################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MspA1I MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AluI 552 AGCT AG^CT AseI 475 ATTAAT AT^TAAT AsnI 475 ATTAAT AT^TAAT Asp700I 246 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 189 GGACC G^G[AT]CC BanI 283 GGTGCC G^G[CT][GA]CC BanII 747 GAGCCC G[GA]GC[CT]^C BfaI 421 CTAG C^TAG BpmI 43 CTGGAG CTGGAG(16/14) BseRI 19 GAGGAG GAGGAG(10/8) BsgI 819 CTGCAC GTGCAG(16/14) BsiYI 256 CCGTATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 256 CCGTATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 468 CATTGC GCAATG(2/0) BssHII 24 GCGCGC G^CGCGC BstXI 473 CCATTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 189 GGACC G^G[ACGT]CC DraII 188 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 803 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 457 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 188 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 511 GATATC GAT^ATC MaeI 421 CTAG C^TAG MaeII 836 ACGT A^CGT MfeI 356 CAATTG C^AATTG MslI 474 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 356 CAATTG C^AATTG PleI 53 GAGTC GAGTC(4/5) PpuMI 188 AGGACCC [GA]G^G[AT]CC[CT] PshBI 475 ATTAAT AT^TAAT Sau96I 189 GGACC G^G[ACGT]CC XcmI 776 CCAGAGCCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 246 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 25 CGCG CG^CG 30 CGCG AciI 29 CCGC CCGC(-3/-1) 5 GCGG AcsI 246 GAATTT [GA]^AATT[CT] 625 GAATTT AlwI 487 GATCC GGATC(4/5) 763 GATCC ApoI 246 GAATTT [GA]^AATT[CT] 625 GAATTT BbsI 304 GAAGAC GAAGAC(2/6) 55 GTCTTC BbvI 496 GCAGC GCAGC(8/12) 772 GCTGC BmyI 313 GGGCAC G[AGT]GC[ACT]^C 747 GAGCCC BpuAI 304 GAAGAC GAAGAC(2/6) 55 GTCTTC Bsp1286I 313 GGGCAC G[AGT]GC[ACT]^C 747 GAGCCC BspHI 96 TCATGA T^CATGA 669 TCATGA BstUI 25 CGCG CG^CG 30 CGCG Cac8I 24 GCGCGC GC[ACGT]^[ACGT]GC 817 GCCTGC DpnII 487 GATC ^GATC 763 GATC DraI 195 TTTAAA TTT^AAA 628 TTTAAA MaeIII 344 GTGAC ^GT[ACGT]AC 691 GTGAC MboI 487 GATC ^GATC 763 GATC MboII 304 GAAGA GAAGA(8/7) 56 TCTTC MvnI 25 CGCG CG^CG 30 CGCG NdeII 487 GATC ^GATC 763 GATC RcaI 96 TCATGA T^CATGA 669 TCATGA Sau3AI 487 GATC ^GATC 763 GATC TfiI 716 GAATC G^A[AT]TC 811 GAATC TseI 496 GCAGC G^C[AT]GC 772 GCTGC Tsp45I 344 GTGAC ^GT[GC]AC 691 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI BsaJI BsrI BssKI BstF5I BstNI CfoI DdeI EcoRII Fnu4HI FokI HhaI HinP1I HinfI HphI ItaI MnlI MseI MvaI MwoI NlaIII NlaIV RsaI ScrFI SfaNI Tru9I Tsp509I