(Circular) (Six-base) MAPSORT of: pFN21AE1506.seq Check: 5990 from: 1 to: 5788 pFN21AE1506 5788 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2272 2272 Size: 5788 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 3131 1937 Size: 1078 116 4594 Fragments arranged by size: 4594 1078 116 Acc65I G'GTAC_C Cuts at: 2969 3109 2969 Size: 140 5648 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3004 3083 3851 5235 5503 1632 Size: 1372 79 768 1384 268 1917 Fragments arranged by size: 1917 1384 1372 768 268 79 AflII C'TTAA_G Cuts at: 820 1017 2221 820 Size: 197 1204 4387 Fragments arranged by size: 4387 1204 197 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2246 2986 1166 Size: 1080 740 3968 Fragments arranged by size: 3968 1080 740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaI G_GGCC'C Cuts at: 2112 2112 Size: 5788 ApaLI G'TGCA_C Cuts at: 2069 3845 5001 2069 Size: 1776 1156 2856 Fragments arranged by size: 2856 1776 1156 ApoI r'AATT_y Cuts at: 1493 2174 2519 2568 3097 3323 3475 5584 1493 Size: 681 345 49 529 226 152 2109 1697 Fragments arranged by size: 2109 1697 681 529 345 226 152 49 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2081 3044 3113 1870 Size: 73 138 963 69 4545 Fragments arranged by size: 4545 963 138 73 69 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2969 3109 4564 618 Size: 325 217 627 1182 140 1455 1842 Fragments arranged by size: 1842 1455 1182 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2112 2374 3107 1391 Size: 721 262 733 4072 Fragments arranged by size: 4072 733 721 262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2073 2112 3849 5005 1790 Size: 283 39 1737 1156 2573 Fragments arranged by size: 2573 1737 1156 283 39 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2841 4448 1239 Size: 1602 1607 2579 Fragments arranged by size: 2579 1607 1602 Bpu10I CC'TnA_GC Cuts at: 2559 2754 2559 Size: 195 5593 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2894 3913 4778 5076 2894 Size: 1019 865 298 3606 Fragments arranged by size: 3606 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 4451 882 Size: 617 585 2367 2219 Fragments arranged by size: 2367 2219 617 585 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2655 3027 1186 Size: 1291 178 372 3947 Fragments arranged by size: 3947 1291 372 178 BseYI C'CCAG_C Cuts at: 2577 4991 5487 2577 Size: 2414 496 2878 Fragments arranged by size: 2878 2414 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2964 1163 Size: 573 1228 3987 Fragments arranged by size: 3987 1228 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 3849 3934 5005 2073 Size: 1776 85 1071 2856 Fragments arranged by size: 2856 1776 1071 85 BsmI GAATG_Cn' Cuts at: 2645 3299 3392 2645 Size: 654 93 5041 Fragments arranged by size: 5041 654 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2112 2374 3107 3849 3934 5005 Size: 399 283 39 262 733 742 85 1071 Cuts at: 5005 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 733 399 283 262 85 39 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 2961 3677 5407 2961 Size: 716 1730 3342 Fragments arranged by size: 3342 1730 716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2272 2798 3143 844 Size: 1428 526 345 3489 Fragments arranged by size: 3489 1428 526 345 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2532 4283 4457 65 Size: 1183 1284 1751 174 1396 Fragments arranged by size: 1751 1396 1284 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 3148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 2068 794 Size: 1274 4514 BstBI TT'CG_AA Cuts at: 2566 4592 2566 Size: 2026 3762 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstYI r'GATC_y Cuts at: 1972 3118 3485 3870 3887 5339 5425 5437 5783 Size: 1146 367 385 17 1452 86 12 346 Cuts at: 5783 1972 Size: 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2112 2154 513 Size: 552 951 96 42 4147 Fragments arranged by size: 4147 951 552 96 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2241 2770 2899 3304 4098 4118 2241 Size: 529 129 405 794 20 3911 Fragments arranged by size: 3911 794 529 405 129 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 2434 2926 3092 2434 Size: 492 166 5130 Fragments arranged by size: 5130 492 166 DraIII CAC_nnn'GTG Cuts at: 2204 2204 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753 Size: 54 1425 50 275 18 1405 889 1629 Cuts at: 5753 8 Size: 43 Fragments arranged by size: 1629 1425 1405 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3718 1353 Size: 801 1564 3423 Fragments arranged by size: 3423 1564 801 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2841 3004 3083 3851 4448 5235 5503 Size: 393 1209 163 79 768 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1209 787 768 597 393 268 163 79 EcoO109I rG'GnC_Cy Cuts at: 2086 2108 2086 Size: 22 5766 EcoRI G'AATT_C Cuts at: 2519 2568 3097 3475 2519 Size: 49 529 378 4832 Fragments arranged by size: 4832 529 378 49 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2434 3034 2434 Size: 600 5188 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373 Size: 32 135 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3081 3132 3378 669 Size: 988 281 1143 51 246 3079 Fragments arranged by size: 3079 1143 988 281 246 51 HindIII A'AGCT_T Cuts at: 748 3071 748 Size: 2323 3465 HpaI GTT'AAC Cuts at: 3081 3378 3081 Size: 297 5491 KpnI G_GTAC'C Cuts at: 2973 3113 2973 Size: 140 5648 MfeI C'AATT_G Cuts at: 2835 3387 2835 Size: 552 5236 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2399 4902 5086 1305 Size: 1094 2503 184 2007 Fragments arranged by size: 2503 2007 1094 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2696 2986 3746 4105 4264 518 Size: 648 1080 450 290 760 359 159 2042 Fragments arranged by size: 2042 1080 760 648 450 359 290 159 MspA1I CmG'CkG Cuts at: 1606 2065 2788 3204 3881 5029 5274 1606 Size: 459 723 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 723 677 459 416 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5236 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 2105 1348 Size: 757 5031 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2086 2086 Size: 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2108 2108 Size: 5788 PstI C_TGCA'G Cuts at: 830 2064 3140 830 Size: 1234 1076 3478 Fragments arranged by size: 3478 1234 1076 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 2065 2788 2065 Size: 723 5065 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2060 2984 3136 4271 4952 5143 826 Size: 219 1015 924 152 1135 681 191 1471 Fragments arranged by size: 1471 1135 1015 924 681 219 191 152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2221 2909 3892 4793 5055 820 Size: 197 926 278 688 983 901 262 1553 Fragments arranged by size: 1553 983 926 901 688 278 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2637 1517 Size: 1120 4668 StyI C'CwwG_G Cuts at: 513 1065 2383 3227 513 Size: 552 1318 844 3074 Fragments arranged by size: 3074 1318 844 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2298 2736 Size: 274 80 33 51 1443 186 135 438 Cuts at: 2736 4034 5746 96 Size: 1298 1712 138 Fragments arranged by size: 1712 1443 1298 438 274 186 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 2042 3917 2042 Size: 1875 3913 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI