################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AclI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BbsI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MamI MfeI MluI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 794 TCCGGA T^CCGGA AcsI 219 GAATTT [GA]^AATT[CT] AcyI 256 GGCGCC G[GA]^CG[CT]C ApoI 219 GAATTT [GA]^AATT[CT] BalI 1043 TGGCCA TGG^CCA BamHI 194 GGATCC G^GATCC BanI 256 GGCGCC G^G[CT][GA]CC BbeI 256 GGCGCC GGCGC^C BciVI 84 GGATAC GTATCC(6/5) BsaHI 256 GGCGCC G[GA]^CG[CT]C BsaWI 794 TCCGGA [AT]^CCGG[AT] BseAI 794 TCCGGA T^CCGGA BsgI 653 GTGCAG GTGCAG(16/14) BsiEI 811 CGGTCG CG[GA][CT]^CG BspEI 794 TCCGGA T^CCGGA EcoNI 615 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 102 ATGCAT ATGCA^T HaeII 256 GGCGCC [GA]GCGC^[CT] Hin1I 256 GGCGCC G[GA]^CG[CT]C KasI 256 GGCGCC G^GCGCC MaeIII 46 GTCAC ^GT[ACGT]AC MluNI 1043 TGGCCA TGG^CCA MroI 794 TCCGGA T^CCGGA MscI 1043 TGGCCA TGG^CCA MslI 750 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 256 GGCGCC GG^CGCC NsiI 102 ATGCAT ATGCA^T PpuMI 866 AGGACCC [GA]G^G[AT]CC[CT] PstI 1351 CTGCAG CTGCA^G SacI 232 GAGCTC GAGCT^C SfaNI 104 GCATC GCATC(5/9) SfoI 256 GGCGCC GGC^GCC StuI 942 AGGCCT AGG^CCT TfiI 403 GATTC G^A[AT]TC Tsp45I 46 GTCAC ^GT[GC]AC Tsp509I 220 AATT ^AATT ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 622 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 1294 CAGGAGCTG ApaI 1130 GGGCCC GGGCC^C 1152 GGGCCC AspHI 232 GAGCTC G[AT]GC[AT]^C 892 GAGCAC BglI 660 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1315 GCCATTGAGGC BsiHKAI 232 GAGCTC G[AT]GC[AT]^C 892 GAGCAC BsmAI 172 GAGAC GTCTC(1/5) 210 GAGAC BsrBI 963 GAGCGG CCGCTC(-3/-3) 988 GAGCGG BstYI 194 GGATCC [GA]^GATC[CT] 791 AGATCC DpnII 195 GATC ^GATC 792 GATC EaeI 1043 TGGCCA [CT]^GGCC[GA] 1313 CGGCCA HphI 93 GGTGA GGTGA(8/7) 427 GGTGA MboI 195 GATC ^GATC 792 GATC MflI 194 GGATCC [GA]^GATC[CT] 791 AGATCC NdeII 195 GATC ^GATC 792 GATC Sau3AI 195 GATC ^GATC 792 GATC SfcI 752 CTGTAG C^T[GA][CT]AG 1351 CTGCAG SmaI 1133 CCCGGG CCC^GGG 1193 CCCGGG SmlI 322 CTTGAG C^T[CT][GA]AG 1207 CTTGAG XcmI 377 CCAAGGATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 768 CCACCCGAGCCTTGG XhoII 194 GGATCC [GA]^GATC[CT] 791 AGATCC XmaCI 1133 CCCGGG C^CCGGG 1193 CCCGGG XmaI 1133 CCCGGG C^CCGGG 1193 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflMI PleI PvuII SapI Sau96I ScrFI StyI TseI TspRI Van91I