GCTGAGC
DrdI 433 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2236 GACCTGTCTGTC
EaeI 299 TGGCCA [CT]^GGCC[GA]
935 TGGCCA
Eco81I 1645 CCTAAGG CC^T[ACGT]AGG
1960 CCTGAGG
EcoNI 549 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
648 CCTAGAAGAGG
HgaI 1527 GACGC GACGC(5/10)
1937 GACGC
HindIII 133 AAGCTT A^AGCTT
1790 AAGCTT
HphI 2165 TCACC GGTGA(8/7)
2270 TCACC
MluNI 299 TGGCCA TGG^CCA
935 TGGCCA
MscI 299 TGGCCA TGG^CCA
935 TGGCCA
PstI 697 CTGCAG CTGCA^G
830 CTGCAG
SapI 1534 GAAGAGC GCTCTTC(1/4)
1543 GAAGAGC
TaqI 723 TCGA T^CGA
1676 TCGA
TthHB8I 723 TCGA T^CGA
1676 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII