Restriction map for
pFN21AE2545
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# NO CUT ENZYMES ( 151 enzymes) #
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Acc65I AccI AccII AccIII AclI
AfaI AgeI AhdI Alw44I AlwNI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CelII ClaI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 295 GACGTC GACGT^C
AcsI 256 GAATTC [GA]^AATT[CT]
AflII 307 CTTAAG C^TTAAG
AflIII 119 ACACGT A^C[GA][CT]GT
AluI 305 AGCT AG^CT
AlwI 426 GATCC GGATC(4/5)
AocI 126 CCTGAGG CC^T[ACGT]AGG
Aor51HI 557 AGCGCT AGC^GCT
ApoI 256 GAATTC [GA]^AATT[CT]
AspI 591 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 200 CCCGAG C^[CT]CG[GA]G
BanI 541 GGTGCC G^G[CT][GA]CC
BbsI 532 GAAGAC GAAGAC(2/6)
BbvCI 230 CCTCAGC CCTCAGC(-5/-2)
BfaI 505 CTAG C^TAG
BfrI 307 CTTAAG C^TTAAG
BpmI 430 CTGGAG CTGGAG(16/14)
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaI 25 GAGACC GGTCTC(1/5)
BsiEI 10 CGGTCG CG[GA][CT]^CG
BsoBI 200 CCCGAG C^[CT]CG[GA]G
BsrFI 8 GCCGGT [GA]^CCGG[CT]
BsrI 496 CCAGT ACTGG(1/-1)
BstF5I 393 GGATG GGATG(2/0)
BstYI 425 AGATCC [GA]^GATC[CT]
Bsu36I 126 CCTGAGG CC^T[ACGT]AGG
CfoI 558 GCGC GCG^C
Cfr10I 8 GCCGGT [GA]^CCGG[CT]
CpoI 14 CGGTCCG CG^G[AT]CCG
DpnII 426 GATC ^GATC
EaeI 68 CGGCCA [CT]^GGCC[GA]
EarI 526 GAAGAG CTCTTC(1/4)
Eco47III 557 AGCGCT AGC^GCT
Eco81I 126 CCTGAGG CC^T[ACGT]AGG
EcoNI 480 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 256 GAATTC G^AATTC
FokI 393 GGATG GGATG(9/13)
HaeII 557 AGCGCT [GA]GCGC^[CT]
HgaI 595 GCGTC GACGC(5/10)
HhaI 558 GCGC GCG^C
HinP1I 558 GCGC G^CGC
Ksp632I 526 GAAGAG CTCTTC(1/4)
MaeI 505 CTAG C^TAG
MboI 426 GATC ^GATC
MflI 425 AGATCC [GA]^GATC[CT]
NdeII 426 GATC ^GATC
PflFI 591 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 510 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 14 CGGTCCG CG^G[AT]CCG
Sau3AI 426 GATC ^GATC
SfaNI 226 GATGC GCATC(5/9)
TspRI 581 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 591 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 425 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 295 GACGTC G[GA]^CG[CT]C
594 GGCGTC
Asp700I 493 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
555 GAAGCGCTTC
AvaII 15 GGTCC G^G[AT]CC
570 GGACC
BanII 363 GAGCCC G[GA]GC[CT]^C
436 GAGCCC
BcnI 18 CCGGG CC^[GC]GG
36 CCGGG
BsaHI 295 GACGTC G[GA]^CG[CT]C
594 GGCGTC
BseRI 450 CTCCTC GAGGAG(10/8)
433 GAGGAG
BsmAI 25 GAGAC GTCTC(1/5)
508 GAGAC
BsmFI 123 GTCCC GGGAC(10/14)
357 GTCCC
EcoT14I 41 CCAAGG C^C[AT][AT]GG
485 CCAAGG
FauI 29 CCCGC CCCGC(4/6)
546 CCCGC
Hin1I 295 GACGTC G[GA]^CG[CT]C
594 GGCGTC
MaeII 121 ACGT A^CGT
296 ACGT
MseI 137 TTAA T^TAA
308 TTAA
NciI 18 CCGGG CC^[GC]GG
36 CCGGG
SmlI 307 CTTAAG C^T[CT][GA]AG
330 CTCAAG
StyI 41 CCAAGG C^C[AT][AT]GG
485 CCAAGG
TaqI 74 TCGA T^CGA
184 TCGA
Tru9I 137 TTAA T^TAA
308 TTAA
Tsp509I 238 AATT ^AATT
257 AATT
TthHB8I 74 TCGA T^CGA
184 TCGA
XmnI 493 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
555 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MspI MvaI MwoI
NlaIV PleI Sau96I ScrFI TseI
Tsp45I