################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeIII MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AlwI 213 GGATC GGATC(4/5) AlwNI 285 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG BbvI 137 GCAGC GCAGC(8/12) BglII 156 AGATCT A^GATCT BsaAI 440 CACGTA [CT]AC^GT[GA] BsaJI 236 CCCTGG C^C[ACGT][ACGT]GG BsgI 135 GTGCAG GTGCAG(16/14) BsmFI 58 GTCCC GGGAC(10/14) BssKI 237 CCTGG ^CC[ACGT]GG BstNI 237 CCTGG CC^[AT]GG BstYI 156 AGATCT [GA]^GATC[CT] Cac8I 405 GCAGGC GC[ACGT]^[ACGT]GC DraIII 440 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRII 237 CCTGG ^CC[AT]GG EcoRV 301 GATATC GAT^ATC Fnu4HI 137 GCAGC GC^[ACGT]GC HindIII 34 AAGCTT A^AGCTT ItaI 137 GCAGC GC^[ACGT]GC MaeII 441 ACGT A^CGT MfeI 398 CAATTG C^AATTG MflI 156 AGATCT [GA]^GATC[CT] MslI 250 CATCGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 398 CAATTG C^AATTG MvaI 237 CCTGG CC^[AT]GG MwoI 36 GCTTCGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PstI 418 CTGCAG CTGCA^G ScrFI 237 CCTGG CC^[ACGT]GG SfcI 418 CTGCAG C^T[GA][CT]AG SmlI 145 CTTGAG C^T[CT][GA]AG TaqI 39 TCGA T^CGA TseI 137 GCAGC G^C[AT]GC TspRI 285 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 39 TCGA T^CGA XhoII 156 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### Asp700I 97 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 241 GAAATAATTC BfaI 185 CTAG C^TAG 298 CTAG BstF5I 374 CATCC GGATG(2/0) 339 GGATG FokI 374 CATCC GGATG(9/13) 339 GGATG MaeI 185 CTAG C^TAG 298 CTAG MseI 311 TTAA T^TAA 392 TTAA Tru9I 311 TTAA T^TAA 392 TTAA XmnI 97 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 241 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 8 enzymes) # ######################################### AluI DpnII MboI MboII MnlI NdeII Sau3AI Tsp509I