(Circular) (Six-base) MAPSORT of: pFN21AE6333.seq Check: 8805 from: 1 to: 5665 pFN21AE6333 5665 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2806 2806 Size: 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 3475 278 Size: 53 83 186 936 582 1357 2468 Fragments arranged by size: 2468 1357 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2894 3728 5112 5380 1632 Size: 491 771 834 1384 268 1917 Fragments arranged by size: 1917 1384 834 771 491 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 2248 4564 5541 1203 Size: 1045 2316 977 1327 Fragments arranged by size: 2316 1327 1045 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 4394 4394 Size: 5665 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 2066 2212 3408 4980 2066 Size: 146 1196 1572 2751 Fragments arranged by size: 2751 1572 1196 146 ApaLI G'TGCA_C Cuts at: 2363 2411 3722 4878 2363 Size: 48 1311 1156 3150 Fragments arranged by size: 3150 1311 1156 48 ApoI r'AATT_y Cuts at: 1493 2354 2915 2974 3200 3352 5461 1493 Size: 861 561 59 226 152 2109 1697 Fragments arranged by size: 2109 1697 861 561 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2091 2349 2990 1870 Size: 73 148 258 641 4545 Fragments arranged by size: 4545 641 258 148 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2834 2986 4441 618 Size: 325 217 627 1047 152 1455 1842 Fragments arranged by size: 1842 1455 1047 627 325 217 152 BanII G_rGCy'C Cuts at: 1391 2460 2984 1391 Size: 1069 524 4072 Fragments arranged by size: 4072 1069 524 BbeI G_GCGC'C Cuts at: 2838 2838 Size: 5665 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2923 928 Size: 971 1024 3670 Fragments arranged by size: 3670 1024 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 2100 1548 Size: 552 5113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2396 4276 136 Size: 107 122 71 631 605 724 1880 1525 Fragments arranged by size: 1880 1525 724 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2367 2415 3726 4882 1790 Size: 577 48 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 577 48 BmgBI CAC'GTC Cuts at: 1318 2251 1318 Size: 933 4732 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 4325 1239 Size: 938 2148 2579 Fragments arranged by size: 2579 2148 938 Bpu10I CC'TnA_GC Cuts at: 2448 2448 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3790 4655 4953 3790 Size: 865 298 4502 Fragments arranged by size: 4502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 2555 5659 2555 Size: 3104 2561 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2346 2835 3472 Size: 53 83 186 936 582 231 489 637 Cuts at: 3472 3854 4489 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 637 635 582 489 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2670 2700 1178 Size: 30 165 30 1267 30 4143 Fragments arranged by size: 4143 1267 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 1186 Size: 1048 4617 BseYI C'CCAG_C Cuts at: 2066 2168 2254 2864 4868 5364 2066 Size: 102 86 610 2004 496 2367 Fragments arranged by size: 2367 2004 610 496 102 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5092 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 2415 3726 3811 4882 2367 Size: 48 1311 85 1071 3150 Fragments arranged by size: 3150 1311 1071 85 48 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2415 2460 2984 3726 3811 4882 Size: 399 577 48 45 524 742 85 1071 Cuts at: 4882 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 577 524 399 85 48 45 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 2806 3020 844 Size: 1826 136 214 3489 Fragments arranged by size: 3489 1826 214 136 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4160 4334 65 Size: 1183 2912 174 1396 Fragments arranged by size: 2912 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 2610 96 Size: 2514 3151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 2212 794 Size: 1418 4247 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2124 2995 3362 3747 3764 5216 5302 5314 Size: 152 871 367 385 17 1452 86 12 Cuts at: 5314 5660 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 871 385 367 346 152 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2182 2418 2487 513 Size: 552 939 178 236 69 3691 Fragments arranged by size: 3691 939 552 236 178 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2167 2680 2935 3181 3975 3995 2067 Size: 100 513 255 246 794 20 3737 Fragments arranged by size: 3737 794 513 255 246 100 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DraIII CAC_nnn'GTG Cuts at: 2419 2488 2419 Size: 69 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2397 2773 3112 Size: 54 1425 50 275 18 567 376 339 Cuts at: 3112 4001 5630 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 567 376 339 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 3595 1353 Size: 1373 869 3423 Fragments arranged by size: 3423 1373 869 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2177 2894 3728 4325 5112 5380 Size: 393 491 54 717 834 597 787 268 Cuts at: 5380 1239 Size: 1524 Fragments arranged by size: 1524 834 787 717 597 491 393 268 54 EcoO109I rG'GnC_Cy Cuts at: 2513 2794 2854 2513 Size: 281 60 5324 Fragments arranged by size: 5324 281 60 EcoRI G'AATT_C Cuts at: 2354 2915 2974 3352 2354 Size: 561 59 378 4667 Fragments arranged by size: 4667 561 378 59 FspI TGC'GCA Cuts at: 2432 4171 2432 Size: 1739 3926 HaeII r_GCGC'y Cuts at: 1678 2099 2152 2838 4812 1678 Size: 421 53 686 1974 2531 Fragments arranged by size: 2531 1974 686 421 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2670 2702 4306 Size: 32 135 32 566 32 500 32 1604 Cuts at: 4306 4338 4380 4412 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1604 566 500 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KasI G'GCGC_C Cuts at: 2834 2834 Size: 5665 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2252 2722 4779 4963 1305 Size: 947 470 2057 184 2007 Fragments arranged by size: 2057 2007 947 470 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2399 2775 10 Size: 54 1425 343 567 376 2900 Fragments arranged by size: 2900 1425 567 376 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3623 3982 4141 518 Size: 648 2457 359 159 2042 Fragments arranged by size: 2457 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2713 3081 3758 4906 5151 1606 Size: 1107 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 1107 677 368 245 NarI GG'CG_CC Cuts at: 2835 2835 Size: 5665 NcoI C'CATG_G Cuts at: 513 1065 2004 2182 513 Size: 552 939 178 3996 Fragments arranged by size: 3996 939 552 178 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2759 3023 4568 1132 Size: 97 1530 264 1545 2229 Fragments arranged by size: 2229 1545 1530 264 97 PasI CC'CwG_GG Cuts at: 1348 2839 1348 Size: 1491 4174 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 4285 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 2217 2580 3017 830 Size: 1387 363 437 3478 Fragments arranged by size: 3478 1387 437 363 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 2213 2576 2590 2735 3013 4148 4829 Size: 219 1168 363 14 145 278 1135 681 Cuts at: 4829 5020 826 Size: 191 1471 Fragments arranged by size: 1471 1168 1135 681 363 278 219 191 145 14 SfoI GGC'GCC Cuts at: 2836 2836 Size: 5665 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2351 2992 1872 Size: 479 641 4545 Fragments arranged by size: 4545 641 479 SmlI C'TyrA_G Cuts at: 820 1017 1943 3769 4670 4932 820 Size: 197 926 1826 901 262 1553 Fragments arranged by size: 1826 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 2759 3023 1132 Size: 1627 264 3774 Fragments arranged by size: 3774 1627 264 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2198 1517 Size: 681 4984 StyI C'CwwG_G Cuts at: 513 1065 2004 2182 2460 3104 513 Size: 552 939 178 278 644 3074 Fragments arranged by size: 3074 939 644 552 278 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2610 3911 5623 Size: 274 80 33 51 1443 633 1301 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1712 1443 1301 633 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 5633 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2053 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2349 2990 1870 Size: 479 641 4545 Fragments arranged by size: 4545 641 479 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 3473 276 Size: 53 83 186 936 582 1357 2468 Fragments arranged by size: 2468 1357 936 582 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI