(Circular) (Six-base) MAPSORT of: pFN21AE1533.seq Check: 5847 from: 1 to: 7018 pFN21AE1533 7018 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4828 278 Size: 53 83 186 936 3292 2468 Fragments arranged by size: 3292 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4361 1937 Size: 2424 4594 Acc65I G'GTAC_C Cuts at: 4339 4339 Size: 7018 AclI AA'CG_TT Cuts at: 1563 5145 5518 1563 Size: 3582 373 3063 Fragments arranged by size: 3582 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 2259 2471 2669 4319 5081 6465 6733 Size: 599 28 212 198 1650 762 1384 268 Cuts at: 6733 1632 Size: 1917 Fragments arranged by size: 1917 1650 1384 762 599 268 212 198 28 AflII C'TTAA_G Cuts at: 820 1017 2894 820 Size: 197 1877 4944 Fragments arranged by size: 4944 1877 197 AflIII A'CryG_T Cuts at: 1203 2521 4280 5917 6894 1203 Size: 1318 1759 1637 977 1327 Fragments arranged by size: 1759 1637 1327 1318 977 AgeI A'CCGG_T Cuts at: 4221 5849 4221 Size: 1628 5390 AhdI GACnn_n'nnGTC Cuts at: 5747 5747 Size: 7018 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7018 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3123 1184 Size: 1939 5079 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3060 3092 3060 Size: 32 6986 AlwNI CAG_nnn'CTG Cuts at: 2535 3141 3535 4761 6333 2535 Size: 606 394 1226 1572 3220 Fragments arranged by size: 3220 1572 1226 606 394 ApaLI G'TGCA_C Cuts at: 5075 6231 5075 Size: 1156 5862 ApoI r'AATT_y Cuts at: 1493 2565 3258 3272 3991 4327 4553 4705 6814 Size: 1072 693 14 719 336 226 152 2109 Cuts at: 6814 1493 Size: 1697 Fragments arranged by size: 2109 1697 1072 719 693 336 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 5572 160 Size: 872 4540 1606 Fragments arranged by size: 4540 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7018 AvaI C'yCGr_G Cuts at: 1870 1943 2694 4343 1870 Size: 73 751 1649 4545 Fragments arranged by size: 4545 1649 751 73 BamHI G'GATC_C Cuts at: 2385 4115 4348 4715 2385 Size: 1730 233 367 4688 Fragments arranged by size: 4688 1730 367 233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4339 5794 618 Size: 325 217 627 2552 1455 1842 Fragments arranged by size: 2552 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2695 2701 3312 3501 4337 1391 Size: 1304 6 611 189 836 4072 Fragments arranged by size: 4072 1304 836 611 189 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2351 4215 4218 928 Size: 971 452 1864 3 3728 Fragments arranged by size: 3728 1864 971 452 3 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5209 5243 1903 Size: 34 3272 34 3678 Fragments arranged by size: 3678 3272 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4910 6120 4910 Size: 1210 5808 BclI T'GATC_A Cuts at: 1548 1548 Size: 7018 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5629 136 Size: 107 122 71 631 605 3957 1525 Fragments arranged by size: 3957 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2269 3302 7013 2269 Size: 1033 3711 2274 Fragments arranged by size: 3711 2274 1033 BlpI GC'TnA_GC Cuts at: 3311 3324 4435 3311 Size: 13 1111 5894 Fragments arranged by size: 5894 1111 13 Bme1580I G_kGCm'C Cuts at: 1790 5079 6235 1790 Size: 3289 1156 2573 Fragments arranged by size: 3289 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3874 4213 4283 1318 Size: 2556 339 70 4053 Fragments arranged by size: 4053 2556 339 70 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3921 4749 5707 Size: 416 53 83 399 24 2504 828 958 Cuts at: 5707 442 Size: 1753 Fragments arranged by size: 2504 1753 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7018 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2507 2576 2975 2999 5678 1239 Size: 1268 69 399 24 2679 2579 Fragments arranged by size: 2679 2579 1268 399 69 24 Bpu10I CC'TnA_GC Cuts at: 3134 3183 3134 Size: 49 6969 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2107 2297 3221 4216 5143 6008 6306 2107 Size: 190 924 995 927 865 298 2819 Fragments arranged by size: 2819 995 927 924 865 298 190 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3508 5681 882 Size: 617 2009 2173 2219 Fragments arranged by size: 2219 2173 2009 617 BsaAI yAC'GTr Cuts at: 493 1204 4809 493 Size: 711 3605 2702 Fragments arranged by size: 3605 2702 711 BsaBI GATnn'nnATC Cuts at: 7012 7012 Size: 7018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3943 4825 5207 5842 Size: 53 83 186 936 2410 882 382 635 Cuts at: 5842 275 Size: 1451 Fragments arranged by size: 2410 1451 936 882 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 5451 5849 6123 6270 6681 4221 Size: 1230 398 274 147 411 4558 Fragments arranged by size: 4558 1230 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2997 3027 3359 3389 3488 Size: 30 165 30 1594 30 332 30 99 Cuts at: 3488 3518 3678 3694 3708 3724 1178 Size: 30 160 16 14 16 4472 Fragments arranged by size: 4472 1594 332 165 160 99 30 30 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2510 2567 3056 3509 3761 3779 1186 Size: 1324 57 489 453 252 18 4425 Fragments arranged by size: 4425 1324 489 453 252 57 18 BseYI C'CCAG_C Cuts at: 3553 3803 3894 6221 6717 3553 Size: 250 91 2327 496 3854 Fragments arranged by size: 3854 2327 496 250 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3119 3127 1163 Size: 573 1383 8 5054 Fragments arranged by size: 5054 1383 573 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4468 5229 6257 1540 Size: 275 186 2467 761 1028 2301 Fragments arranged by size: 2467 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 3312 5079 5164 6235 2529 Size: 783 1767 85 1071 3312 Fragments arranged by size: 3312 1767 1071 783 85 BsmI GAATG_Cn' Cuts at: 2402 3112 4529 4622 2402 Size: 710 1417 93 4798 Fragments arranged by size: 4798 1417 710 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2529 2695 2701 3312 3501 4337 5079 Size: 399 739 166 6 611 189 836 742 Cuts at: 5079 5164 6235 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 836 742 739 611 399 189 166 85 6 BspEI T'CCGG_A Cuts at: 6681 6681 Size: 7018 BspHI T'CATG_A Cuts at: 4907 6637 4907 Size: 1730 5288 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3487 4373 844 Size: 2643 886 3489 Fragments arranged by size: 3489 2643 886 BsrBI CCG'CTC Cuts at: 1394 1823 4905 1394 Size: 429 3082 3507 Fragments arranged by size: 3507 3082 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 5513 5687 65 Size: 1183 1257 3008 174 1396 Fragments arranged by size: 3008 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2016 2457 3945 4221 5662 5849 1700 Size: 110 206 441 1488 276 1441 187 2869 Fragments arranged by size: 2869 1488 1441 441 276 206 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7018 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7018 BssSI C'ACGA_G Cuts at: 5078 6090 5078 Size: 1012 6006 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7018 BstBI TT'CG_AA Cuts at: 5822 5822 Size: 7018 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7018 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2909 2878 Size: 31 6987 BstYI r'GATC_y Cuts at: 1972 2269 2385 3302 4115 4348 4715 5100 5117 Size: 297 116 917 813 233 367 385 17 Cuts at: 5117 6569 6655 6667 7013 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 917 813 385 367 346 297 233 116 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3651 3966 3651 Size: 315 6703 BtgI C'CryG_G Cuts at: 513 1065 3208 513 Size: 552 2143 4323 Fragments arranged by size: 4323 2143 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6279 Fragments arranged by size: 6279 683 56 BtsI GCAGTG_nn' Cuts at: 4534 5328 5348 4534 Size: 794 20 6204 Fragments arranged by size: 6204 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7018 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3855 3890 529 Size: 35 3291 35 3657 Fragments arranged by size: 3657 3291 35 35 DraI TTT'AAA Cuts at: 4322 4322 Size: 7018 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2444 4247 6025 809 Size: 507 1128 1803 1778 1802 Fragments arranged by size: 1803 1802 1778 1128 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 2940 4465 Size: 54 1425 50 275 18 474 636 1525 Cuts at: 4465 5354 6983 8 Size: 889 1629 43 Fragments arranged by size: 1629 1525 1425 889 636 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4465 1537 Size: 275 2653 4090 Fragments arranged by size: 4090 2653 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2367 2652 2725 3033 3486 3534 3543 3756 Size: 1014 285 73 308 453 48 9 213 Cuts at: 3756 3833 4948 1353 Size: 77 1115 3423 Fragments arranged by size: 3423 1115 1014 453 308 285 213 77 73 48 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2194 5605 5979 6125 387 Size: 1064 138 605 3411 374 146 1280 Fragments arranged by size: 3411 1280 1064 605 374 146 138 EcoICRI GAG'CTC Cuts at: 3310 3310 Size: 7018 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2259 2471 2507 2576 2669 2975 Size: 393 599 28 212 36 69 93 306 Cuts at: 2975 2999 4319 5081 5678 6465 6733 1239 Size: 24 1320 762 597 787 268 1524 Fragments arranged by size: 1524 1320 787 762 599 597 393 306 268 212 93 69 36 28 24 EcoNI CCTnn'n_nnAGG Cuts at: 2558 2657 2558 Size: 99 6919 EcoO109I rG'GnC_Cy Cuts at: 2437 3178 3624 3971 2437 Size: 741 446 347 5484 Fragments arranged by size: 5484 741 446 347 EcoRI G'AATT_C Cuts at: 4327 4705 4327 Size: 378 6640 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3089 3089 Size: 7018 FspI TGC'GCA Cuts at: 5524 5524 Size: 7018 HaeII r_GCGC'y Cuts at: 1678 6165 1678 Size: 4487 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2116 2148 3294 3326 3359 Size: 32 135 32 544 32 1146 32 33 Cuts at: 3359 3391 5659 5691 5733 5765 1373 Size: 32 2268 32 42 32 2626 Fragments arranged by size: 2626 2268 1146 544 135 42 33 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4362 4608 669 Size: 988 281 2424 246 3079 Fragments arranged by size: 3079 2424 988 281 246 HindIII A'AGCT_T Cuts at: 748 2138 3795 748 Size: 1390 1657 3971 Fragments arranged by size: 3971 1657 1390 HpaI GTT'AAC Cuts at: 4608 4608 Size: 7018 KpnI G_GTAC'C Cuts at: 4343 4343 Size: 7018 MfeI C'AATT_G Cuts at: 4617 4617 Size: 7018 MluI A'CGCG_T Cuts at: 6894 6894 Size: 7018 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4218 6132 6316 1305 Size: 2913 1914 184 2007 Fragments arranged by size: 2913 2007 1914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2306 2942 10 Size: 54 1425 343 474 636 4086 Fragments arranged by size: 4086 1425 636 474 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4976 5335 5494 518 Size: 648 3810 359 159 2042 Fragments arranged by size: 3810 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2473 2815 2834 4037 4297 4434 5111 6259 Size: 867 342 19 1203 260 137 677 1148 Cuts at: 6259 6504 1606 Size: 245 2120 Fragments arranged by size: 2120 1203 1148 867 677 342 260 245 137 19 NcoI C'CATG_G Cuts at: 513 1065 3208 513 Size: 552 2143 4323 Fragments arranged by size: 4323 2143 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7018 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7018 NotI GC'GGCC_GC Cuts at: 4465 4465 Size: 7018 NspI r_CATG'y Cuts at: 1132 1229 2525 4376 5921 1132 Size: 97 1296 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 1296 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7018 PciI A'CATG_T Cuts at: 2521 5917 2521 Size: 3396 3622 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7018 PfoI T'CCnGG_A Cuts at: 3422 3422 Size: 7018 PmeI GTTT'AAAC Cuts at: 4322 4322 Size: 7018 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2511 2543 5087 5119 6600 6632 2511 Size: 32 2544 32 1481 32 2897 Fragments arranged by size: 2897 2544 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2437 3178 3624 3971 2437 Size: 741 446 347 5484 Fragments arranged by size: 5484 741 446 347 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7018 PsiI TTA'TAA Cuts at: 4588 4588 Size: 7018 PstI C_TGCA'G Cuts at: 830 2706 2839 4370 830 Size: 1876 133 1531 3478 Fragments arranged by size: 3478 1876 1531 133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7018 PvuII CAG'CTG Cuts at: 2473 2815 2834 4037 4297 2473 Size: 342 19 1203 260 5194 Fragments arranged by size: 5194 1203 342 260 19 SacI G_AGCT'C Cuts at: 3312 3312 Size: 7018 SalI G'TCGA_C Cuts at: 1936 4360 1936 Size: 2424 4594 SanDI GG'GwC_CC Cuts at: 2437 2437 Size: 7018 SapI GCTCTTCn'nnn_ Cuts at: 3534 3543 3534 Size: 9 7009 SbfI CC_TGCA'GG Cuts at: 4370 4370 Size: 7018 ScaI AGT'ACT Cuts at: 3390 5266 6978 3390 Size: 1876 1712 3430 Fragments arranged by size: 3430 1876 1712 SfcI C'TryA_G Cuts at: 826 1045 2702 2835 3509 3686 4311 4366 5501 Size: 219 1657 133 674 177 625 55 1135 Cuts at: 5501 6182 6373 826 Size: 681 191 1471 Fragments arranged by size: 1657 1471 1135 681 674 625 219 191 177 133 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7018 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7018 SmaI CCC'GGG Cuts at: 1872 4345 1872 Size: 2473 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2086 2276 2894 3200 4231 5122 Size: 197 926 143 190 618 306 1031 891 Cuts at: 5122 6023 6285 820 Size: 901 262 1553 Fragments arranged by size: 1553 1031 926 901 891 618 306 262 197 190 143 SnaBI TAC'GTA Cuts at: 493 4809 493 Size: 4316 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7018 SphI G_CATG'C Cuts at: 1132 4376 1132 Size: 3244 3774 SspI AAT'ATT Cuts at: 5 52 4942 5 Size: 47 4890 2081 Fragments arranged by size: 4890 2081 47 StuI AGG'CCT Cuts at: 1517 2633 1517 Size: 1116 5902 StyI C'CwwG_G Cuts at: 513 1065 2721 3208 4161 4457 513 Size: 552 1656 487 953 296 3074 Fragments arranged by size: 3074 1656 953 552 487 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4145 5045 5062 5215 5400 5845 325 Size: 1255 2565 900 17 153 185 445 1498 Fragments arranged by size: 2565 1498 1255 900 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3388 3526 5264 Size: 274 80 33 51 1443 1411 138 1738 Cuts at: 5264 6976 96 Size: 1712 138 Fragments arranged by size: 1738 1712 1443 1411 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2997 3029 3694 3726 6978 7010 2997 Size: 32 665 32 3252 32 3005 Fragments arranged by size: 3252 3005 665 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2441 1316 Size: 1125 5893 XbaI T'CTAG_A Cuts at: 4354 4354 Size: 7018 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2950 2950 Size: 7018 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7018 XmaI C'CCGG_G Cuts at: 1870 4343 1870 Size: 2473 4545 XmnI GAAnn'nnTTC Cuts at: 3570 5147 3570 Size: 1577 5441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4826 276 Size: 53 83 186 936 3292 2468 Fragments arranged by size: 3292 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI