################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HindIII HinfI HpaI HphI KasI KpnI MaeI MaeIII MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 287 CGCG CG^CG AcyI 571 GACGCC G[GA]^CG[CT]C AlwNI 359 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 509 TGCGCA TGC^GCA BalI 242 TGGCCA TGG^CCA BanI 64 GGCACC G^G[CT][GA]CC BanII 528 GGGCTC G[GA]GC[CT]^C BbsI 544 GTCTTC GAAGAC(2/6) BbvCI 523 GCTGAGG CCTCAGC(-5/-2) BglI 235 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 544 GTCTTC GAAGAC(2/6) BsaHI 571 GACGCC G[GA]^CG[CT]C BseRI 427 GAGGAG GAGGAG(10/8) BsmFI 119 GGGAC GGGAC(10/14) BstF5I 231 GGATG GGATG(2/0) BstUI 287 CGCG CG^CG CfoI 510 GCGC GCG^C CpoI 130 CGGTCCG CG^G[AT]CCG DpnII 81 GATC ^GATC DraIII 66 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 49 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 236 CCATGG C^C[AT][AT]GG FokI 231 GGATG GGATG(9/13) FspI 509 TGCGCA TGC^GCA HhaI 510 GCGC GCG^C Hin1I 571 GACGCC G[GA]^CG[CT]C HinP1I 510 GCGC G^CGC HincII 517 GTCAAC GT[CT]^[GA]AC HindII 517 GTCAAC GT[CT]^[GA]AC KspI 286 CCGCGG CCGC^GG MboI 81 GATC ^GATC MluNI 242 TGGCCA TGG^CCA MscI 242 TGGCCA TGG^CCA MslI 30 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 287 CGCG CG^CG NcoI 236 CCATGG C^CATGG NdeII 81 GATC ^GATC NspI 221 ACATGC [GA]CATG^[CT] PflFI 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 119 GGGACCT [GA]G^G[AT]CC[CT] PvuII 193 CAGCTG CAG^CTG RsrII 130 CGGTCCG CG^G[AT]CCG SacII 286 CCGCGG CCGC^GG Sau3AI 81 GATC ^GATC SfaNI 232 GATGC GCATC(5/9) StyI 236 CCATGG C^C[AT][AT]GG Tth111I 538 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 404 CCAGCGGGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 120 GGACC G^G[AT]CC 131 GGTCC BcnI 116 CCGGG CC^[GC]GG 228 CCGGG BmyI 63 GGGCAC G[AGT]GC[ACT]^C 528 GGGCTC BpmI 205 CTGGAG CTGGAG(16/14) 448 CTGGAG BsiYI 49 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 285 CCCGCGGGAGG BslI 49 CCTGGCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 285 CCCGCGGGAGG Bsp1286I 63 GGGCAC G[AGT]GC[ACT]^C 528 GGGCTC BsrI 204 ACTGG ACTGG(1/-1) 69 CCAGT Cac8I 358 GCAGGC GC[ACGT]^[ACGT]GC 407 GCGGGC DraII 119 GGGACCT [GA]G^G[ACGT]CC[CT] 360 AGGCCCT EaeI 152 CGGCCA [CT]^GGCC[GA] 242 TGGCCA EarI 4 GAAGAG CTCTTC(1/4) 307 GAAGAG EcoO109I 119 GGGACCT [GA]G^G[ACGT]CC[CT] 360 AGGCCCT HapII 116 CCGG C^CGG 228 CCGG HgaI 355 GACGC GACGC(5/10) 571 GACGC HpaII 116 CCGG C^CGG 228 CCGG Ksp632I 4 GAAGAG CTCTTC(1/4) 307 GAAGAG MaeII 393 ACGT A^CGT 498 ACGT MspI 116 CCGG C^CGG 228 CCGG NciI 116 CCGGG CC^[GC]GG 228 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BbvI BsaI BsaJI BsgI BsmAI BssKI BstNI Cfr13I DdeI DsaI EcoRII FauI Fnu4HI HaeIII ItaI MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI TspRI