################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AfaI 222 GTAC GT^AC AseI 460 ATTAAT AT^TAAT AsnI 460 ATTAAT AT^TAAT Asp700I 196 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 34 CTCGGG C^[CT]CG[GA]G BalI 341 TGGCCA TGG^CCA BanI 424 GGTGCC G^G[CT][GA]CC BlpI 233 GCTCAGC GC^T[ACGT]AGC Bpu1102I 233 GCTCAGC GC^T[ACGT]AGC BsaJI 506 CCTTGG C^C[ACGT][ACGT]GG BsmAI 439 GAGAC GTCTC(1/5) BsoBI 34 CTCGGG C^[CT]CG[GA]G BsrFI 575 GCCGGT [GA]^CCGG[CT] BsrI 43 ACTGG ACTGG(1/-1) BssKI 81 CCAGG ^CC[ACGT]GG BstAPI 448 GCAAAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 396 GGATG GGATG(2/0) BstNI 81 CCAGG CC^[AT]GG BstXI 516 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 497 AGATCC [GA]^GATC[CT] CelII 233 GCTCAGC GC^T[ACGT]AGC Cfr10I 575 GCCGGT [GA]^CCGG[CT] EaeI 341 TGGCCA [CT]^GGCC[GA] EarI 469 GAAGAG CTCTTC(1/4) EcoRII 81 CCAGG ^CC[AT]GG EcoT14I 506 CCTTGG C^C[AT][AT]GG FokI 396 GGATG GGATG(9/13) HaeIII 342 GGCC GG^CC HapII 576 CCGG C^CGG HpaII 576 CCGG C^CGG Ksp632I 469 GAAGAG CTCTTC(1/4) MflI 497 AGATCC [GA]^GATC[CT] MluNI 341 TGGCCA TGG^CCA MscI 341 TGGCCA TGG^CCA MseI 461 TTAA T^TAA MspI 576 CCGG C^CGG MvaI 81 CCAGG CC^[AT]GG PshBI 460 ATTAAT AT^TAAT PstI 239 CTGCAG CTGCA^G RsaI 222 GTAC GT^AC SapI 469 GAAGAGC GCTCTTC(1/4) ScrFI 81 CCAGG CC^[ACGT]GG SfcI 239 CTGCAG C^T[GA][CT]AG SgrAI 574 CGCCGGTG C[GA]^CCGG[CT]G StyI 506 CCTTGG C^C[AT][AT]GG Tru9I 461 TTAA T^TAA XhoII 497 AGATCC [GA]^GATC[CT] XmnI 196 GAACGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) 573 CCGC AlwI 498 GATCC GGATC(4/5) 84 GGATC AspHI 16 GAGCTC G[AT]GC[AT]^C 472 GAGCTC AvaII 322 GGTCC G^G[AT]CC 510 GGACC BanII 16 GAGCTC G[GA]GC[CT]^C 472 GAGCTC BbvI 241 GCAGC GCAGC(8/12) 238 GCTGC BmyI 16 GAGCTC G[AGT]GC[ACT]^C 472 GAGCTC BsiHKAI 16 GAGCTC G[AT]GC[AT]^C 472 GAGCTC BsmFI 38 GGGAC GGGAC(10/14) 179 GGGAC Bsp1286I 16 GAGCTC G[AGT]GC[ACT]^C 472 GAGCTC Cac8I 564 GCAAGC GC[ACGT]^[ACGT]GC 568 GCCAGC Cfr13I 322 GGTCC G^G[ACGT]CC 510 GGACC DpnII 85 GATC ^GATC 498 GATC MboI 85 GATC ^GATC 498 GATC MboII 291 GAAGA GAAGA(8/7) 469 GAAGA MslI 333 CATTGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 517 CATGAAAATG MspA1I 236 CAGCTG C[AC]G^C[GT]G 242 CAGCTG NdeII 85 GATC ^GATC 498 GATC PvuII 236 CAGCTG CAG^CTG 242 CAGCTG SacI 16 GAGCTC GAGCT^C 472 GAGCTC Sau3AI 85 GATC ^GATC 498 GATC Sau96I 322 GGTCC G^G[ACGT]CC 510 GGACC TseI 238 GCTGC G^C[AT]GC 241 GCAGC TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^ 183 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AluI BsiYI BslI DdeI Fnu4HI HinfI ItaI MnlI MwoI NlaIII NlaIV SfaNI TaqI TfiI TthHB8I