(Circular) (Six-base) MAPSORT of: pFN21AE6107.seq Check: 944 from: 1 to: 5176 pFN21AE6107 5176 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2986 278 Size: 53 83 186 936 1450 2468 Fragments arranged by size: 2468 1450 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2519 1937 Size: 582 4594 Acc65I G'GTAC_C Cuts at: 2497 2497 Size: 5176 AclI AA'CG_TT Cuts at: 1563 3303 3676 1563 Size: 1740 373 3063 Fragments arranged by size: 3063 1740 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 3239 4623 4891 1632 Size: 554 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1053 554 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4979 AflIII A'CryG_T Cuts at: 1203 4075 5052 1203 Size: 2872 977 1327 Fragments arranged by size: 2872 1327 977 AgeI A'CCGG_T Cuts at: 4007 4007 Size: 5176 AhdI GACnn_n'nnGTC Cuts at: 3905 3905 Size: 5176 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5176 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5176 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2352 2384 2352 Size: 32 5144 AlwNI CAG_nnn'CTG Cuts at: 2295 2919 4491 2295 Size: 624 1572 2980 Fragments arranged by size: 2980 1572 624 ApaLI G'TGCA_C Cuts at: 3233 4389 3233 Size: 1156 4020 ApoI r'AATT_y Cuts at: 1493 2485 2711 2863 4972 1493 Size: 992 226 152 2109 1697 Fragments arranged by size: 2109 1697 992 226 152 AseI AT'TA_AT Cuts at: 160 1032 3730 160 Size: 872 2698 1606 Fragments arranged by size: 2698 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5176 AvaI C'yCGr_G Cuts at: 1870 1943 2501 1870 Size: 73 558 4545 Fragments arranged by size: 4545 558 73 BamHI G'GATC_C Cuts at: 2506 2873 2506 Size: 367 4809 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 3952 618 Size: 325 217 627 710 1455 1842 Fragments arranged by size: 1842 1455 710 627 325 217 BanII G_rGCy'C Cuts at: 1391 2495 1391 Size: 1104 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4205 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3367 3401 1903 Size: 34 50 34 1346 34 3678 Fragments arranged by size: 3678 1346 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3068 4278 3068 Size: 1210 3966 BclI T'GATC_A Cuts at: 1548 1548 Size: 5176 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3787 136 Size: 107 122 71 631 605 2115 1525 Fragments arranged by size: 2115 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2161 5171 2161 Size: 3010 2166 BlpI GC'TnA_GC Cuts at: 2593 2593 Size: 5176 Bme1580I G_kGCm'C Cuts at: 1790 3237 4393 1790 Size: 1447 1156 2573 Fragments arranged by size: 2573 1447 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5176 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2907 3865 442 Size: 416 53 83 399 24 1490 958 1753 Fragments arranged by size: 1753 1490 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5176 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3836 1239 Size: 2597 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 3301 4166 4464 2171 Size: 1130 865 298 2883 Fragments arranged by size: 2883 1130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3839 882 Size: 617 2340 2219 Fragments arranged by size: 2340 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2447 2967 493 Size: 711 1243 520 2702 Fragments arranged by size: 2702 1243 711 520 BsaBI GATnn'nnATC Cuts at: 5170 5170 Size: 5176 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2983 3365 4000 275 Size: 53 83 186 936 1450 382 635 1451 Fragments arranged by size: 1451 1450 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3609 4007 4281 4428 4839 3609 Size: 398 274 147 411 3946 Fragments arranged by size: 3946 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2352 2382 1178 Size: 30 165 30 949 30 3972 Fragments arranged by size: 3972 949 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5176 BseYI C'CCAG_C Cuts at: 4379 4875 4379 Size: 496 4680 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2161 1163 Size: 573 425 4178 Fragments arranged by size: 4178 573 425 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2626 3387 4415 1540 Size: 275 186 625 761 1028 2301 Fragments arranged by size: 2301 1028 761 625 275 186 BsiHKAI G_wGCw'C Cuts at: 3237 3322 4393 3237 Size: 85 1071 4020 Fragments arranged by size: 4020 1071 85 BsmI GAATG_Cn' Cuts at: 2687 2780 2687 Size: 93 5083 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2495 3237 3322 4393 1391 Size: 399 705 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 705 399 85 BspEI T'CCGG_A Cuts at: 4839 4839 Size: 5176 BspHI T'CATG_A Cuts at: 3065 4795 3065 Size: 1730 3446 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2531 844 Size: 1687 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3063 1394 Size: 429 1240 3507 Fragments arranged by size: 3507 1240 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3671 3845 65 Size: 1183 2423 174 1396 Fragments arranged by size: 2423 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3820 4007 1700 Size: 110 2010 187 2869 Fragments arranged by size: 2869 2010 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5176 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5176 BssSI C'ACGA_G Cuts at: 3236 4248 3236 Size: 1012 4164 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5176 BstBI TT'CG_AA Cuts at: 3980 3980 Size: 5176 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5176 BstYI r'GATC_y Cuts at: 1972 2161 2506 2873 3258 3275 4727 4813 4825 Size: 189 345 367 385 17 1452 86 12 Cuts at: 4825 5171 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 345 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4624 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4437 Fragments arranged by size: 4437 683 56 BtsI GCAGTG_nn' Cuts at: 2296 2692 3486 3506 2296 Size: 396 794 20 3966 Fragments arranged by size: 3966 794 396 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5176 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5141 DraI TTT'AAA Cuts at: 2480 2480 Size: 5176 DraIII CAC_nnn'GTG Cuts at: 2450 2450 Size: 5176 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4183 809 Size: 507 2867 1802 Fragments arranged by size: 2867 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2623 3512 5141 Size: 54 1425 50 275 18 793 889 1629 Cuts at: 5141 8 Size: 43 Fragments arranged by size: 1629 1425 889 793 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2623 1537 Size: 275 811 4090 Fragments arranged by size: 4090 811 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3106 1353 Size: 1753 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3763 4137 4283 387 Size: 1064 138 2174 374 146 1280 Fragments arranged by size: 2174 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 3239 3836 4623 4891 1239 Size: 393 554 1053 597 787 268 1524 Fragments arranged by size: 1524 1053 787 597 554 393 268 EcoRI G'AATT_C Cuts at: 2485 2863 2485 Size: 378 4798 EcoRV GAT'ATC Cuts at: 2308 2308 Size: 5176 FspI TGC'GCA Cuts at: 3682 3682 Size: 5176 HaeII r_GCGC'y Cuts at: 1678 4323 1678 Size: 2645 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3817 3849 3891 3923 1373 Size: 32 135 32 2245 32 42 32 2626 Fragments arranged by size: 2626 2245 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2520 2766 669 Size: 988 281 582 246 3079 Fragments arranged by size: 3079 988 582 281 246 HindIII A'AGCT_T Cuts at: 748 2039 748 Size: 1291 3885 HpaI GTT'AAC Cuts at: 2766 2766 Size: 5176 KpnI G_GTAC'C Cuts at: 2501 2501 Size: 5176 MfeI C'AATT_G Cuts at: 2403 2775 2403 Size: 372 4804 MluI A'CGCG_T Cuts at: 5052 5052 Size: 5176 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4290 4474 1305 Size: 2985 184 2007 Fragments arranged by size: 2985 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3354 Fragments arranged by size: 3354 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 3134 3493 3652 518 Size: 648 1093 875 359 159 2042 Fragments arranged by size: 2042 1093 875 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2592 3269 4417 4662 1606 Size: 986 677 1148 245 2120 Fragments arranged by size: 2120 1148 986 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4624 NdeI CA'TA_TG Cuts at: 387 387 Size: 5176 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5176 NotI GC'GGCC_GC Cuts at: 2623 2623 Size: 5176 NspI r_CATG'y Cuts at: 1132 1229 2534 4079 1132 Size: 97 1305 1545 2229 Fragments arranged by size: 2229 1545 1305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5176 PciI A'CATG_T Cuts at: 4075 4075 Size: 5176 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5176 PmeI GTTT'AAAC Cuts at: 2480 2480 Size: 5176 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2091 2123 2352 2384 3245 3277 4758 4790 2091 Size: 32 229 32 861 32 1481 32 2477 Fragments arranged by size: 2477 1481 861 229 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5176 PsiI TTA'TAA Cuts at: 2746 2746 Size: 5176 PstI C_TGCA'G Cuts at: 830 2427 2528 830 Size: 1597 101 3478 Fragments arranged by size: 3478 1597 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5176 SalI G'TCGA_C Cuts at: 1936 2518 1936 Size: 582 4594 SbfI CC_TGCA'GG Cuts at: 2528 2528 Size: 5176 ScaI AGT'ACT Cuts at: 3424 5136 3424 Size: 1712 3464 SfcI C'TryA_G Cuts at: 826 1045 2423 2524 3659 4340 4531 826 Size: 219 1378 101 1135 681 191 1471 Fragments arranged by size: 1471 1378 1135 681 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5176 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5176 SmaI CCC'GGG Cuts at: 1872 2503 1872 Size: 631 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 3280 4181 4443 820 Size: 197 926 207 1130 901 262 1553 Fragments arranged by size: 1553 1130 926 901 262 207 197 SnaBI TAC'GTA Cuts at: 493 2967 493 Size: 2474 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5176 SphI G_CATG'C Cuts at: 1132 2534 1132 Size: 1402 3774 SspI AAT'ATT Cuts at: 5 52 3100 5 Size: 47 3048 2081 Fragments arranged by size: 3048 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5176 StyI C'CwwG_G Cuts at: 513 1065 2615 513 Size: 552 1550 3074 Fragments arranged by size: 3074 1550 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3203 3220 3373 3558 4003 325 Size: 1255 1623 17 153 185 445 1498 Fragments arranged by size: 1623 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3422 5134 96 Size: 274 80 33 51 1443 1445 1712 138 Fragments arranged by size: 1712 1445 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5136 5168 5136 Size: 32 5144 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5176 XbaI T'CTAG_A Cuts at: 2512 2512 Size: 5176 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5176 XmaI C'CCGG_G Cuts at: 1870 2501 1870 Size: 631 4545 XmnI GAAnn'nnTTC Cuts at: 2106 2250 3305 2106 Size: 144 1055 3977 Fragments arranged by size: 3977 1055 144 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2984 276 Size: 53 83 186 936 1450 2468 Fragments arranged by size: 2468 1450 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI