(Circular) (Six-base) MAPSORT of: pFN21AE2545.seq Check: 1096 from: 1 to: 5311 pFN21AE2545 5311 bp With 182 enzymes: SgfI * September 30, 2014 11:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2304 3121 278 Size: 53 83 186 936 768 817 2468 Fragments arranged by size: 2468 936 817 768 186 83 53 AccI GT'mk_AC Cuts at: 1937 2654 1937 Size: 717 4594 Acc65I G'GTAC_C Cuts at: 2632 2632 Size: 5311 AclI AA'CG_TT Cuts at: 1563 3438 3811 1563 Size: 1875 373 3063 Fragments arranged by size: 3063 1875 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 3374 4758 5026 1632 Size: 947 795 1384 268 1917 Fragments arranged by size: 1917 1384 947 795 268 AfeI AGC'GCT Cuts at: 2564 2564 Size: 5311 AflII C'TTAA_G Cuts at: 820 1017 2312 820 Size: 197 1295 3819 Fragments arranged by size: 3819 1295 197 AflIII A'CryG_T Cuts at: 1203 2124 4210 5187 1203 Size: 921 2086 977 1327 Fragments arranged by size: 2086 1327 977 921 AgeI A'CCGG_T Cuts at: 4142 4142 Size: 5311 AhdI GACnn_n'nnGTC Cuts at: 4040 4040 Size: 5311 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5311 AlwNI CAG_nnn'CTG Cuts at: 3054 4626 3054 Size: 1572 3739 ApaLI G'TGCA_C Cuts at: 3368 4524 3368 Size: 1156 4155 ApoI r'AATT_y Cuts at: 1493 2261 2620 2846 2998 5107 1493 Size: 768 359 226 152 2109 1697 Fragments arranged by size: 2109 1697 768 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 3865 160 Size: 872 2833 1606 Fragments arranged by size: 2833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 AvaI C'yCGr_G Cuts at: 1870 1943 2205 2636 1870 Size: 73 262 431 4545 Fragments arranged by size: 4545 431 262 73 BamHI G'GATC_C Cuts at: 2641 3008 2641 Size: 367 4944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2546 2632 4087 618 Size: 325 217 627 759 86 1455 1842 Fragments arranged by size: 1842 1455 759 627 325 217 86 BanII G_rGCy'C Cuts at: 1391 2372 2445 2630 1391 Size: 981 73 185 4072 Fragments arranged by size: 4072 981 185 73 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 928 Size: 971 645 3695 Fragments arranged by size: 3695 971 645 BbvCI CC'TCA_GC Cuts at: 2236 2236 Size: 5311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3502 3536 1903 Size: 34 1565 34 3678 Fragments arranged by size: 3678 1565 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3203 4413 3203 Size: 1210 4101 BclI T'GATC_A Cuts at: 1548 1548 Size: 5311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3922 136 Size: 107 122 71 631 605 2250 1525 Fragments arranged by size: 2250 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5306 5306 Size: 5311 BlpI GC'TnA_GC Cuts at: 2728 2728 Size: 5311 Bme1580I G_kGCm'C Cuts at: 1790 2551 3372 4528 1790 Size: 761 821 1156 2573 Fragments arranged by size: 2573 1156 821 761 BmgBI CAC'GTC Cuts at: 1318 2127 1318 Size: 809 4502 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3042 4000 442 Size: 416 53 83 399 24 1625 958 1753 Fragments arranged by size: 1753 1625 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2461 2557 2461 Size: 96 5215 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 3971 1239 Size: 1217 1515 2579 Fragments arranged by size: 2579 1515 1217 Bpu10I CC'TnA_GC Cuts at: 2236 2366 2236 Size: 130 5181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2320 3436 4301 4599 2320 Size: 1116 865 298 3032 Fragments arranged by size: 3032 1116 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 3974 882 Size: 617 525 1950 2219 Fragments arranged by size: 2219 1950 617 525 BsaAI yAC'GTr Cuts at: 493 1204 3102 493 Size: 711 1898 2702 Fragments arranged by size: 2702 1898 711 BsaBI GATnn'nnATC Cuts at: 5305 5305 Size: 5311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2301 2600 3118 3500 Size: 53 83 186 936 768 299 518 382 Cuts at: 3500 4135 275 Size: 635 1451 Fragments arranged by size: 1451 936 768 635 518 382 299 186 83 53 BsaWI w'CCGG_w Cuts at: 3744 4142 4416 4563 4974 3744 Size: 398 274 147 411 4081 Fragments arranged by size: 4081 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 1178 Size: 30 165 30 687 30 4369 Fragments arranged by size: 4369 687 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2446 2453 1186 Size: 1260 7 4044 Fragments arranged by size: 4044 1260 7 BseYI C'CCAG_C Cuts at: 2342 2349 2371 2406 2419 4514 5010 2342 Size: 7 22 35 13 2095 496 2643 Fragments arranged by size: 2643 2095 496 35 22 13 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4738 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2761 3522 4550 1540 Size: 275 186 17 743 761 1028 2301 Fragments arranged by size: 2301 1028 761 743 275 186 17 BsiHKAI G_wGCw'C Cuts at: 3372 3457 4528 3372 Size: 85 1071 4155 Fragments arranged by size: 4155 1071 85 BsmI GAATG_Cn' Cuts at: 2822 2915 2822 Size: 93 5218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2372 2445 2551 2630 3372 3457 4528 Size: 399 582 73 106 79 742 85 1071 Cuts at: 4528 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 582 399 106 85 79 73 BspEI T'CCGG_A Cuts at: 4974 4974 Size: 5311 BspHI T'CATG_A Cuts at: 3200 4930 3200 Size: 1730 3581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2666 844 Size: 1822 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3198 1394 Size: 429 1375 3507 Fragments arranged by size: 3507 1375 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3806 3980 65 Size: 1183 2558 174 1396 Fragments arranged by size: 2558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 3955 4142 1700 Size: 110 203 1942 187 2869 Fragments arranged by size: 2869 1942 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5311 BssSI C'ACGA_G Cuts at: 3371 4383 3371 Size: 1012 4299 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5311 BstBI TT'CG_AA Cuts at: 4115 4115 Size: 5311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5311 BstYI r'GATC_y Cuts at: 1972 2430 2641 3008 3393 3410 4862 4948 4960 Size: 458 211 367 385 17 1452 86 12 Cuts at: 4960 5306 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 211 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2132 2132 Size: 5311 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4759 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4572 Fragments arranged by size: 4572 683 56 BtsI GCAGTG_nn' Cuts at: 2827 3621 3641 2827 Size: 794 20 4497 Fragments arranged by size: 4497 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5276 DraI TTT'AAA Cuts at: 2615 2615 Size: 5311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4318 809 Size: 507 3002 1802 Fragments arranged by size: 3002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2758 3647 Size: 54 1425 50 275 18 243 685 889 Cuts at: 3647 5276 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 685 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2758 1537 Size: 275 946 4090 Fragments arranged by size: 4090 946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2526 3241 1353 Size: 1173 715 3423 Fragments arranged by size: 3423 1173 715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 3898 4272 4418 387 Size: 1064 138 453 1856 374 146 1280 Fragments arranged by size: 1856 1280 1064 453 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2456 2579 3374 3971 4758 5026 1239 Size: 393 824 123 795 597 787 268 1524 Fragments arranged by size: 1524 824 795 787 597 393 268 123 EcoNI CCTnn'n_nnAGG Cuts at: 2489 2489 Size: 5311 EcoRI G'AATT_C Cuts at: 2261 2620 2998 2261 Size: 359 378 4574 Fragments arranged by size: 4574 378 359 FspI TGC'GCA Cuts at: 3817 3817 Size: 5311 HaeII r_GCGC'y Cuts at: 1678 2566 4458 1678 Size: 888 1892 2531 Fragments arranged by size: 2531 1892 888 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3952 3984 4026 4058 1373 Size: 32 135 32 2380 32 42 32 2626 Fragments arranged by size: 2626 2380 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2655 2901 669 Size: 988 281 717 246 3079 Fragments arranged by size: 3079 988 717 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5311 HpaI GTT'AAC Cuts at: 2901 2901 Size: 5311 KpnI G_GTAC'C Cuts at: 2636 2636 Size: 5311 MfeI C'AATT_G Cuts at: 2910 2910 Size: 5311 MluI A'CGCG_T Cuts at: 5187 5187 Size: 5311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 4425 4609 1305 Size: 1104 2016 184 2007 Fragments arranged by size: 2016 2007 1104 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3489 Fragments arranged by size: 3489 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3269 3628 3787 518 Size: 648 2103 359 159 2042 Fragments arranged by size: 2103 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2727 3404 4552 4797 1606 Size: 1121 677 1148 245 2120 Fragments arranged by size: 2120 1148 1121 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4759 NdeI CA'TA_TG Cuts at: 387 387 Size: 5311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5311 NotI GC'GGCC_GC Cuts at: 2758 2758 Size: 5311 NspI r_CATG'y Cuts at: 1132 1229 2669 4214 1132 Size: 97 1440 1545 2229 Fragments arranged by size: 2229 1545 1440 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5311 PciI A'CATG_T Cuts at: 4210 4210 Size: 5311 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5311 PfoI T'CCnGG_A Cuts at: 2265 2433 2265 Size: 168 5143 PmeI GTTT'AAAC Cuts at: 2615 2615 Size: 5311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 4893 4925 3380 Size: 32 1481 32 3766 Fragments arranged by size: 3766 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2519 1613 Size: 906 4405 PsiI TTA'TAA Cuts at: 2881 2881 Size: 5311 PstI C_TGCA'G Cuts at: 830 2663 830 Size: 1833 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5311 RsrII CG'GwC_CG Cuts at: 2020 2020 Size: 5311 SalI G'TCGA_C Cuts at: 1936 2653 1936 Size: 717 4594 SbfI CC_TGCA'GG Cuts at: 2663 2663 Size: 5311 ScaI AGT'ACT Cuts at: 3559 5271 3559 Size: 1712 3599 SfcI C'TryA_G Cuts at: 826 1045 2659 3794 4475 4666 826 Size: 219 1614 1135 681 191 1471 Fragments arranged by size: 1614 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5311 SmaI CCC'GGG Cuts at: 1872 2638 1872 Size: 766 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 2335 3415 4316 4578 820 Size: 197 926 369 23 1080 901 262 1553 Fragments arranged by size: 1553 1080 926 901 369 262 197 23 SnaBI TAC'GTA Cuts at: 493 3102 493 Size: 2609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5311 SphI G_CATG'C Cuts at: 1132 2669 1132 Size: 1537 3774 SspI AAT'ATT Cuts at: 5 52 3235 5 Size: 47 3183 2081 Fragments arranged by size: 3183 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5311 StyI C'CwwG_G Cuts at: 513 1065 2046 2490 2750 513 Size: 552 981 444 260 3074 Fragments arranged by size: 3074 981 552 444 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 2098 2108 3338 3355 3508 3693 Size: 1255 428 90 10 1230 17 153 185 Cuts at: 3693 4138 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1230 445 428 185 153 90 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3557 5269 96 Size: 274 80 33 51 1443 1580 1712 138 Fragments arranged by size: 1712 1580 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 5271 5303 2090 Size: 32 3149 32 2098 Fragments arranged by size: 3149 2098 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2599 1316 Size: 1283 4028 XbaI T'CTAG_A Cuts at: 2647 2647 Size: 5311 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5311 XmaI C'CCGG_G Cuts at: 1870 2636 1870 Size: 766 4545 XmnI GAAnn'nnTTC Cuts at: 2502 2564 3440 2502 Size: 62 876 4373 Fragments arranged by size: 4373 876 62 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2302 3119 276 Size: 53 83 186 936 768 817 2468 Fragments arranged by size: 2468 936 817 768 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI