################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MseI MslI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 109 GACGTC GACGT^C AflIII 243 ACACGT A^C[GA][CT]GT AlwI 120 GATCC GGATC(4/5) AviII 425 TGCGCA TGC^GCA BanI 829 GGCGCC G^G[CT][GA]CC BanII 451 GAGCCC G[GA]GC[CT]^C BbeI 829 GGCGCC GGCGC^C BbsI 925 GTCTTC GAAGAC(2/6) BclI 95 TGATCA T^GATCA BfaI 197 CTAG C^TAG BglI 385 GCCTGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 187 CTCCAG CTGGAG(16/14) BpuAI 925 GTCTTC GAAGAC(2/6) BsaBI 546 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 214 GAGGAG GAGGAG(10/8) BsmAI 150 GTCTC GTCTC(1/5) Bsp1407I 605 TGTACA T^GTACA BsrGI 605 TGTACA T^GTACA BstAPI 201 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 119 AGATCC [GA]^GATC[CT] EarI 715 CTCTTC CTCTTC(1/4) FauI 431 CCCGC CCCGC(4/6) FbaI 95 TGATCA T^GATCA FspI 425 TGCGCA TGC^GCA HgaI 340 GACGC GACGC(5/10) HphI 448 GGTGA GGTGA(8/7) KasI 829 GGCGCC G^GCGCC Ksp632I 715 CTCTTC CTCTTC(1/4) MaeI 197 CTAG C^TAG MamI 546 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 119 AGATCC [GA]^GATC[CT] MspA1I 706 CCGCTG C[AC]G^C[GT]G NarI 829 GGCGCC GG^CGCC NcoI 177 CCATGG C^CATGG NspI 750 GCATGC [GA]CATG^[CT] PflMI 477 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SfaNI 182 GCATC GCATC(5/9) SfoI 829 GGCGCC GGC^GCC SmaI 344 CCCGGG CCC^GGG SphI 750 GCATGC GCATG^C SspBI 605 TGTACA T^GTACA StuI 191 AGGCCT AGG^CCT TaqI 107 TCGA T^CGA Tsp45I 158 GTGAC ^GT[GC]AC TthHB8I 107 TCGA T^CGA Van91I 477 CCACGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 41 CCAAGTTCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 119 AGATCC [GA]^GATC[CT] XmaCI 344 CCCGGG C^CCGGG XmaI 344 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 349 GAATTC [GA]^AATT[CT] 910 GAATTC Alw44I 358 GTGCAC G^TGCAC 406 GTGCAC AlwNI 56 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 202 CAGATTCTG ApaLI 358 GTGCAC G^TGCAC 406 GTGCAC ApoI 349 GAATTC [GA]^AATT[CT] 910 GAATTC AspHI 358 GTGCAC G[AT]GC[AT]^C 406 GTGCAC AvaI 86 CTCGGG C^[CT]CG[GA]G 344 CCCGGG BalI 392 TGGCCA TGG^CCA 768 TGGCCA BsiHKAI 358 GTGCAC G[AT]GC[AT]^C 406 GTGCAC BsoBI 86 CTCGGG C^[CT]CG[GA]G 344 CCCGGG BspMI 656 ACCTGC ACCTGC(4/8) 810 GCAGGT BstF5I 183 CATCC GGATG(2/0) 417 GGATG DraIII 409 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 478 CACGCCGTG EaeI 392 TGGCCA [CT]^GGCC[GA] 768 TGGCCA EcoRI 349 GAATTC G^AATTC 910 GAATTC EcoT14I 177 CCATGG C^C[AT][AT]GG 455 CCAAGG FokI 183 CATCC GGATG(9/13) 417 GGATG HapII 345 CCGG C^CGG 566 CCGG HinfI 204 GATTC G^A[ACGT]TC 258 GATTC HpaII 345 CCGG C^CGG 566 CCGG MaeII 110 ACGT A^CGT 245 ACGT MaeIII 82 GTTAC ^GT[ACGT]AC 158 GTGAC MluNI 392 TGGCCA TGG^CCA 768 TGGCCA MscI 392 TGGCCA TGG^CCA 768 TGGCCA MspI 345 CCGG C^CGG 566 CCGG PstI 208 CTGCAG CTGCA^G 571 CTGCAG StyI 177 CCATGG C^C[AT][AT]GG 455 CCAAGG TfiI 204 GATTC G^A[AT]TC 258 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcyI AfaI AluI AvaII BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI HaeII HaeIII HhaI Hin1I HinP1I ItaI MboI MboII MnlI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp509I TspRI